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Genes 2018, 9(2), 99; https://doi.org/10.3390/genes9020099

Characterization of the Symbiotic Nitrogen-Fixing Common Bean Low Phytic Acid (lpa1) Mutant Response to Water Stress

1
Center for Genomic Sciences, National Autonomous University of Mexico, Av, Universidad 1001, Cuernavaca 62210, Mor., Mexico
2
Institute of Agricultural Biology and Biotechnology, National Research Council, IBBA-CNR, Via Edoardo Bassini 15, 20133 Milano, Italy
*
Author to whom correspondence should be addressed.
Received: 13 December 2017 / Revised: 5 February 2018 / Accepted: 12 February 2018 / Published: 15 February 2018
(This article belongs to the Special Issue Genetics and Genomics of the Rhizobium-Legume Symbiosis)
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Abstract

The common bean (Phaseolus vulgaris L.) low phytic acid (lpa1) biofortified genotype produces seeds with improved nutritional characteristics and does not display negative pleiotropic effects. Here we demonstrated that lpa1 plants establish an efficient nitrogen-fixing symbiosis with Rhizobium etli CE3. The lpa1 nodules showed a higher expression of nodule-function related genes than the nodules of the parental wild type genotype (BAT 93). We analyzed the response to water stress of lpa1 vs. BAT 93 plants grown under fertilized or under symbiotic N2-fixation conditions. Water stress was induced by water withholding (up to 14% soil moisture) to fertilized or R. etli nodulated plants previously grown with normal irrigation. The fertilized lpa1 plants showed milder water stress symptoms during the water deployment period and after the rehydration recovery period when lpa1 plants showed less biomass reduction. The symbiotic water-stressed lpa1 plants showed decreased nitrogenase activity that coincides with decreased sucrose synthase gene expression in nodules; lower turgor weight to dry weight (DW) ratio, which has been associated with higher drought resistance index; downregulation of carbon/nitrogen (C/N)-related and upregulation of stress-related genes. Higher expression of stress-related genes was also observed in bacteroids of stressed lpa1 plants that also displayed very high expression of the symbiotic cbb3 oxidase (fixNd). View Full-Text
Keywords: common bean; legume-rhizobia interaction; low phytic acid mutant; response to water stress; symbiotic nitrogen fixation common bean; legume-rhizobia interaction; low phytic acid mutant; response to water stress; symbiotic nitrogen fixation
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Chiozzotto, R.; Ramírez, M.; Talbi, C.; Cominelli, E.; Girard, L.; Sparvoli, F.; Hernández, G. Characterization of the Symbiotic Nitrogen-Fixing Common Bean Low Phytic Acid (lpa1) Mutant Response to Water Stress. Genes 2018, 9, 99.

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