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Article

Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli

1
Department of Biotechnology and Bioengineering, Center for Research and Advanced Studies of the National Polytechnic Institute, Mexico City 07360, Mexico
2
Department of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain
3
Department of Marine Biology and Oceanography, Institute of Marine Sciences (ICM-CSIC), 08003 Barcelona, Spain
4
Grupo de Ciencia e Ingeniería en Sistemas Ambientales, Facultad de Ingeniería Civil, Universidad del Cauca, Cauca 190001, Colombia
5
Sustainability and Health and Environmental Microbiology Laboratory, Universitat Politècnica de Catalunya-BarcelonaTech, Edifici Gaia, 08222 Terrassa, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Anna Barra Caracciolo
Water 2021, 13(19), 2731; https://doi.org/10.3390/w13192731
Received: 15 August 2021 / Revised: 14 September 2021 / Accepted: 28 September 2021 / Published: 2 October 2021
(This article belongs to the Special Issue Water and Human Settlements of the Future)
Cork matrices biofunctionalized with Moringa oleifera seed extracts (MoSe) have potential for use as a biofilter with antibacterial properties to reduce waterborne pathogens. The aim of this study was to evaluate the effect of cork biofunctionalized with active antimicrobial compounds of MoSe (f-cork) on the inhibition of Escherichia coli (InhEc). The LacZ gene from a strain of E. coli was used as the target sequence using viability quantification Polymerase Chain Reaction (qPCR) and differentiation of viable and dead bacteria through selective cell viability PMA staining. To perform this, a 27−4 fractional factorial design and a biofiltration system were used to evaluate the effect of the active protein in MoSe immobilized in granulated cork on InhEc. We found that the potential for antimicrobial activity increased with f-cork for an effective maximal bacterial reduction (99.99%; p < 0.05). The effect of f-cork functionalized with MoSe on E. coli viability was of 0.024% and 0.005% for the cells exposed to PMA, respectively, being the relevant conditions in treatment 2: (0 L/min) without aeration, (5%) MoSe and (5 mm) cork particle. In conclusion, the f-cork functionalized with MoSe presented biosorbent and antibacterial properties that effectively reduced the E. coli growth. View Full-Text
Keywords: cork-based biofilter; Escherichia coli; viability qPCR; Lac Z gene; propidium monoazide; disinfection; antimicrobial activity cork-based biofilter; Escherichia coli; viability qPCR; Lac Z gene; propidium monoazide; disinfection; antimicrobial activity
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MDPI and ACS Style

Infante, N.; Rodríguez, R.; Bartolo, Y.; Sánchez, O.; Sanz, I.; Bermeo, L.; Morató, J. Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli. Water 2021, 13, 2731. https://doi.org/10.3390/w13192731

AMA Style

Infante N, Rodríguez R, Bartolo Y, Sánchez O, Sanz I, Bermeo L, Morató J. Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli. Water. 2021; 13(19):2731. https://doi.org/10.3390/w13192731

Chicago/Turabian Style

Infante, Nury, Refugio Rodríguez, Yaneth Bartolo, Olga Sánchez, Isabel Sanz, Lizeth Bermeo, and Jordi Morató. 2021. "Biofunctionalization of Cork with Moringa oleifera Seeds and Use of PMA Staining and qPCR to Detect Viability of Escherichia coli" Water 13, no. 19: 2731. https://doi.org/10.3390/w13192731

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