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Towards the Optimization of eDNA/eRNA Sampling Technologies for Marine Biosecurity Surveillance

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Moss Landing Marine Laboratories, 8272 Moss Landing Road, Moss Landing, CA 95039, USA
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Coastal and Freshwater Group, Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand
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Institute of Marine Science, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand
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Department of Anatomy, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand
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Queenscliff Marine Research Facility, School of Life and Environmental Sciences, Deakin University, Queenscliff 3225, Australia
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Author to whom correspondence should be addressed.
Academic Editor: Lorenzo Mari
Water 2021, 13(8), 1113; https://doi.org/10.3390/w13081113
Received: 25 November 2020 / Revised: 21 March 2021 / Accepted: 10 April 2021 / Published: 18 April 2021
(This article belongs to the Special Issue Applications of Environmental DNA and RNA in Aquatic Ecosystems)
The field of eDNA is growing exponentially in response to the need for detecting rare and invasive species for management and conservation decisions. Developing technologies and standard protocols within the biosecurity sector must address myriad challenges associated with marine environments, including salinity, temperature, advective and deposition processes, hydrochemistry and pH, and contaminating agents. These approaches must also provide a robust framework that meets the need for biosecurity management decisions regarding threats to human health, environmental resources, and economic interests, especially in areas with limited clean-laboratory resources and experienced personnel. This contribution aims to facilitate dialogue and innovation within this sector by reviewing current approaches for sample collection, post-sampling capture and concentration of eDNA, preservation, and extraction, all through a biosecurity monitoring lens. View Full-Text
Keywords: eDNA; eRNA; marine biosecurity; invasive species eDNA; eRNA; marine biosecurity; invasive species
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MDPI and ACS Style

Bowers, H.A.; Pochon, X.; von Ammon, U.; Gemmell, N.; Stanton, J.-A.L.; Jeunen, G.-J.; Sherman, C.D.H.; Zaiko, A. Towards the Optimization of eDNA/eRNA Sampling Technologies for Marine Biosecurity Surveillance. Water 2021, 13, 1113. https://doi.org/10.3390/w13081113

AMA Style

Bowers HA, Pochon X, von Ammon U, Gemmell N, Stanton J-AL, Jeunen G-J, Sherman CDH, Zaiko A. Towards the Optimization of eDNA/eRNA Sampling Technologies for Marine Biosecurity Surveillance. Water. 2021; 13(8):1113. https://doi.org/10.3390/w13081113

Chicago/Turabian Style

Bowers, Holly A., Xavier Pochon, Ulla von Ammon, Neil Gemmell, Jo-Ann L. Stanton, Gert-Jan Jeunen, Craig D.H. Sherman, and Anastasija Zaiko. 2021. "Towards the Optimization of eDNA/eRNA Sampling Technologies for Marine Biosecurity Surveillance" Water 13, no. 8: 1113. https://doi.org/10.3390/w13081113

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