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Residual Maintenance Using Sodium Hypochlorite, Sodium Dichloroisocyanurate, and Chlorine Dioxide in Laboratory Waters of Varying Turbidity

Department of Civil and Environmental Engineering, Tufts University, Medford, MA 02155, USA
Department of Disease Control, London School of Hygiene and Tropical Medicine, Keppel St, London, WC1E 7HT, UK
Author to whom correspondence should be addressed.
Water 2019, 11(6), 1309;
Submission received: 11 May 2019 / Revised: 15 June 2019 / Accepted: 19 June 2019 / Published: 25 June 2019
(This article belongs to the Special Issue Water, Sanitation, and Hygiene in Humanitarian Contexts)


Sodium hypochlorite (NaOCl) and sodium dichloroisocyanurate (NaDCC) are commonly used for household water treatment (HWT); chlorine dioxide (ClO2) is a potential new HWT option. We compared the residual maintenance of NaOCl, NaDCC, and ClO2 over 24 hours using recommended dosages (2 and 4 mg/L) in waters of varying turbidity (0–300 NTU, from kaolin clay or creek-bottom sediments) and total organic carbon (TOC) concentrations (0–100 mg/L), for a total of 324 reactors. NaOCl and NaDCC had similar free chlorine decay rates, and ClO2 decayed more rapidly across all of the tested conditions. Little variability was observed across clay-based turbidity levels and TOC concentrations. With a dosage of 2 mg/L, a residual ≥0.2 mg/L was maintained at 30 NTU for NaOCl and 100 NTU for NaDCC; for ClO2, 4 mg/L were required to maintain ≥0.2 mg/L under all conditions except at zero turbidity. Comparisons with data from the literature suggest that the three compounds would inactivate E. coli, rotavirus, and Giardia cysts within 1 hour under all conditions, except 300 NTU for NaOCl and NaDCC. All three disinfectants are similarly efficacious for this usage; however, differences are seen in decay rates that may influence disinfectant selection depending on water storage time.

1. Introduction

Worldwide, approximately 2.1 billion people lack access to a safely managed drinking water service, including 582 million people who use unimproved or surface water sources [1]. Household water treatment (HWT) can be a cost-effective means of improving drinking water quality [2] and reducing diarrheal disease in households where access to safe water is limited [3]. HWT is therefore recommended as part of a comprehensive strategy to prevent infections and to improve the health of vulnerable populations without access to safe drinking water in low-resource and emergency settings [4,5].
Chlorination is a common HWT method, and chlorination programs generally include three elements: Water treatment with a chlorine product, such as liquid sodium hypochlorite (NaOCl) or tablets of sodium dichloroisocyanurate (NaDCC) at the household level; the storage of treated water in a safe container; and a behavior change communication to improve water handling practices and hygiene [6]. In a meta-analysis of 21 health impact studies of household chlorination with sodium hypochlorite, the overall reduction in diarrheal disease averaged 29% in users and 41% in confirmed users [7]. Currently, bottles of liquid chlorine and chlorine tablets are widely marketed and distributed in development and emergency contexts [8,9,10].
A limitation of chlorination is the need to determine the appropriate dosage for each water source, which depends on the “chlorine demand” [11]. This chlorine demand is the amount of chlorine consumed by reactions with various organic and inorganic substances present in the water matrix, and is empirically determined for each source. In each water source, the chlorine dosage must be sufficient to account for this chlorine demand, before disinfection can occur. Chlorine demand is exerted by a set of compounds, including, but not limited to: Natural organic materials (e.g., phenols, amines, acids, aldehydes, ketones, pyrrole groups, and inorganics and metals (ammonia, arsenic, iron, manganese), and halides (including sulfide, bromide, cyanide, iodide)) [12,13,14]. These reactions can be instantaneous (e.g., with metals) or first- or second-order decay reactions over time. Additionally, biofilm growth, the material of pipes (or storage containers), exposure to ultraviolet (UV) from sunlight, pH, temperature and contact time all impact chlorine residual decay over time [12,15].
The concentration of natural organic material in water is typically expressed as total organic carbon (TOC), which is the sum of dissolved and particulate organic carbon. Natural organic material is dominated by the presence of humic substances, such as fulvic and humic acids. Several equations are empirically derived to describe chlorine demand kinetics as a function of total organic carbon (TOC) [12,14]. However it is cautioned that these relationships are specific to water matrix conditions, and are not representative of all waters.
Turbidity is often used as a rough indicator for chlorine demand and/or TOC, despite the limitations of this metric, because it is simple to measure [16]. In a study of 158 drinking water sources from 22 low-income countries, measured drinking water turbidity ranged from 0–551 nephelometric turbidity units (NTU) [16]. It was determined that an ~2 mg/L chlorine dosage for water from improved sources of <5 or <10 NTU turbidity met free chlorine residual (FCR) criteria of ≥0.2 mg/L 91–94% and 82–87% of the time after 8 and 24 hours of storage, respectively. In unimproved water source samples, a ~4 mg/L dosage met ≥0.2 mg/L 83% and 65% of the time after 8 and 24 hours, respectively. Based on these results, the Centers for Disease Control and Prevention (CDC, Atlanta, Georgia) and the World Health Organization (WHO) recommend to adapt chlorine dosage for HWT depending on turbidity (as an indicator for chlorine demand): 2 mg/L for waters with <10 NTU turbidity, and 4 mg/L at higher turbidity levels [16,17,18]. Direct chlorination is not recommended for waters with >100 NTU turbidity [16]. These dosage guidelines were developed specifically for HWT to ensure: 1) The inactivation of bacterial and viral pathogens present in the water; and, 2) the maintenance of an adequate FCR (≥0.2–0.5 mg/L) to prevent recontamination over 24 hours of household storage.
The dosage guidelines are applicable to both NaOCl and NaDCC. However, there is interest in using other disinfectants, particularly chlorine dioxide (ClO2), which has been commercialized for point-of-use water treatment by several companies in recent years [19,20,21,22]. NaOCl, NaDCC, and ClO2 have varying chemical characteristics (Table 1).
NaOCl is a hypochlorite salt soluble in water and has been used for drinking water disinfection since the early 1900s [12]. In water, NaOCl releases hypochlorous acid (HOCl), which exerts a biocidal activity [12].
NaOCl solutions (commonly termed “bleach”) can be produced locally or regionally by passing gaseous chlorine into a stream of water, by diluting powdered chlorine, or by the electrolysis of salt and water [23]. Sodium hydroxide is added to ensure a 12–18 month shelf-life by raising the pH (towards a higher alkalinity) [24]. The use of NaOCl in the presence of organic compounds can lead to disinfectant by-product formation, including trihalomethane (THM) formation, long-term ingestion of which can increase cancer risk [25]. If CDC- and WHO-recommended dosages are used, the THM concentrations remain below WHO guideline values, even at high turbidity levels [26]. In low-resource settings, NaOCl solutions are usually packaged in bottles with instructions to add one full bottle cap of the solution to clear water (or two caps to turbid water) in a standard sized storage container, then to agitate and afterwards wait 30 minutes before drinking [6].
NaDCC is industrially produced as a white crystalline powder, which can be tabletized, with a shelf-life of 3–5 years [8,27]. While both NaOCl and NaDCC release HOCl as biocidal compound for disinfection, the release mechanism is slightly different for NaDCC, as free chlorine release from NaDCC is mediated by cyanurate molecules [28]. NaDCC use also leads to THM formation, although if recommended dosages are used, these THM concentrations remain below WHO guideline values [29]. In low-resource settings, NaDCC is typically packaged in strips or bottles, and its users are instructed to add one tablet to clear water (or two to turbid water), to agitate, and then wait 30 minutes before drinking [6].
ClO2 is a gas at room temperature, and exists almost entirely as monomeric free radicals, with a relatively constant efficacy over a wider pH range (6–10) than chlorine [12,30]. ClO2 use has increased in recent decades due to lower THM formation potential, higher efficacy in treating viruses, and stronger oxidation power than sodium hypochlorite [30]. Recently, ClO2 has been promoted for HWT use, and packaged as a liquid [19] or in the form of tablets [20]. To our knowledge, the appropriate dosage and residual maintenance for ClO2 use for HWT has not been evaluated.
Thus, a knowledge gap exists about the relative residual maintenance over 24 hours—to ensure safety during household water storage—of the three disinfectants. The majority of research to date on FCR maintenance is with NaOCl, with some conducted on NaDCC [16,31]. There is a common perception that NaDCC maintains higher FCR over time than NaOCl (due to the mediation by cyanurate molecules), particularly in turbid waters. However, there is only limited evidence to support this at drinking water concentrations, and studies highlight that more research is needed at higher turbidity levels [16,31].
The goal of the research presented herein is to compare residual maintenance over 24 hours using recommended dosages for NaOCl, NaDCC, and ClO2 in laboratory-controlled waters with varying turbidity and added TOC levels to inform recommendations for HWT.

2. Materials and Methods

From June to December 2016, we prepared 10-L reactors with two types of added turbidity at five different turbidity levels and three added TOC concentrations, then treated these with one of three disinfectants at the CDC/WHO recommended dosage (Figure 1), and measured the disinfectant residual at 1, 2, 4, 8, and 24 hours after disinfectant addition. As there was no difference in the turbidity type at 0 NTU, 162 conditions were tested in duplicate, for a total of 324 reactors.

2.1. Turbidity

To represent various turbidity types seen in surface water used for drinking in low-resource settings, reactors were spiked with laboratory-grade white kaolin clay (Lion China Clay USP, Charles B. Chrystal Co., Larchmont, NY, US) or creek bottom sediments collected from the Mystic River (Medford, MA, US). The 0, 10, 30, 100, and 300 NTU targets were set to be consistent with previous research and with chlorine dose breakpoints [16,32,33]. Pre-testing was conducted to determine the amount of clay/sediments required to obtain turbidity targets in reactors using a calibrated Hach 2100 portable turbidimeter (Loveland, CO, US).
Twenty-four hours before each testing day, creek bottom sediments were collected from a consistent location on the Mystic River. Using a plastic scoop, one inch of the soil layer where the river met the bank was removed to eliminate any rocks. The softer soil below was then scooped into a gallon-sized Ziploc™ bag (Racine, WI, US) and transported to the laboratory. On each testing day, clay or sediments were added, and the turbidity was confirmed to be within 10% of the target. If any results were outside this range, the reactor was recreated.

2.2. TOC

To obtain the desired added TOC concentration, an appropriate amount of 10,000 mg/L TOC stock solution comprised of 1,2 bensenedicarboxylic acid, monopotassium salt (2.11% wt) and phosphoric acid (0.27% wt) was pipetted into each reactor (Hach, Loveland, CO, USA) [34]. The 0, 10, and 100 mg/L concentrations were selected to encompass the range of naturally occurring waters [35]. After addition, each opaque reactor was covered with cardboard to protect the TOC molecules from light.

2.3. Disinfectants and Dose

The CDC/WHO recommended dosages of 2 and 4 mg/L for clear and turbid water, respectively, were tested for each disinfectant under all turbidity and TOC conditions. To determine the volume of the NaOCl solution (“bleach”) to add to reactors, the Hach digital titration method 8209 (Loveland, CO, USA) was used to measure the total chlorine concentration in a laboratory-grade NaOCl solution (Val Tech, Zelienople, PA, US) and the dilution factors were calculated. For NaDCC, the number of non-expired Aquatabs™-brand tablets (Medentech, Ireland) to add to the reactors was calculated, and the dosages were confirmed by measuring the total chlorine residual five minutes after addition to chlorine demand-free water, using a calibrated LaMotte 1200 colorimeter with DPD-1 and DPD-3 (N,N-diethylparaphenylene diamine) tablets (Chestertown, MD, USA). Pre-testing was conducted with chlorine demand-free water to determine the appropriate volume of liquid ClO2 product (Xinix AB, Rosersberg, Sweden) to add to the reactors in order to achieve target dosages. The determined dosages were added and confirmed using the colorimeter with DPD-1.

2.4. Reactor Testing

Immediately before disinfectant addition, the temperature and pH in each reactor were measured using a thermometer and a calibrated Hanna multi-meter (Bedfordshire, UK). After disinfectant addition, FCR or ClO2 were measured at 0, 1, 2, 4, 8, and 24 hours using the LaMotte colorimeter following methods approved by the United States Environmental Protection Agency (EPA) for disinfectant residual testing, namely the Standard Method 4500-Cl-G for FCR, and 4500-ClO2-D for ClO2 [36,37]. Please note that for ClO2 measurements, we did not use glycine as recommended in Standard Method 4500-ClO2-D, because this is intended to neutralize free chlorine, which was not present in the MilliQ water used in our experiments. Standard Methods include zeroing the colorimeter with the water sample as a blank before adding the DPD-1 to minimize turbidity/TOC interference in the test.
Measurements were reported in mg/L at two significant digits, as determined to be the accuracy and precision of the meter in previous research [38]. Temperature, pH, and turbidity were measured 24 hours after addition (as described above). All meters were calibrated daily.

2.5. Data Analysis

All data were entered and graphs prepared in Microsoft Excel (Redmond, WA, US). Statistical analyses were run in STATA 14.1 (StataCorp LP, College Station, TX, US). FCR and ClO2 decays were evaluated by fitting the mean of the duplicate measurements obtained at 0, 1, 2, 4, 8, and 24 hours for each disinfectant, dosage, turbidity type, turbidity level, and added TOC concentration to a first-order model.
Three different metrics were then used to assess the results: 1) Whether decay rates differed between tested compounds; 2) whether measured residual concentrations exceeded the WHO/CDC-recommended minimum for FCR of 0.2 mg/L after 24 hours; and, 3) whether the dose and contact time (CT-factor) were sufficient to inactivate representative bacteria, virus, and protozoa. Each of these metrics is further described below.
First-order decay constants k were determined by fitting the following first-order decay equation for each dosage, turbidity type, and turbidity level:
l n ( C t C 0 ) = k t
where Ct is the FCR concentration at time t (in hours), and C0 the initial FCR concentration.
Disinfectant decay rates were compared using an analysis of covariance (ANCOVA) with both time and the natural log of residual concentrations as covariates. Tukey’s post-hoc tests were used to determine which disinfectants differed, if any.
For each dosage, type, and turbidity, the measured residual after 24 hours was compared to the CDC/WHO guideline target of a minimum of 0.2 mg/L. A particular point of investigation was assessing “policy-relevant” differences, where one disinfectant met the minimum concentration and another did not.
Lastly, to compare disinfectant efficacy in disinfecting water, we calculated the CT-factors. The CT-factor is the multiple of the disinfectant concentration and the exposure time, and quantifies the contact time and the dose needed to inactivate the organisms [39]. CT-factors were calculated for the 1-hour and 24-hour time points for all of the tested combinations of turbidity and dosage by multiplying the measured FCR or chlorine dioxide residual concentration at each point by 60 minutes (for the 1-hour time point) or 1,440 minutes (for the 24-hour time point), respectively. Please note this is a conservative calculation, as the residual concentration at the end time point was used for the whole time.
These results were compared to known CT-factors for selected bacteria (Escherichia coli), virus (human rotavirus), and protozoa (Giardia lamblia). We chose E. coli because it is the standard bacteria to use for water quality analysis [17], human rotavirus because it is the etiologic agent most responsible for childhood diarrhea in developing countries [40], and Giardia cysts because they are common, and chlorination is not effective against any of the Cryptosporidium spp. [39].

3. Results

In total, 324 reactors were tested (as shown in Figure 1 in Methods). All reactors met the desired criteria for turbidity on first creation, except for three (0.9%), which were prepared again.

3.1. Free Chlorine and Chlorine Dioxide Residual Decay

Free chlorine decays over time in all of the samples (Figure 2). There is little variation between samples with different added TOC concentrations and different turbidities created with clay (Figure 3, Figure S1). As such, samples with creek bottom sediments at the added TOC concentration 10 mg/L are presented in Figure 2 (please note all data and results are available from the authors). As can be seen, NaOCl and NaDCC decay curves appear similar, with increasing decay at increasing turbidities, and chlorine dioxide decays more rapidly across all turbidities.
First-order decay constants k are calculated from duplicates across the three added TOC levels (n = 6) for each dosage and turbidity condition (Table 2). Significant differences between disinfectants (p ≤ 0.0004) are detected for all dosage and turbidity conditions by ANCOVA. Post-hoc test results indicate that ClO2 decay rates are significantly different from those of NaOCl and NaDCC under all but two cases (for both dosages at a turbidity of 100 NTU from creek-bottom sediments). NaOCl and NaDCC decay rates are statistically significantly different under 5 out of 18 cases, at both dosages with 30 and 100 NTU sediment and at 4 mg/L dosage with 300 NTU sediment. All p-values from the post-hoc test are reported in Table S1.

3.2. Meeting Minimim Standards

For NaOCl and NaDCC, the mean FCR concentrations after 24 hours are ≥0.2 mg/L in all reactors without added turbidity and with clay-based turbidity (Table 3). Residuals are <0.2 mg/L in all reactors with sediments at 300 NTU, and for three of the four conditions at 100 NTU (at both dosages for NaOCl, at the low dosage only for NaDCC).
For ClO2, 7 of 9 cases at 2 mg/L dosage have <0.2 mg/L, the two remaining cases (no turbidity and 300 NTU clay) have <0.3 mg/L. At 4 mg/L dosage, 8 of 9 cases are ≥0.2 mg/L, with the exception of the 300 NTU sediment. Upon observation, ClO2 is more consistent across each turbidity type and level than NaOCl and NaDCC.

3.3. CT-factor Analysis

CT-factors are found in the literature for free chlorine and ClO2 for the inactivation of E. coli, human rotavirus, and Giardia cysts (Table 4). CT-factors for the selected organisms cannot be retrieved from the three identified studies evaluating the microbial efficacy of NaDCC for water treatment [42,43,44]. While NaDCC and NaOCl have different chemistries and HOCl release mechanisms, it is postulated that available data on the efficacy of free chlorine for water disinfection is relevant to NaDCC, as both NaOCl and NaDCC release HOCl; however this is unconfirmed [27].
For NaOCl, CT-factors at 1 hour range from 6.0–109.3 and 25.3–224.9 mg-min/L, at 2 and 4 mg/L, respectively (Table S2). For NaDCC, CT-factors at 1 hour range from 9.3–118.5 and 61.8–244.6 mg-min/L, at 2 and 4 mg/L, respectively. For ClO2, CT-factors at 1 hour range from 41.5–62.4 and 100.2–127.0 mg-min/L, at 2 and 4 mg/L, respectively. Across all three compounds, the lowest CT-factors are at the 300 NTU sediment and 2 mg/L dosage, the highest values are in the 4 mg/L dosage with clay (NaDCC, NaOCl) or no turbidity (ClO2).
CT-factors for the 1-hour time point for the three compounds are higher than the values reported to inactivate E. coli and the human rotavirus under all tested conditions (Table 4, Table S1). However, Giardia cysts have the highest CT-factor of the three selected organisms. Assuming a 9 mg-min/L CT-factor necessary to inactivate Giardia, the only test condition that do not meet a 2-log inactivation is a 2 mg/L dosage of NaOCl in 300 NTU sediments. Assuming a 12 mg-min/L CT-factor necessary to inactivate Giardia, the only test conditions that do not meet a 2-log inactivation are 2 mg/L dosages of NaOCl or NaDCC in 300 NTU sediments.

4. Discussion

We systematically assessed the impact of disinfectant, dosage, turbidity level, turbidity type, and added TOC concentration on residual maintenance and disinfection efficacy in the laboratory setting; testing a total of 324 reactors. We found that NaOCl and NaDCC had similar decay curves, while ClO2 was depleted more rapidly, which can be attributed to the volatile and gaseous nature of ClO2. Over 24 hours, NaOCl and NaDCC maintained adequate FCR (≥0.2 mg/L) up to high sediment-based turbidity (30–100 NTU) with both initial dosages of 2 mg/L and 4 mg/L. In contrast, ClO2 residuals were ≥0.2 mg/L for only two of the nine conditions with an initial dosage of 2 mg/L, and for eight out of nine conditions at a higher dosage (4 mg/L). In terms of efficacy, these results suggest that the three tested disinfectants would inactivate the three WHO Evaluation Scheme pathogens within one hour under all of the tested conditions, except the highest sediment turbidity (300 NTU) for NaOCl and NaDCC. These results are generally concurrent with previous literature and informed practice, and suggest that NaOCl, NaDCC, and ClO2 are equally efficacious in terms of pathogen removal for HWT applications. However, NaOCl and NaDCC achieve higher residual maintenance, which is important to protect water from recontamination during storage in households. Our results inform the literature, particularly on the topics of: 1) The balance between disinfectant, dose, residual maintenance, and efficacy in HWT; and, 2) appropriate compounds to add in the laboratory setting to model natural waters.
While NaOCl and NaDCC retain similar FCR throughout the majority of reactors tested, there are slight differences at 24 hours for turbidity from sediments: 1) At 30 and 100 NTU, the first order decay constants are statistically different at both dosages, however at 300 NTU they are only different at 4 mg/L; 2) at 100 NTU, a difference is seen between the number of samples ≥0.2 mg/L, but please note that this difference is not seen at 30 or 300 NTU. Taken together, these results indicate that the residual is slightly better maintained with NaDCC between 30–100 NTU. However, this only becomes practically and policy-relevant at 100 NTU, when fewer NaOCl results are ≥0.2 mg/L compared to NaDCC. By 300 NTU, the results converge again, as both chlorine compounds do not maintain FCR ≥ 0.2 mg/L. These results are similar to results from 43 samples collected in Tanzania [31]. Thus, there is a question of how meaningful the slight statistical difference is in NaOCl and NaDCC between 30–100 NTU. Overall, these results support the current WHO and CDC recommendations to use 2 mg/L of NaDCC or NaOCl for waters of turbidity 0–10 NTU, 4 mg/L of NaDCC or NaOCl for waters of turbidity 10–100 NTU, and not to use chlorination alone at turbidities >100 NTU (as with these dosages a ≥0.2 mg/L is maintained over 24 hours) [16]. The decision whether to use NaDCC or NaOCl for water treatment will depend upon the context, including availability, cost, and user acceptance factors. Generally, NaDCC has the benefits of a longer shelf life (three years), but the drawbacks of a higher unit cost; while NaOCl has the benefits of a lower unit cost, but drawbacks of shorter shelf-life (12 months). Of note is that for high turbidity water, users should ideally be using a multi-barrier method for household water treatment, such as filtration or flocculation, followed by disinfection (e.g., chlorination) [6].
ClO2 has historically been used primarily for centralized water treatment due to its gaseous nature and instability, as it is made of free radicals and reacts quickly to treat water. ClO2 solutions have to be kept cool, sealed, and protected from light to avoid disproportionation [50], and this is difficult to ensure in field conditions relevant to HWT. Results from this study indicate that ClO2 decays more rapidly than NaOCl or NaDCC, and may therefore not provide adequate protection from recontamination during water storage in households. However, new products have been released on the market, including ClO2 tablets [20], that offer promising perspectives for HWT applications and warrant further evaluations.
We tested three mechanisms to add chlorine demand to our test waters: Kaolin clay, added TOC standard, and creek-bed sediments. These mechanisms were selected to model the different types of chlorine demand producing compounds seen in field waters (clay from rivers, TOC from natural organic materials, and creek-bed sediments from river bottoms) [51]. However, as is evident in the study, two of these compounds appear to add little chlorine demand. As kaolin clay is purely mineral (Al2H4O9Si2), it may not add chlorine demand to water.
There are different TOC standards available for laboratory settings, and we made an assumption that they would all have a chlorine demand. The TOC standard we used was produced from a dicarboxylic acid, monopotassium salt and phosphoric acid [34], which had limited chlorine demand.
We are not the first to make this assumption. The WHO and National Science Foundation (NSF) developed a testing protocol for evaluating HWT technologies against prepared test waters. The protocol calls for the use of tannic acid in general (low TOC) test waters and humic acid in challenge (high TOC) test waters [49]. The addition of tannic or humic acid is based on the weight of carbon in each compound. However, while this ensures that the additional organic carbon load is consistent, it does not account for any differences in chlorine demand exerted by varied TOC standards, including the different acids, as each acid is comprised of different organic materials (tannic acid is a polyphenol and humic acid has both phenolic and carboxylic substitutes). There were challenges in testing chlorine-based HWT products noted in the WHO Evaluation Scheme, and we are working in consultancy with WHO to determine ways to ensure that TOC additives to general and challenge test waters do not have controlled carbon addition, but instead that they have controlled chlorine demand addition, which is an accurate way to assess chlorine-based HWT products. Further research into accurate test waters with controlled chlorine demand to assess chlorine product efficacy is needed. We are currently working on this in our laboratory.
There are several limitations to this research. First, as noted above, the liquid TOC standard we used and the kaolin clay did not appear to add any chlorine (or chlorine dioxide) demand, which prevented our testing matrix from being as robust as originally hoped for. Second, we did not spike the samples with E. coli or other relevant microorganisms, which would have provided more information about disinfectant efficacy and the relationship between the mechanisms of chlorine demand addition and disinfectant efficacy. For example, clay could affect disinfection efficacy by making more particle surface areas available for bacteria to attach to [11]. Please note, in a recent laboratory study evaluating a 3.75 mg/L NaOCl dosage, log reductions in E. coli were high (5.0–7.3 log after 1 hour) across kaolin clay-based turbidities ranging from 10 to 300 NTU [50]. Third, we intended to use chloramines as another alternative to NaOCl and NaDCC, however preliminary laboratory testing suggested that chloramine T tablets would not release free chlorine even under the least challenging conditions (zero TOC and turbidity), possibly indicating that the tested product relies on bound chlorine (from chloramines) for disinfection. Fourth, we did not quantify TOC in our laboratory studies, which would have been particularly important for creek-bed sediments as they were collected on different days over the course of the study; additionally, increasing creek-bed sediment turbidity could have increased TOC. Lastly, we assumed that the CT-factors for NaOCl are appropriate for NaDCC.
Further research should be conducted to determine how each disinfectant responds to spikes of representative bacteria, viruses, and protozoa. Additionally, CT-factors for NaDCC should be determined in order to further compare the effectiveness of NaDCC and NaOCl. Future work is needed on developing accurate and appropriate recommendations for test waters with controlled chlorine demand to challenge all available and newly-developed chlorine-based HWT products.

Supplementary Materials

The following are available online at, Table S1: Comparison of disinfectants: p-values from the post-hoc Tukey test. Table S2: Calculated CT factors for the three disinfectants under all tested conditions. Figure S1: Disinfectant residual concentration over time at varying turbidity from clay and varying added TOC for the 4 mg/L dosage.

Author Contributions

Conceptualization, D.L.; methodology, D.L., D.S., and K.G.; validation, D.L. and K.G.; formal analysis, K.G.; investigation, D.S. and K.G.; resources, D.L.; data curation, D.S. and K.G.; writing—original draft preparation, D.L., K.G., and D.S.; writing—review and editing, D.L., G.S., K.G., and D.S.; visualization, K.G.; supervision, D.L.; project administration, D.L.; funding acquisition, D.L.


This research received no external funding. It was supported by the Tufts Summer Scholars program and the Cataldo Fellowship (both internal research support programs at Tufts University).


We would like to thank Medentech and Xinix for donations of the NaDCC and chlorine dioxide products used in this study. We also would like to thank Amylee Anyoha for her help in the laboratory.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.


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Figure 1. Testing matrix.
Figure 1. Testing matrix.
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Figure 2. Disinfectant residual concentration over time at constant added TOC (10 mg/L) and varying turbidity from creek-bottom sediments.
Figure 2. Disinfectant residual concentration over time at constant added TOC (10 mg/L) and varying turbidity from creek-bottom sediments.
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Figure 3. Disinfectant residual concentration over time at varying turbidity from clay (left) and varying added TOC (right) for the 2 mg/L dosage.
Figure 3. Disinfectant residual concentration over time at varying turbidity from clay (left) and varying added TOC (right) for the 2 mg/L dosage.
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Table 1. Characteristics of the three tested disinfectants.
Table 1. Characteristics of the three tested disinfectants.
CompoundChemical FormulaMol. Weight (g/mol)pH in SolutionBiocidal CompoundTypical Packaging
Sodium hypochloriteNaOCl74.4411–12HOClLiquid
Sodium dichloroisocyanurateNaC3N3O3Cl2219.956–7HOClPowder, tablets
Chlorine dioxideClO267.45n/aClO2 (g)Liquid, tablets
Table 2. First-order decay constants k (h−1) for sodium hypochlorite (NaOCl), sodium dichloroisocyanurate (NaDCC), and chlorine dioxide (ClO2) (based on duplicates grouped across three added total organic carbon (TOC) concentrations levels, n = 6 for each condition).
Table 2. First-order decay constants k (h−1) for sodium hypochlorite (NaOCl), sodium dichloroisocyanurate (NaDCC), and chlorine dioxide (ClO2) (based on duplicates grouped across three added total organic carbon (TOC) concentrations levels, n = 6 for each condition).
Turbidity TypeTurbidity Level [41]NaOCl k (h−1)NaDCC k (h−1)ClO2 k (h−1)
2 mg/L 4 mg/L 2 mg/L 4 mg/L 2 mg/L 4 mg/L
ClO2 decay rates are not significantly different from those of NaOCl and NaDCC (p-value 0.193–0.841). *NaDCC and NaOCl decay rates are significantly different (p < 0.001–0.012).
Table 3. Mean (standard deviation) disinfectant concentration after 24 hours (n = 6, duplicates at three added TOC levels for each turbidity and dosage tested).
Table 3. Mean (standard deviation) disinfectant concentration after 24 hours (n = 6, duplicates at three added TOC levels for each turbidity and dosage tested).
Turbidity TypeTurbidity Level [41]NaOCl (mg/L Cl2)NaDCC (mg/L Cl2)ClO2 (mg/L ClO2)
2 mg/L 4 mg/L 2 mg/L 4 mg/L 2 mg/L 4 mg/L
N/A01.7 (0.09)3.6 (0.13)1.8 (0.11)3.7 (0.18)0.29 (0.02)0.50 (0.05)
Clay101.7 (0.09)3.6 (0.09)1.9 (0.11)4.0 (0.14)0.17 (0.07)0.31 (0.13)
301.6 (0.07)3.3 (0.21)1.8 (0.11)3.9 (0.10)0.19 (0.05)0.31 (0.15)
1001.6 (0.08)3.5 (0.16)1.8 (0.11)3.8 (0.11)0.17 (0.10)0.32 (0.17)
3001.6 (0.11)3.5 (0.17)1.8 (0.08)3.7 (0.17)0.20 (0.10)0.33 (0.16)
Sediment101.0 (0.06)2.6 (0.19)1.2 (0.06)3.1 (0.16)0.16 (0.04)0.32 (0.09)
300.39 (0.17)1.2 (0.06)0.64 (0.17)2.3 (0.19)0.12 (0.01)0.29 (0.07)
1000.03 (0.01)0.12 (0.05)0.04 (0.01)0.39 (0.14)0.10 (0.03)0.21 (0.03)
3000.03 (0.01)0.06 (0.01)0.05 (0.01)0.07 (0.02)0.12 (0.04)0.19 (0.03)
Shaded cells indicate concentrations under the WHO/CDC threshold of 0.2 mg/L after 24 hours.
Table 4. CT-factors (concentration × time) from the literature for free chlorine and ClO2 against selected test organisms.
Table 4. CT-factors (concentration × time) from the literature for free chlorine and ClO2 against selected test organisms.
Test OrganismDisinfectantCT Factor (mg min/L)Inactivation*Temp. (°C)Source
Escherichia coliHOCl (NaOCl)0.04 2-log5[45]
RotavirusHOCl (oxidized HCl)1.25–3.573-log20[46]
Giardia lambliaHOCl (unclear)<9–122-log25[47]
*Required for a 2-star rating according to the World Health Organization household water treatment (WHO HWT) evaluation scheme [49].

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Gallandat, K.; Stack, D.; String, G.; Lantagne, D. Residual Maintenance Using Sodium Hypochlorite, Sodium Dichloroisocyanurate, and Chlorine Dioxide in Laboratory Waters of Varying Turbidity. Water 2019, 11, 1309.

AMA Style

Gallandat K, Stack D, String G, Lantagne D. Residual Maintenance Using Sodium Hypochlorite, Sodium Dichloroisocyanurate, and Chlorine Dioxide in Laboratory Waters of Varying Turbidity. Water. 2019; 11(6):1309.

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Gallandat, Karin, David Stack, Gabrielle String, and Daniele Lantagne. 2019. "Residual Maintenance Using Sodium Hypochlorite, Sodium Dichloroisocyanurate, and Chlorine Dioxide in Laboratory Waters of Varying Turbidity" Water 11, no. 6: 1309.

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