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Article

Comparison of Element Concentrations (Ba, Mn, Pb, Sr, Zn) in the Bones and Teeth of Wild Ruminants from the West Carpathians and the Tian-Shan Mountains as Indicators of Air Pollution

by
Zuzana Ballová
1,*,
Marián Janiga
1 and
Richard Hančinský
1,2
1
Institute of High Mountain Biology, University of Žilina, SK-05956 Tatranská Javorina 7, Slovakia
2
Department of Biotechnology, University of St. Cyril and Methodius, Nám. J. Herdu 2, 917 01 Trnava, Slovakia
*
Author to whom correspondence should be addressed.
Atmosphere 2019, 10(2), 64; https://doi.org/10.3390/atmos10020064
Submission received: 5 November 2018 / Revised: 16 January 2019 / Accepted: 24 January 2019 / Published: 2 February 2019
(This article belongs to the Special Issue Atmospheric Nutrients: Sources, Processes and Impact on Terrestrial and Marine Ecosystems)
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Abstract

:
Through analyzing the concentrations of selected heavy metals (Ba, Mn, Pb, Sr, Zn) in the bones and teeth of wild living and ecologically equivalent ruminants from the Tian-Shan (Capra sibirica and Ovis ammon polii) and the West Carpathians (Rupicapra rupicapra tatrica) we compared the environmental pollution levels of these two mountain ranges. The samples were analyzed by X-ray fluorescence. Significantly higher contents of Zn and Mn as well as a higher frequency of measurable occurrences of Mn, Ba, and Pb in samples from the West Carpathians confirmed the results of our previous study, that the West Carpathians are relatively more polluted by heavy metals than the Tian-Shan Mountains. The most probably contamination sources are mining and smelting as well as traffic emissions, which can reach remote mountain ranges through long distance atmospheric transport.

1. Introduction

Anthropogenic emissions from the extended territory of the Soviet Union and China considerably influenced the concentration of heavy metals in the Northern Hemisphere [1,2,3,4]. Quantitative planetary boundaries for aerosol loading and chemical pollution have not yet been established and there is insufficient knowledge to suggest them [5].
The Tian-Shan is one of the most polluted mountain ranges in central Asia by anthropogenic sources of heavy metals [2,3,6,7,8]. According to a study on ice core records from the Inilchek glacier in central Tian-Shan, pollutant sources originated primarily from Kazakhstan, Uzbekistan and Kyrgyzstan [2]. When considering the data presented in the State Statistic Committee of the Kyrgyzstan Republic’s report in 2015, the volume of pollution released into the atmosphere from stationary pollution sources increased by 44 percent compared to 2011. Such a sharp increase in pollution is related to the increased use of coal by large scale heating plants due to low water periods. The emission of sulphur dioxide as one of the main air pollutants increased by 3.8 percent between 2014 and 2015 [9].
The environment as a whole is being polluted, including wild animals living within it. Because animals living in the wild depend exclusively on the natural resources and the condition of soils present in their habitats, the effects of such pollution can be analyzed without provable influence from direct anthropogenic factors. Environmental pollution caused by harmful substances can affect the animals inhabiting a given territory via the food or water they ingest and the air they breathe [10]. Toxic metals can accumulate in soil and plants and may subsequently enter the animal food chain and threaten their health. Heavy metals have a strong influence on nutritional values in plants (effects on protein, amino acids, carbohydrates, fats, and vitamins); therefore, plants grown in metal-contaminated soil are nutrient deficient and consumption of such vegetation may lead to nutritional deficiency in the consuming population [11]. Knowing that alpine ecosystems are often the main accumulation sites of trace metals from the atmosphere and that the concentrations of these pollutants increase with altitude, the main sources of trace metals for the plants that ruminants eat are atmospheric pollutants [8,12].
In Tian-Shan alpine ecosystems, there live two protected wild species of ruminants; the Asiatic ibex (Capra sibirica, Pallas 1776) and the Marco Polo sheep (Ovis ammon polii, Blyth 1840).
C. sibirica is typically found in the mountains of central Asia. In the central Tian-Shan (China), the iron mining industry developed rapidly in recent years and caused damage to the original habitat of ibex in the affected areas. Mine production operations, especially large transport vehicles, made ibex more sensitive to human disturbance [13]. Ibex numbers in the Tian-Shan of Kazakhstan may have declined in some areas [14].
Marco Polo sheep numbers have decreased greatly in the past because of uncontrolled hunting. Due to over-hunting and subsistence poaching, as well as competition with livestock and habitat loss, O. ammon has been categorized as an endangered species on several lists [15]. Uncontrolled killing of the Marco Polo sheep by firearms appears to be common; local militia and customs officials have killed dozens with gun-machines. In the Kara-Tau Mountains, the population could have been as low as 100 animals [16]. With regard to that information, the populations of both ruminants are relatively seriously endangered. Their populations may also be affected by atmospheric pollution, mainly by toxic heavy metals that accumulate in their bodies.
We have previously confirmed that the Carpathians are still one of the most polluted mountain ranges, and indicated that the Tatra mountains (West Carpathians) are more polluted by Pb than Tian-Shan [17,18]. In our previous study, we compared atmospheric pollution between Tian-Shan and European high mountain ranges (the Tatra, Vitosha and Rila mountains) via short living rodent species [17]. In terms of long-term monitoring, it is better to use tissues from longer-living herbivorous animals, which indicate primarily their accumulation of heavy metals from plants. We therefore investigated the heavy metal content in bones of the Tatra chamois (Rupicapra rupicapra tatrica, Blahout 1972), which is an equivalent ruminant species with similar habitat and food requirements to C. sibirica and O. ammon from the Tian-Shan mountains. Chamois (Rupicapra genera) are indigenous to several mountain ranges in Europe and also in Asia, and have been successfully introduced into new countries with mountain habitats such as New Zealand. The Tatra chamois is an endemic and protected species, inhabiting alpine and subalpine areas of the Tatra mountains.
Among heavy metals, essential elements, such as Mn and Zn in this study, ensure the normal development and function of the organism, whereas Ba, Pb and Sr are toxic elements which often cause acute and chronic environmental contamination. All of these metals can be transported through atmospheric flows. The target organs for Mn accumulation are bones, and Mn toxicity has been associated with dopaminergic dysfunction [19]. Mn exposure impacts neurotransmitter levels in the brain. Chronically high Mn levels in the brain are neurotoxic and can result in a progressive, irreversible neurological disorder known as manganism [20]. There are three common methods of absorption into the body (inhalation, through the skin, and by ingestion) [21]. The most probable route of entry for Zn into the animal body in alpine environments is by ingestion. Effects associated with long-term, excessive Zn intake include nausea, epigastric pain, diarrhea, elevated risk of prostate cancer, copper deficiency and sequelae, altered lymphocyte function, lethargy and focal neuronal deficits [22]. Environmental exposure to Ba causes cardiovascular and kidney diseases, as well as metabolic, neurological, and mental disorders [23]. Pb is a general accumulative metabolic poison affecting the hematopoietic, cardiovascular, nervous, renal, and reproductive systems [24]. Sr affects food intake, body weight gain [25] and bone biochemistry [26]. Ba, Pb, and Sr are mainly deposited in the skeleton, which is the major deposition site for many metals, where they are incorporated into bone minerals [27].
The main objective of our research was to compare contents of selected heavy metals (Ba, Mn, Pb, Sr, Zn) between two different mountain ranges. Because calcified tissues are good bioindicators of long-term metal accumulation [27,28], we analyzed concentrations of selected heavy metals in the bone and tooth tissues of wild living and ecologically equivalent ruminants from Tian-Shan (C. sibirica and O. ammon) and the Tatra mountains (R. r. tatrica).

2. Experiments

2.1. Sample Collection and Analyses

Knowing that heavy metal concentrations increase with altitude much more than with soil conditions or other ecological factors [8,29], to determine element concentrations we used bone and tooth tissues from wild ruminants of the Bovidae family living in alpine environments. Different bone and tooth tissues (axillar and appendicular skeleton, teeth) were used based on the possibility of finding samples in the field. According to some studies, types of bone and tooth tissues have less effect on the trace element content than the environmental distribution of samples with distinctive levels of elements [30,31]. Our samples were collected from two geographically isolated mountain ranges with distinctive possible pollution sources. In the Tian-Shan (N41.81961667°, E77.58944444°; Republic of Kyrgyzstan), we used samples from the Asiatic ibex (Capra sibirica) (Figure 1a) and the Marco Polo sheep (Ovis ammon polii) (Figure 1b). The samples were collected during spring and autumn in 2013. The altitude of the study sites in the Tian-Shan (Figure 2a) ranged from 2616 to 3545 m a.s.l. In the West Carpathians (Slovakia), samples from Rupicapra rupicapra tatrica were collected in alpine areas of the Tatra mountains (N49.16472222°, E20.13416667°) (Figure 2b) over a sixteen-year period (1993–2009 m a.s.l.).

2.2. X-ray Fluorescence Spectrometry

The samples were analyzed by X-ray fluorescence, using the hand-held XRF Spectrometer DELTA CLASSIC (Innov-X Systems, Inc., Woburn, MA, USA.) [32]. The handheld X-ray fluorescence was already used for scanning the hard tissues including horns, antlers, teeth and bones and it was demonstrated that a handheld XRF is an effective and accurate tool for analytical investigation [33]. We used the CONOSTAN calibration standard [34]. Samples of bones and teeth were mechanically cleaned and rinsed with distilled water, to prevent contamination of the sample with surface extrinsic elements. Subsequently, the upper layers of bones were carefully ground because of accumulated impurities from the environment, in order to obtain pure bone element contents. A direct method of analysis, without the usage of cuvettes nor homogenization of samples was used. We used multiple-beam measurement, in which every measurement consisted of 3 beams for 30 s, repeated three times, and then averaged. The results were given in ppm (part per million) units. We used 38 samples (tooth and bone tissues) from the Tian-Shan and 30 from the West Carpathians.
Our instrument was certified and verified by multiple measurements. We measured two soil matrix standards (No. 2710a and 2711a) [35,36] and one beef liver standard (NCS ZC 71001) [37]. There were minimal standard deviations between certified values and our measured values. We also repeated measurements on soil matrix standards, to demonstrate repeatability. The standard deviation (SD) was stable and minimal for all repeated measurements.
Using the multiple testing of our bone and tooth samples we found minimum values of the concentrations of distinct elements that our instrument was able to measure, in constant conditions. Limits of detection for each measured metal were determined from samples, in which the content of this metal was at the limit of detection. The minimum content of a particular element represented the current detection limit of the spectrometer for the measured material [38]. The minimum values are presented in Table 1 for the Tian-Shan and in the Table 2 for the West Carpathians.
Certified limits of detection of selected elements measured in our samples for the handheld XRF Spectrometer DELTA CLASSIC using the soil matrix are: Mn: 10–30 ppm, Zn: 10–15 ppm, Ba: 40–60 ppm, Pb: 5–10 ppm, and Sr: 3–5 ppm [39].

2.3. Statistics

For statistical analysis, we chose elements measured in our samples with the highest accuracy that were found in both mountain ranges: Ba, Mn, Pb, Sr, and Zn. Data were standardized considering the possible eccentricity of some measurements. The average values of the elements were calculated. The Chi-squared test was applied to data with an abundance of positive and negative measurements of elements in the samples from different mountain ranges (p < 0.05). In cases where sample size was recorded as 20 to < 40, but an expected value was < 5, we used the Fisher’s Exact test (case of Zn). In the case of two zero values in the cells with total observation values, frequencies of element occurrence in different mountain ranges could not be evaluated (case of Sr). Because the distribution of observed levels of trace elements was normal according to the Shapiro-Wilk test, the parametric Student’s t-test was used to compare element concentrations between different mountain ranges. Statistical analyses were performed with Statistica 12 software for Windows (Stat Soft CR, Prague, Czech Republic).

3. Results

We determined the presence of the following elements in our samples from both mountain ranges: P, S, Cl, K, Ca, Ti, Cr, Mn, Fe, Ni, Cu, Zn, Rb, Sr, Zr, Mo, Ag, Sn, I, Ba, Hg, and Pb. Other elements, which were measured but were less than detection limit in all our samples, included Co, As, Se, Cd and Sb. Sr was found in all of our samples from the Tian-Shan as well as from the West Carpathians. Zn was measured in all samples from the West Carpathians. Selected heavy metals (Ba, Mn, Pb, Sr, Zn) were subsequently used for comparison of these two regions.
We found significant differences in the frequency of occurrences of Mn, Ba, Pb and Zn between the two mountain ranges, whereas Sr was measured in all samples from both mountain ranges (Table 3).
For each element, the means were calculated only from samples in which the amount of an element was above the detection limit of the spectrometer DELTA CLASSIC (Table 1 and Table 2).
Significant differences in element concentrations between mountain ranges were found only for Zn and Mn (Table 4).

4. Discussion

It was found that Mn is poorly absorbed by ruminants [40]. Thus, the higher content of this element in animal bones is most likely the result of a long-lasting increase in exposure. The ‘‘normal’’ range of mammalian tissue Mn concentrations is 0.3–2.9 ppm [41]. However, bone, brain, liver, pancreas, and kidney tissue have high Mn concentrations [41]. This element was found in more samples and with higher content in the West Carpathians than in the Tian-Shan mountains. The major pool of Mn in soils originates from crustal rock, with other sources including direct atmospheric deposition, wash-off from plants and other surfaces, leaching from plant tissues, and the shedding or excretion from plant and animal matter [42]. The major anthropogenic source of environmental Mn include emission from Mn is mining. Another emerging source of Mn pollution is the gasoline additive methylcyclopentadienyl manganese tricarbonyl (MMT), an organic derivative of Mn that was introduced to automobile fuel formulas as an octane-boosting and antiknock agent [42]. According to a study on sediments in Smreczynski Staw Lake in the Tatra mountains, Mn concentrations have increased since the beginning of the 20th century, but most significantly since the end of the 20th century. This could be the result of transportation from remote sources and acid rain [43]. Contrary to the West Carpathians, the Inilchek glacier, located in central Tian-Shan, was recognized to contain a conservative natural crustal element, from metal dust containing Mn [2]. The majority of our samples originated from the Ysyk-Köl region (oblast), where the Inilchek glacier is located.
Ba and Pb were elements found in higher concentrations in the West Carpathians than in the Tian-Shan mountains (Table 1 and Table 2). Pb was found in only one of the samples from the Tian-Shan mountains, but in many samples (n = 17) from the West Carpathians (Table 3). This may be caused by traffic emissions. The highest emission levels in Central Asian countries are associated with big cities, which are further away from mountain ranges than they are in Europe [44]. Pb levels in Tatra lake sediments, increased in the samples from the 20th century, and are likely deposited from air pollution resulting from the development of road transport [43]. Ba is present in diesel and unleaded gasoline, so the element was recognized as a valuable tracer for vehicle emissions, in the place of Pb. Ba and Pb are both connected with vehicle emissions, so the presence of these elements is most likely a result of the differences in emission transport between the two mountain ranges [45]. In the Tian-Shan Inilchek glacier, Pb levels showed a decline during the 1980s in conjunction with the Soviet economic decline. Due to the rapid industrial and agricultural growth of western China, Pb increased during the 1990s, reflecting a transition from primarily central Asian sources to emission sources from western China (e.g., Xinjiang Province) [2]. It was found that coal burning emissions and Pb in vehicle gasoline were major sources of heavy Pb pollution in the Tian-Shan mountains region [3].
We found that R. r. tatrica, as the only West Carpathian ruminant species living in alpine habitats, had significantly higher levels of Zn than ruminants from the Tian-Shan mountains. Although anthropogenic emissions from the extended territory of the Soviet Union and China considerably influenced concentrations of heavy metals in the Northern Hemisphere, according to the results of several studies, Zn emissions have declining trends. These can be attributed to the economic downturn in industry, changes in technology to an increasing metal recovery from ores, the replacement of coal and oil by gas, and air pollution control [1,46,47]. Potential sources of heavy metals in the Tatra mountains may be northern Moravia in the Czech Republic and the Małopolska district in southern Poland with Zn-Pb mines and smelters [48,49,50,51]. In addition, our previous studies show high levels of Zn were found in the bones and teeth of Tatra marmots, with this element being detected in all samples [38,52]. In a study of snow voles from the Tatra mountains, Zn was also found in all samples, and the mean amount of Zn in bone tissues was 72.49 ppm ± 24.76 [53].
One-time uptake of Zn in higher concentrations from food sources is almost immediately excreted from the organism by feces. It is only after a long period of higher uptake that the ruminant will adapt to higher Zn levels [54]. It follows then, that there is a higher concentration of Zn in ruminant food sources in the West Carpathians than in the Tian-Shan mountains. This is, of course, applicable only for non-toxic dosages [54]. Furthermore, it was found that Zn was the most responsive to excessive supply and is deposited more commonly in bones compared to other body tissues [55]. Therefore, bones of ruminants can be a very good indicator of Zn availability in the area.
Main sources of environmental contamination of Zn and Pb, along with other heavy metals, include the coal and mining industries [56,57,58,59,60]. We expect that the Zn and Pb pollutants in our samples from the West Carpathians were transported by atmospheric flows from Zn-Pb smelters due to prevailing north-western winds in the Tatra mountains. However, Pb and Zn have not been mined in the neighboring Czech Republic recently, and mining and smelting of these heavy metals was also reduced in Poland [51]. Pb and Zn ore is still mined in southeastern Poland in the Silesia-Cracow region at two underground mines (the Olkuz-Pomorzany Mine and the Trzebionka Mine) [51], which are close to the Tatra mountains. In soils sampled at historical metal mining sites in western Małopolska, Pb content ranged from 72.8 to 16931 and Zn content ranged from 322 to 41860 ppm. These levels are indicative of heavy contamination of the surrounding environment by these metals [50].
Zn levels in the bones of Tatra chamois were similarly high compared to those found in bones of small rodents from Zn polluted areas in Slovakia [56,61]. Zn and Pb pollution in Slovakia may also be attributed to prevailing north-western winds from the highly polluted Upper Nitra region, which is home to various anthropogenic sources of pollution (chemical plant, coal power station, coal mines, stone and limestone pits, aluminum production, factories, and intensive agricultural production), as confirmed by several studies [28,56,61].
Zn accumulation due to traffic emissions should not be ignored. However, studies on heavy metals emitted by vehicles in Florence show that Ba, Mn, Pb, and Zn, along with Cu and Fe are all pollutants resulting from emissions [60]. Zn contaminants from mining and smelting, along with pollutants emitted by diesel-engine vehicles, could be deposited on mountain ridges through wet and dry atmospheric deposition.
Although Sr concentration results were non-significant, slightly higher mean values were measured in samples from the Tian-Shan mountains. Sr occurs frequently as an isotope in rocks. Due to weathering and hydrologic cycles, Sr from these rocks penetrates into soils, plants, and subsequently into animal skeletons [61]. In bones, Sr is highly related to P content [38]. It was found that Sr in bones decreases as dietary phosphorus increases [62]. When we consider that differing Sr values mainly occurred between the two species from the Tian-Shan mountains, not between species from different mountain ranges, we can potentially attribute them to the distinctive dietary preferences of each species [63], as different plants, mosses or lichens and different plant tissues contain specific concentrations of elements [8].
The concentration of (Ba, Mn, Pb, Sr, and Zn) in the West Carpathians may, in addition to mentioned sources, be associated to some extent with emissions from the paper and pulp factory located in Ružomberok (a town situated in north-western Slovakia). Several studies were conducted dealing with these heavy metal pollutants from the Ružomberok industrial zone [64,65,66]. This industrial zone is located near the Tatra mountain range and through prevailing north-western winds the emissions could easily be transported to alpine areas of the West Carpathians, as was also partially confirmed by a study on elements in the needles of Abies alba from the West Carpathians [67].
The content of elements in bone tissues may also be impacted by the exposure of the bones to the elements in the terrain. Bones from the terrain were already used for element analysis [68], but some studies show that element concentrations in older bones can be influenced by the presence of microorganisms, which, while helping to decompose the bone, impact element concentrations [69]. The element ratios in different, old bone samples may also be affected by biological degradation and environmental leaching [70]. In our study we used bone samples without evidence of visible marks of weathering or decomposition to mitigate this effect, and the samples were carefully cleaned and ground.
Tissue type (bone or tooth) may also potentially impact results. We used two different tissues: Bones and teeth. The analysis of ruminant permanent teeth is a useful indicator for assessing life-long intoxication by environmental pollution [71]. However, the concentrations of Ba, Mn and Zn in teeth of roe deer showed positive linear relationships with individual age. No such trends were recorded for trace element content in bones [71].

5. Conclusions

We found significant differences in the accumulation of Mn and Zn between bone samples from the Tian-Shan mountains and from the West Carpathians. The higher content of Zn and Mn pollutants in samples from the West Carpathians is likely attributable to mining and smelting, as well as road transport emissions in adjacent regions to the Tatra mountains. When analyzing the frequency of occurrence of selected heavy metals (Mn, Ba, Pb, Sr, Zn), we found significantly higher frequencies of measurable Mn, Ba, and Pb in samples from the West Carpathians than from the Tian-Shan mountains. Significant amounts of heavy metals (Mn, Ba, Pb, Sr, Zn) are discharged in the atmosphere from anthropogenic sources in the north-west parts of Europe and through the prevailing north-western winds transported to alpine areas of the West Carpathians, where atmospheric contaminants accumulate. Metal pollutants deposited to the surface of the Earth from the air gradually penetrate into soils, plants, and subsequently into animal skeletons. We found that the Tian-Shan mountains are less polluted by some heavy metals than the West Carpathians. This could be a result of the longer history of industrialization in Western Europe as well as relatively shorter distances between pollutant sources in Slovak, Poland and the Czech Republic to sample sites in the Tatra mountains. Trace metals in bones and teeth of wild ruminants have been shown to be qualitative indicators of heavy metal contamination from atmospheric deposition in high mountain ecosystems.

Author Contributions

Z.B. gave comments and ideas on the research and was responsible for statistical and ecological analyses, interpretation of the results, and writing the manuscript. M.J. gave basic ideas on the research and was responsible for field data collection, statistical and ecological analyses, interpretation of the results, and editing the manuscript. R.H. gave comments and ideas on the research and was responsible for field data collection, laboratory and ecological analyses, interpretation of the results, and writing parts of the manuscript.

Funding

This research was funded by European Structural Funds (ITMS, Project number: 26210120006).

Acknowledgments

For English proofreading and improving, we are indebted to native English-speaking editor Amanda Clarahan.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Atmosphere 10 00064 g001 550
Figure 1. Bones contain useful information on some metal contamination in the mountains. Hundreds of (a) Asiatic ibex and (b) Marco Polo sheep skulls may be found in the valleys of Kyrgyzstan. The animals are hunted by local people for meat and heavy heads are usually left in the field. Photo: M. Janiga, 2013.
Figure 1. Bones contain useful information on some metal contamination in the mountains. Hundreds of (a) Asiatic ibex and (b) Marco Polo sheep skulls may be found in the valleys of Kyrgyzstan. The animals are hunted by local people for meat and heavy heads are usually left in the field. Photo: M. Janiga, 2013.
Atmosphere 10 00064 g001
Atmosphere 10 00064 g002 550
Figure 2. Maps of sample sites in (a) the Tian-Shan mountains (Kyrgyzstan) and (b) the Tatra mountains (Slovakia). The sample sites are displayed as circles.
Figure 2. Maps of sample sites in (a) the Tian-Shan mountains (Kyrgyzstan) and (b) the Tatra mountains (Slovakia). The sample sites are displayed as circles.
Atmosphere 10 00064 g002
Table
Table 1. Concentrations of the examined elements in the hard tissues (bones and teeth together) of ruminants from the Tian-Shan mountains.
Table 1. Concentrations of the examined elements in the hard tissues (bones and teeth together) of ruminants from the Tian-Shan mountains.
Element (ppm)NMeanMedianMinimumMaximumSD
Ba13101.0896.0054.00211.0041.22
Pb18.008.008.008.00-
Sr38200.61187.50129.00593.0077.75
Zn3257.5337.5014.00253.0054.62
Mn2082.4562.5017.00377.0083.12
Table
Table 2. Concentrations of the examined elements in the hard tissues (bones and teeth together) of ruminant species from the West Carpathians.
Table 2. Concentrations of the examined elements in the hard tissues (bones and teeth together) of ruminant species from the West Carpathians.
Element (ppm)NMeanMedianMinimumMaximumSD
Ba1999.7985.0052.00199.0040.27
Pb1715.129.008.0040.0010.97
Sr30156.47140.0055.00711.00112.99
Zn30175.73143.0053.00470.0093.95
Mn29167.34125.0020.00764.00163.20
Table
Table 3. Contingency table with the frequencies of selected five elements and associations of its occurrences between positive and negative bone samples from the Tian-Shan mountains (TS) and the Tatra mountains (WC).
Table 3. Contingency table with the frequencies of selected five elements and associations of its occurrences between positive and negative bone samples from the Tian-Shan mountains (TS) and the Tatra mountains (WC).
Sample TypeMn (n = 68)Ba (n = 68)Pb (n = 68)Sr (n = 68)Zn (n = 68)
WC positive(29) 42.6%(19) 27.9%(17) 25.0%(30) 44.1%(30) 44.1%
TS positive(20) 29.4%(13) 19.1%(1) 1.5%(38) 55.9%(32) 47.1%
WC negative(1) 1.5%(11) 16.2%(13) 19.1%(0) 0.0%(0) 0.000%
TS negative(18) 26.5%(25) 36.8%(37) 54.4%(0) 0.0%(6) 8.8%
Chi-square WC/TS (df = 1)16.15,
p = 0.0001		5.71,
p = 0.0169		25.15,
p = 0.000		-p = 0.0252 1
1 Fisher’s Exact test.
Table
Table 4. Comparison of the concentrations of selected five element measurements between bone samples from the Tian-Shan mountains and the Tatra mountains by the Student’s t-test.
Table 4. Comparison of the concentrations of selected five element measurements between bone samples from the Tian-Shan mountains and the Tatra mountains by the Student’s t-test.
Elementt-ValuedfpN(TS)N(WC)F-Ratiop
Ba0.09300.930513191.050.9019
Pb−0.63160.53721170.001.0000
Sr1.91660.061138302.110.0326 *
Zn−6.10600.0000 ***32302.960.0038 ***
Mn−2.14470.0377 **20293.860.0034 ***
*** p < 0.001; ** p < 0.01; * p < 0.05.

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Ballová, Z.; Janiga, M.; Hančinský, R. Comparison of Element Concentrations (Ba, Mn, Pb, Sr, Zn) in the Bones and Teeth of Wild Ruminants from the West Carpathians and the Tian-Shan Mountains as Indicators of Air Pollution. Atmosphere 2019, 10, 64. https://doi.org/10.3390/atmos10020064

AMA Style

Ballová Z, Janiga M, Hančinský R. Comparison of Element Concentrations (Ba, Mn, Pb, Sr, Zn) in the Bones and Teeth of Wild Ruminants from the West Carpathians and the Tian-Shan Mountains as Indicators of Air Pollution. Atmosphere. 2019; 10(2):64. https://doi.org/10.3390/atmos10020064

Chicago/Turabian Style

Ballová, Zuzana, Marián Janiga, and Richard Hančinský. 2019. "Comparison of Element Concentrations (Ba, Mn, Pb, Sr, Zn) in the Bones and Teeth of Wild Ruminants from the West Carpathians and the Tian-Shan Mountains as Indicators of Air Pollution" Atmosphere 10, no. 2: 64. https://doi.org/10.3390/atmos10020064

APA Style

Ballová, Z., Janiga, M., & Hančinský, R. (2019). Comparison of Element Concentrations (Ba, Mn, Pb, Sr, Zn) in the Bones and Teeth of Wild Ruminants from the West Carpathians and the Tian-Shan Mountains as Indicators of Air Pollution. Atmosphere, 10(2), 64. https://doi.org/10.3390/atmos10020064

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