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Novel Protocol for Acute In Situ Ecotoxicity Test Using Native Crustaceans Applied to Groundwater Ecosystems

1
Natural History Museum of Denmark, University of Copenhagen, 2100 Copenhagen, Denmark
2
Univ Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR 5023 LEHNA, F-69622 Villeurbanne, France
3
Faculty of Chemistry and Chemical Technology, University of Ljubljana, SI-1000 Ljubljana, Slovenia
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Centre for Ecology, Evolution and Environmental Changes (cE3c) and Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisbon, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Lorenzo Proia
Water 2021, 13(8), 1132; https://doi.org/10.3390/w13081132
Received: 22 March 2021 / Revised: 12 April 2021 / Accepted: 16 April 2021 / Published: 20 April 2021
(This article belongs to the Section Aquatic Systems—Quality and Contamination)
Current standardized laboratory test protocols use model species that have limitations to accurately assess native species responses to stressors. We developed and tested a novel acute in situ protocol for testing field-collected organisms. We used Asellus aquaticus and NaCl as a reference toxicant to test for the effects of location (laboratory vs. in situ), medium (synthetic vs. field water), substrate (presence vs. absence), and protocol replicability. We further tested the protocol using groundwater-adapted isopods: Proasellus assaforensis for the effect of location, P. cavaticus of medium and P.lusitanicus of substrate. Our results showed that A.aquaticus’ lethality obtained with the novel acute in situ protocol did not significantly differ from those from laboratory testing. However, laboratory tested P.assaforensis showed a higher sensitivity, suggesting that its acclimation to laboratory conditions might have pernicious effects. A. aquaticus and P. cavaticus showed a higher mortality using synthetic medium in situ and under laboratory conditions, which overestimated the stressor’s effect. Besides, substrate use had no significant effect. The novel acute in situ protocol allows the use of native species under realistic scenarios. It is particularly well adapted for assessing the risk of groundwater ecosystems but it can be applied to a wide range of ecosystems. View Full-Text
Keywords: groundwater ecology; groundwater ecotoxicology; stygofauna; in situ exposure; site-specific assessment groundwater ecology; groundwater ecotoxicology; stygofauna; in situ exposure; site-specific assessment
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MDPI and ACS Style

Castaño-Sánchez, A.; Malard, F.; Kalčíková, G.; Reboleira, A.S.P.S. Novel Protocol for Acute In Situ Ecotoxicity Test Using Native Crustaceans Applied to Groundwater Ecosystems. Water 2021, 13, 1132. https://doi.org/10.3390/w13081132

AMA Style

Castaño-Sánchez A, Malard F, Kalčíková G, Reboleira ASPS. Novel Protocol for Acute In Situ Ecotoxicity Test Using Native Crustaceans Applied to Groundwater Ecosystems. Water. 2021; 13(8):1132. https://doi.org/10.3390/w13081132

Chicago/Turabian Style

Castaño-Sánchez, Andrea; Malard, Florian; Kalčíková, Gabriela; Reboleira, Ana S.P.S. 2021. "Novel Protocol for Acute In Situ Ecotoxicity Test Using Native Crustaceans Applied to Groundwater Ecosystems" Water 13, no. 8: 1132. https://doi.org/10.3390/w13081132

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