Complement Factor C3 Methylation and mRNA Expression Is Associated to BMI and Insulin Resistance in Obesity
Abstract
1. Introduction
2. Materials and Methods
2.1. RNA Isolation and Gene Expression
2.2. Methylation Analysis DNA
2.3. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Overweight (n = 23) | Class 1/2 (n = 20) | Class 3 (n = 17) | |
---|---|---|---|
Age (years) | 55.70 ± 11.71 a | 56.70 ± 15.24 a | 41.53 ± 9.78 b |
Gender (men/women) | 10/13 | 7/13 | 6/11 |
BMI (kg/m2) | 27.41 ± 1.29 a | 33.23 ± 2.76 b | 49.78 ± 6.49 c |
Waist (cm) | 93.96 ± 5.48 a | 106.75 ± 9.23 b | 133.69 ± 17.77 c |
Glucose (mmol/L) | 6.12 ± 1.20 | 5.99 ± 1.47 | 5.79 ± 1.15 |
HOMA-IR | 2.318 ± 0.98 a | 3.124 ± 1.59 a | 5.30 ± 4.91 b |
Tg (mmol/L) | 1.42 ± 0.60 | 1.47 ± 0.54 | 1.37 ± 0.50 |
Cholesterol (mmol/L) | 5.58 ± 1.00 a | 5.48 ± 1.28 a | 4.67 ± 0.94 b |
HDL-cho (mmol/L) | 1.37 ± 0.34 a,b | 1.49 ± 0.32 a | 1.26 ± 0.32 b |
SBP (mmHg) | 131.43 ± 22.37 | 135.65 ± 24.83 | 136.07 ± 19.39 |
DBP (mmHg) | 79.52 ± 12.62 | 79.75 ± 12.78 | 83.79 ± 10.03 |
ApoA1 (mmol/L) | 1.73 ± 0.20 | 1.81 ± 0.27 | 1.58 ± 0.15 |
ApB (mmol/L) | 1.10 ± 0.27 | 1.01 ± 0.21 | 0.89 ± 0.28 |
GOT (µkat/L) | 0.25 ± 0.12 a | 0.34 ± 0.18 a,b | 0.39 ± 0.16 b |
GPT (µkat/L) | 0.59 ± 0.24 | 0.75 ± 0.37 | 0.75 ± 0.32 |
GGT (µkat/L) | 0.63 ± 0.50 | 0.61 ± 0.29 | 0.55 ± 0.38 |
Leptin (ng/mL) | 14.31 ± 7.13 a | 21.83 ± 11.12 a | 68.21 ± 30.09 b |
Adiponectin (ng/mL) | 9.21 ± 3.92 | 10.49 ± 4.64 | 6.90 ± 3.83 |
C3 mRNA | C3 Methylation | ASP | |
---|---|---|---|
Age | 0.076 | 0.226 | 0.035 |
BMI | 0.177 | −0.411 ** | −0.04 |
Waist | 0.192 | −0.26 | 0.062 |
Insulin | 0.364 ** | −0.079 | 0.157 |
Glucose | 0.324 * | 0.09 | −0.102 |
HOMA-IR | 0.417 ** | −0.079 | 0.124 |
HDL-cho | 0.073 | −0.176 | −0.370 ** |
Leptin | 0.289 | −0.528 ** | −0.077 |
Adiponectin | −0.316 * | 0.005 | −0.071 |
A | BMI (R = 0.69, R2 = 0.48) | ||
β | p | CI (95%) | |
Age | −0.28 | 0.00 | −0.43–(−0.13) |
Gender | 0.48 | 0.81 | −3.76–4.73 |
HOMA-IR | 1.12 | 0.00 | 0.38–1.87 |
C3 mRNA | 0.34 | 0.65 | −1.17–1.86 |
C3 methylation | −0.79 | 0.00 | −1.35–(−0.23) |
B | HOMA-IR (R = 0.59, R2 = 0.35) | ||
β | p | CI (95%) | |
Age | −0.00 | 0.96 | −0.06–0.05 |
Gender | −0.71 | 0.32 | −2.14–0.72 |
BMI | 0.13 | 0.00 | 0.04–0.21 |
C3 mRNA | 0.74 | 0.00 | 0.26–1.22 |
C3 methylation | 0.07 | 0.44 | −0.12–0.28 |
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Castellano-Castillo, D.; Moreno-Indias, I.; Fernandez-Garcia, J.C.; Clemente-Postigo, M.; Castro-Cabezas, M.; Tinahones, F.J.; Queipo-Ortuño, M.I.; Cardona, F. Complement Factor C3 Methylation and mRNA Expression Is Associated to BMI and Insulin Resistance in Obesity. Genes 2018, 9, 410. https://doi.org/10.3390/genes9080410
Castellano-Castillo D, Moreno-Indias I, Fernandez-Garcia JC, Clemente-Postigo M, Castro-Cabezas M, Tinahones FJ, Queipo-Ortuño MI, Cardona F. Complement Factor C3 Methylation and mRNA Expression Is Associated to BMI and Insulin Resistance in Obesity. Genes. 2018; 9(8):410. https://doi.org/10.3390/genes9080410
Chicago/Turabian StyleCastellano-Castillo, Daniel, Isabel Moreno-Indias, Jose Carlos Fernandez-Garcia, Mercedes Clemente-Postigo, Manuel Castro-Cabezas, Francisco José Tinahones, María Isabel Queipo-Ortuño, and Fernando Cardona. 2018. "Complement Factor C3 Methylation and mRNA Expression Is Associated to BMI and Insulin Resistance in Obesity" Genes 9, no. 8: 410. https://doi.org/10.3390/genes9080410
APA StyleCastellano-Castillo, D., Moreno-Indias, I., Fernandez-Garcia, J. C., Clemente-Postigo, M., Castro-Cabezas, M., Tinahones, F. J., Queipo-Ortuño, M. I., & Cardona, F. (2018). Complement Factor C3 Methylation and mRNA Expression Is Associated to BMI and Insulin Resistance in Obesity. Genes, 9(8), 410. https://doi.org/10.3390/genes9080410