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Article

Transcriptome-Wide Identification of an Aurone Glycosyltransferase with Glycosidase Activity from Ornithogalum saundersiae

Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College (State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products), Beijing 100050, China
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2018, 9(7), 327; https://doi.org/10.3390/genes9070327
Received: 29 May 2018 / Revised: 21 June 2018 / Accepted: 22 June 2018 / Published: 28 June 2018
(This article belongs to the Special Issue Plant Metabolic Engineering of High Value Bioactive Products)
Aurone glycosides display a variety of biological activities. However, reports about glycosyltransferases (GTs) responsible for aurones glycosylation are limited. Here, the transcriptome-wide discovery and identification of an aurone glycosyltransferase with glycosidase activity is reported. Specifically, a complementary DNA (cDNA), designated as OsUGT1, was isolated from the plant Ornithogalum saundersiae based on transcriptome mining. Conserved domain (CD)-search speculated OsUGT1 as a flavonoid GT. Phylogenetically, OsUGT1 is clustered as the same phylogenetic group with a putative 5,6-dihydroxyindoline-2-carboxylic acid (cyclo-DOPA) 5-O-glucosyltransferase, suggesting OsUGT1 may be an aurone glycosyltransferase. The purified OsUGT1 was therefore used as a biocatalyst to incubate with the representative aurone sulfuretin. In vitro enzymatic analyses showed that OsUGT1 was able to catalyze sulfuretin to form corresponding monoglycosides, suggesting OsUGT1 was indeed an aurone glycosyltransferase. OsUGT1 was observed to be a flavonoid GT, specific for flavonoid substrates. Moreover, OsUGT1 was demonstrated to display transglucosylation activity, transferring glucosyl group to sulfuretin via o-Nitrophenyl-β-d-glucopyranoside (oNP-β-Glc)-dependent fashion. In addition, OsUGT1-catalyzed hydrolysis was observed. This multifunctionality of OcUGT1 will broaden the application of OcUGT1 in glycosylation of aurones and other flavonoids. View Full-Text
Keywords: aurones; sulfuretin; glycosyltransferase; Ornithogalum saundersiae aurones; sulfuretin; glycosyltransferase; Ornithogalum saundersiae
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MDPI and ACS Style

Yuan, S.; Liu, M.; Yang, Y.; He, J.-M.; Wang, Y.-N.; Kong, J.-Q. Transcriptome-Wide Identification of an Aurone Glycosyltransferase with Glycosidase Activity from Ornithogalum saundersiae. Genes 2018, 9, 327. https://doi.org/10.3390/genes9070327

AMA Style

Yuan S, Liu M, Yang Y, He J-M, Wang Y-N, Kong J-Q. Transcriptome-Wide Identification of an Aurone Glycosyltransferase with Glycosidase Activity from Ornithogalum saundersiae. Genes. 2018; 9(7):327. https://doi.org/10.3390/genes9070327

Chicago/Turabian Style

Yuan, Shuai, Ming Liu, Yan Yang, Jiu-Ming He, Ya-Nan Wang, and Jian-Qiang Kong. 2018. "Transcriptome-Wide Identification of an Aurone Glycosyltransferase with Glycosidase Activity from Ornithogalum saundersiae" Genes 9, no. 7: 327. https://doi.org/10.3390/genes9070327

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