Phosphorus (P) and nitrogen (N) eutrophication of aquatic ecosystems and a general deterioration in water quality have increased with human population growth and agricultural development within many watersheds, and sources of watershed nutrient loading include both domestic and agricultural effluents [1
]. Such activities create negative impacts in natural wetland habitats by changing vegetation, soil biogeochemistry, habitat fragmentation, release of greenhouse gases, and severe vegetative overgrowth, resulting in deteriorating wildlife habitats. At our Laguna Cartagena study site, for example, severe cattail overgrowth and extensive peat formation due to phosphorus loading have reduced open surface conditions to as little as 10% (90% cover) and produced poorly oxygenated water [1
Excessive nutrient enrichment of P and N to aquatic ecosystems also leads to: excessive growth of nuisance algae, cyanobacteria, and other aquatic plants; high decomposition rates of the accumulated plant biomass that increase the production of organic matter and affect the biogeochemical cycles of N and P; water acidification; decreases in dissolved oxygen (anoxia and hypoxia); and decreases in biological diversity [1
Phosphorus in aquatic ecosystems occurs in both particulate and soluble compartments, in inorganic phosphate (PO4−3
) or bound in organic molecules [3
]. Soluble reactive phosphorus (SRP, o- PO4−3
, inorganic phosphorus) is the most readily bioavailable P fraction [10
]. Total phosphorus (TP) concentrations of 30–100 µg/L (ppb) and ˃100 µg/L (ppb), are considered eutrophic and hypereutrophic, respectively, for freshwater lakes [11
]. Frequently, the reduction of P loading into aquatic ecosystems has not resulted in reduced eutrophication [7
]. This is because P tends to accumulate in soil via lithification [15
] and subsequent disturbance and mobilization of P-enriched sediments (and possibly organic matter) can cause internal P eutrophication to the water column [16
P Sink dynamics are defined as occurring when inflow (inlet) P concentrations exceed outflow (outlet) concentrations (the lagoon absorbs P and sends water of lower P concentration downstream to other aquatic environments) [3
]. Conversely, P Source dynamics (or internal P loading) occur when outlet P concentrations exceed inlet concentrations (the lagoon releases P and sends water of higher P concentration downstream to other aquatic environments) [3
]. Internal P loading can occur from microbial-driven P release and release of inorganic P from mineral binding sites, especially under anaerobic conditions. These processes are controlled by oxygen concentrations, redox conditions, pH, temperature, and other drivers influenced by changes in hydrology [19
Hydrological and hydroperiod regimes can directly modify and change the physicochemical environment, stimulate microbial activity, and influence nutrient retention (sink) and release (source) processes. These regimes also transform wetland soils (sediments and organic matter) [3
] and cause eutrophication [7
] as sediments and organic matter provide internal sources of nutrients to the water column. At Laguna Cartagena this has resulted in a proliferation of emergent vegetation and, most notably, extensive floating peat mats (islands) and associated floating vegetation. This emergent vegetation covers ca. 90% of the lagoon’s surface, blocking light penetration and thus prohibiting the growth and photosynthesis of submerged plants, microalgae, and cyanobacteria. The resulting low dissolved oxygen concentrations negatively impact fisheries, and the overgrowth of floating peat islands reduce the available habitat for waterfowl that require open surface conditions.
Symptoms of severe problems of eutrophication in this shallow lagoon (Laguna Cartagena) have been observed since the 1950s, when most of the land area that drains to the lagoon was used for sugar cane cultivation. This formally oligotrophic freshwater wetland was thus affected by very intense phosphorus loading from inorganic agricultural fertilizer until the end of sugar cane cultivation and subsidized fertilizer use in the late 1990s. A legacy of this fertilizer use may remain in the soils but has not been adequately quantified.
In April 2012, the US Fish and Wildlife Service (USFWS) began a conservation plan that emphasized wildlife diversity, focusing especially on providing habitat for water birds and fish, and thus requiring an open water area of about 70% (30% cover), and well-oxygenated water [21
]. Hoping to achieve these goals, the agency engaged in management interventions that included the lowering of water levels, completely draining the lagoon by the end of May 2012, then excavating and dredging bottom sediments and removing floating peat mats. The lagoon was subsequently refilled in September of 2012. The lagoon was drained again during July 2013. On 21 August 2013, a prescribed burn of cattail-dominated habitat was conducted at Laguna Cartagena as part of a federal government training exercise for federal firefighters and other emergency personnel (as stated in the press). Refilling of the lagoon commenced immediately after the burn, yet within four months (December 2013) the emergent cattails and floating peat mats had returned to their previous extents.
Given the above events, and knowing beforehand the planning of the management interventions, the primary objective of this study was to assess the influence of these management interventions on phosphorus concentrations of the water released by the lagoon downstream in relation to the water that enters the lagoon, by characterizing soluble reactive phosphorus (SRP) and total phosphorus (TP) concentrations entering and leaving the lagoon prior to interventions (Phase One, September 2010 to September 2011) and after interventions (Phase Two, October 2013 to November 2014). A second objective was to determine the role of precipitations and water level changes on P sink and source dynamics.
2. Materials and Methods
2.1. Description of the Study Site
Laguna Cartagena is situated in the Lajas Valley, Municipality of Lajas, in southwestern Puerto Rico (Figure 1
). During the 1950s, the Principal Drainage Canal of the Lajas Valley Irrigation System, adjacent to the town of Magüayo, was connected through Laguna Cartagena. This canal carried agricultural effluent to the lagoon, and the water passed through the lagoon and subsequently flowed westward from the lagoon via an outlet canal (Figure 1
) to the wetlands and estuarine areas of the Boquerón State Bird Refuge, and then to the Caribbean Sea.
In 1989, Laguna Cartagena was acquired by US Fish and Wildlife Service (USFWS) and established as Laguna Cartagena National Wildlife Refuge (LCNWR). LCNWR originally was an open-water system and was noted for its abundance of wildlife, including large numbers of individual nesting and migratory waterbirds. Laguna Cartagena National Wildlife Refuge includes this tropical freshwater wetland and associated shallow lake, with a water depth of 0 to 2 m, corresponding to the 9 m and 11 m surface contours on the USGS (US Geological Survey) topographical maps (Figure 1
). The depth varies depending on precipitation and most importantly, on water level management by USFWS via the installation and removal of flashboards in the outlet weir.
During 1995 to 1998, SRP (orthophosphate or inorganic P, SRP) concentrations in the lagoon waters ranged to above 1000 and 1500 µg/L [22
]. Severe overgrowth of cattails (Typha domingensis)
and extensive floating peat mats and floating vegetation still occupy large portions of the available volume and surface area of the lagoon, altering the habitat and thus degrading the abundance and diversity of resident and migratory aquatic birds and fish. The lagoon exhibits other aquatic macrophytes including floating forms such as Pistia stratiotes
(water lettuce) and Eichhornia crassipies
(water hyacinth) that together with the floating peat mats cover 80–90% of the lagoon surface.
2.2. Sampling and Processing
Water samples were collected in triplicate at mid-column depths from the lagoon’s single inlet and the lagoon’s single outlet to characterize average total P and SRP during the annual cycle. All of the nutrients that enter the lagoon from this half of the drainage basin come together and enter through the combined inlet canal. Sampling occurred at two-week intervals from September 2010 through September 2011 (Phase One of this study) [23
]), prior to management interventions and from October 2013 through November 2014 (Phase Two of this study) [24
]), after management interventions.
All samples for P (SRP and TP) analyses were collected in glass bottles, preserved with H2
and transported in ice to the Universidad del Turabo (now Ana G. Méndez University, Gurabo). The samples were stored at a low temperature and analyzed within an interval of 1 week and a half after being collected using persulfate digestion and ascorbic acid methods [26
]. A Thermo Fisher Scientific, Genesys 20, with a wavelength range between 352 to 1100 nm was used to measure sample absorption at 880 nm.
Rainfall data were obtained weekly from a rain gauge located approximately 6 km north of the lagoon in the upper portion of the watershed, representative of regional rainfall. Daily rainfall data were recorded from August 2010 to September 2011 and September 2013 to November 2014. Stage (water level) data were collected at the adjustable crest weir at the lagoon. Stage was recorded weekly, and for each sampling day between July 2010 to October 2011 and July 2013 to November 2014 to document water level fluctuations. The bottom of the lagoon (0% fill) lies at the 9 m contour above sea level while full stage (100% fill) occurs at the 11 m contour (USGS 7.5-min topographic map), for a potential maximum lagoon depth (100% fill and overflow) of 2 m. Water level is controlled by USFWS’s placement and removal of flashboards at the outlet control structure (a weir) and thus are largely independent of rainfall.
We also analyzed samples of consolidated bottom substrate and flocculence in the center of the lagoon and collected general water quality data such as dissolved oxygen (DO), pH, total dissolved solids (TDS), temperature, turbidity, and nitrogen.
Dissolved oxygen, pH, TDS, temperature, and turbidity were measured in the field for each sampling event. The instruments used for measurement of these parameters were the Hanna Instruments (HI 98186) for DO, AZ Instruments Corp, model 8685 for pH and temperature, an EC/TDS/°C Martini (EC 59) meter for TDS and a turbidity tube for turbidity. Nitrate (), nitrite (), and ammonia (NH3) were determined using a HACH DR900 colorimeter. Nitrate was measured using the cadmium reduction method 353.2, nitrite was measured with the USEPA diazotization method and ammonium using the salicylate method. The sediment and flocculence samples were collected in 9 April 2011 (Phase One) and 10 August 2014 (Phase Two). Samples were transported in bags and processed by the Central Analytical Laboratory of the University of Puerto Rico’s Agricultural Experimental Station.
2.3. Statistical Analyses
Mean SRP and TP values were calculated for each sample (triplicate sampling, n = 3 for each sample). Descriptive statistics are herein provided for both Phase One (2010–2011) and Phase Two (2013–2014). One-way ANOVA and paired t-tests (µ1 > µ2) were used to compare phosphorus concentrations between both phases (Phase One and Phase Two). A multiple regression model was used to determine the response of phosphorus (SRPOI and TPOI) to several predictors including water level fluctuations (water levels one week prior to water sampling) and rainfall (one or two weeks prior to water sampling) during both phases. To describe the differences between outlet and inlet P concentrations, SRPOI was defined as the subtraction of SRPOutlet − SRPInlet and TPOI was defined as the subtraction of TPOutlet − TPInlet.
This study was conducted under the authorization of Biosafety Committee (IBC) Protocol B03-017-13 of the Ana G Mendez University System Office of Compliance.
3.1. Rainfall Conditions
Monthly rainfall (mm) for the Laguna Cartagena drainage basin recorded from August 2010 to September 2011 (Figure 2
) and September 2013 to November 2014 (Figure 3
) indicated heavy rainfall during the months of August to November and April to May, and allowed identification of specific rainfall events that occurred one and two weeks prior to water sample collection.
3.2. Stage Fluctuations
The maximum depth of 2 m (100% fill and overflow) at Laguna Cartagena was obtained during Phase One on the following dates: 5 October, 9 October, 7 November, 13 November, 29 November of 2010, 17 September, 24 September, and 2 October of 2011 (Figure 4
). Stage was controlled by USFWS by adjustment of the outlet weir crest and differed dramatically between Phase One (Figure 4
a) and Phase Two (Figure 4
b) of this study, i.e., the agency removed all flashboards from the weir in order to bring the stage level to “0”. Such events included periods of complete draining during summers of 2013 and 2014 (Phase Two) but no complete draining during the summers of 2010 and 2011 (Phase One).
3.3. Dissolved Oxygen, pH, Total Dissolved Solids, Temperature, Turbidity, and Nitrogen
Overall, Laguna Cartagena had a neutral pH (6.9–7.2) and temperatures that ranged from 25.5 °C to 27.6 °C (Table 1
). During Phase Two, DO increased for both the inlet (from 0.34 mg/L to 1.79 mg/L) and lagoon’s outlet (from 0.43 mg/L to 3.59 mg/L) (Table 1
). The overall DO concentration for 2013–2014 (Phase Two) was significantly greater than for 2010–2011 (Phase One) (Figure 5
). During both Phase One (33.6 cm) and Phase Two (29.5 cm), the lagoon’s outlet had a higher turbidity than the inlet (Table 1
Overall, during both Phase One (2010–2011) and Phase Two (2013–2014), nitrate concentrations were at or near zero (Figure 6
). Nitrite and ammonia concentrations for 2010–2011 (Phase One) were significantly greater than for Phase Two.
3.4. Analysis from Consolidated Bottom Substrate and Flocculence Associated with Floating Islands
Consolidated bottom substrate and the flocculence associated with floating islands in the center of the lagoon were acidic and with very high nutrient concentrations for both Phase One and Phase Two of this study (Table 2
). For Phase One, the pH for consolidated bottom substrate was typically about 4.16 and it was 3.86 for Phase Two. During 2014 the pH for flocculence in the center cattails was higher (4.30) than during 2011 (2.82).
For 2011 (Phase I) the Pavailable in the consolidated bottom substrate was 7750 µg/L but by 2014 (Phase Two) the available phosphorus (Pavailable) had increased (35,000 µg/L) in the center cattails. In 2013–2014 (Phase Two) the Pavailable (50,720 µg/L) for flocculence was 3-times less than those concentrations in 2011 (Phase One) (13,000 µg/L). By 2014, the %Total Kjeldahl Phosphorus (TKP, the combination of orthophosphates, hydrolyzable phosphorus and organic phosphorus) (0.17%) and %Total Kjeldahl Nitrogen (TKN, the total concentration of organic nitrogen and ammonia) (0.54%) values for consolidated bottom substrate were higher than the 2011 (Phase One) values of 0.09%TKP and 0.39%TKN. By 2014, %TKP in the flocculence decreased to 0.08% and the %TKN increased to 0.98%. During 2014 (Phase Two), nitrate () values decreased to non-detectable values for both the consolidated bottom substrate and for flocculence. In 2011, the for consolidated bottom substrate was 34,000 µg/L and 1000 µg/L for flocculence. Nitrite () was not detectable in neither the consolidated bottom substrate nor in the flocculence for both Phase One and Phase Two of study. In 2011 (Phase One), ammonium in the consolidated bottom substrate was 220,000 µg/L, decreasing to 80,0000 µg/L in 2014 (Phase Two). The NH3 concentrations for flocculence increased from 63,000 µg/L (2010) to 163,000 µg/L (2014).
3.5. Phosphorus Source/Sink Dynamics
data from 2010–2011 (Phase One) and 2013–2014 (Phase Two) at Laguna Cartagena (Figure 7
) exhibited relatively minor P release (source) events during 2010–2011 in contrast to dramatic P export events during 2013–2014. Laguna Cartagena was a significant source of SRP downstream on 18 October, 20 November of 2010 with lesser releases on 29 December of 2010 and 9 April of 2011. The lagoon was a source (release) of TP on six occasions (5 October, 18 October, 20 November, 6 December, 29 December of 2010, and 28 May of 2011) with negligible release on 7 November of 2010. During Phase Two, the lagoon was a source of SRP on 4 January, 15 March, 30 March, 17 April, 3 May, 31 May, and 21 June of 2014 (Figure 7
) on 7 of 17 sample periods, and source of TP on 11 of the 17 samplings (5 December of 2013, 4 January, 26 January, 23 February, 15 March, 30 March, 17 April, 3 May, 31 May, 21 June, and 21 September of 2014; Figure 7
3.6. Source/Sink Dynamics under High Versus Low Rainfall and Water Level Fluctuations
During Phase One of this study (pre intervention, 2010–2011), stage fluctuations (water level (WL) one-week prior to sampling) and rainfall (one week (1R) and two weeks (2R) prior to sampling) affected the source–sink dynamics. The data had a normal distribution and the results of the regression equations were, for SRPOI: −190 + 104 (WL) − 0.614 (1R) + 0.734 (2R) (r2 = 59.4%, p = 0.007) and for TPOI: −736 + 440 (WL) − 0.18 (1R) + 3.23 (2R) (r2 = 55.9%, p = 0.012).
Therefore, both SRP and TP dynamics were functions of stage fluctuations one week prior to sample collection and rainfall both one and two weeks prior to sample collection, indicating the effect of stage and rainfall, with a lag.
During Phase Two (post intervention, 2013–2014), the data also had a normal distribution and the regression equations were, for SRPOI: 130 − 21 (WL) − 7.84 (1R) + 3.64 (2R) (r2 = 22.3%, p = 0.368) and for TPOI: −769 + 1097 (WL) + 18.3 (1R) − 20.1 (2R) (r2 = 12.7%, p = 0.637). Thus, SRP and TP dynamics were not significant functions of stage fluctuations one week prior to sample collection, nor functions of rainfall (neither one nor two weeks prior to sample collection).
3.7. Comparison of Phase One and Phase Two P Concentrations
During Phase One, at the inlet, the minimum values of SRP and TP were 85.4 µg/L and 525.9 µg/L; and the maximum values were 286.8 µg/L and 2117.5 µg/L, and varied with increasing rainfall (Figure 8
). SRP concentrations from lagoon’s outlet in Phase One, varied between 43.3 µg/L and 270.8 µg/L (Figure 9
). The minimum TP concentration here was 229.4 µg/L and the maximum value was 1471.7 µg/L (Figure 9
During Phase Two, at the combined inlet the minimum values of SRP and TP were 35.8 µg/L and 253.9 µg/L; and the maximum values were 574.2 µg/L and 4665.3 µg/L (Figure 8
). SRP from lagoon’s outlet varied between 20.9 µg/L and 927.5 µg/L (Figure 9
). The minimum TP concentrations was 249.3 µg/L, and the maximum value was 4529.1 µg/L (Figure 9
Overall population means of Inlet SRP concentrations were 180.8 µg/L and 231.5 µg/L for 2010–2011 versus 2013–2014 (Figure 8
, Figure 10
) and were not significantly different (One-way ANOVA, p
= 0.417; Paired-t (µ1
= 0.776). Overall population mean concentrations of inlet TP, at 840.8 µg/L for 2010–2011 and 966.9 µg/L for 2013–2014 were not significantly different (One-way ANOVA, p
= 0.575; Paired-t (µ1
= 0.807) (Figure 8
, Figure 10
Although overall population means of outlet SRP concentrations of 127.7 µg/L and 269.8 for 2010–2011 versus 2013–2014 were not significantly different (One-way ANOVA, p
= 0.380; Paired-t (µ1
= 0.888), comparisons of similar sampling dates (Figure 9
and Figure 11
) show substantially higher concentrations of SRP in Phase Two.
Overall population mean concentrations of outlet TP, for 2013–2014 (1573.0 µg/L) were significantly greater than for 2010–2011 (703.1 µg/L) using one-way ANOVA (p
= 0.011) and paired t-tests (µ1
= 0.025) (Figure 11
). Moreover, comparisons of similar sampling dates (Figure 9
, Figure 11
) show dramatically higher concentrations of TP in Phase Two. Thus, while the phosphorus concentrations of inputs into the lagoon were similar in Phase One and Phase Two, the concentrations of phosphorus in water flowing out of the lagoon were notably higher in the post intervention Phase Two than prior to intervention (Phase One).
This study investigated the effects of intensified management interventions including excavation and removal of sediment and vegetation, draining, and burning on soluble reactive phosphorus (SRP) and total phosphorus (TP) concentrations of lagoon water, focusing on SRP and TP input and export during September 2010–September 2011 (Phase One) and October 2013–November 2014 (Phase Two), and including the roles of precipitation and water level changes on P sink and source dynamics. The results of water and soil/sediment analyses showed that Laguna Cartagena is a hypereutrophic and anoxic ecosystem that acted various times as both a sink and source of TP and SRP with internal phosphorus loading (internal eutrophication) from the lagoon’s own sediments, causing higher phosphorus concentrations in lagoon water, downstream to other ecosystems.
Release of SRP and TP at the lagoon’s outlet during Phase Two was substantially higher than during Phase One. We found no significant differences between P concentrations at the inlet canal for Phase One versus Phase Two. The lagoon also showed depleted N concentrations in the water column. During Phase One, P dynamics within the lagoon were primarily influenced by rainfall (one and two weeks prior sample collection) and water flows to the lagoon. In Phase Two the lagoon acted more often as a source of SRP and TP and this dynamic behavior was primarily influenced by internal biological and physiochemical processes within the lagoon. The USFWS Laguna Cartagena Conservation Plan emphasizes wildlife diversity and habitat restoration, but for this to be achieved the prevention and control of nonpoint P pollution is required, including the control of internal eutrophication. While nutrient and pollution inputs to the lagoon are beyond the control of the USFWS, the agency can nonetheless provide optimal wildlife habitats (keeping vegetative cover to about 30% instead of 90%) through the use of a common floating surface dredge. This would avoid disturbing bottom sediments, thus preventing excessive release of P to the lagoon water column and downstream to other water bodies and the Caribbean Sea. We recommend allowing normal (moderate) seasonal stage level fluctuations, but the complete cessation of draining, dredging, burning, excavations, and other interventions that can alter or disturb sediments.