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Volume 9
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10.3390/genes9120572
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Article

Physiological Analysis and Transcriptome Profiling of Inverted Cuttings of Populus yunnanensis Reveal That Cell Wall Metabolism Plays a Crucial Role in Responding to Inversion

by
An-Pei Zhou
1,2,
Dan Zong
1,2,
Pei-Hua Gan
1,2,
Xin-Lian Zou
1,2,
Xuan Fei
1,2,
Yuan-Yuan Zhong
1,2 and
Cheng-Zhong He
1,2,3,*
1
Key Laboratory for Forest Genetic and Tree Improvement and Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, China
2
Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, China
3
Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China
*
Author to whom correspondence should be addressed.
Genes 2018, 9(12), 572; https://doi.org/10.3390/genes9120572
Received: 15 October 2018 / Revised: 15 November 2018 / Accepted: 20 November 2018 / Published: 23 November 2018
(This article belongs to the Section Plant Genetics and Genomics)
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Abstract

Inverted cuttings of Populus yunnanensis remain alive by rooting from the original morphological apex and sprouting from the base, but the lateral branches exhibit less vigorous growth than those of the upright plant. In this study, we examined the changes in hormone contents, oxidase activities, and transcriptome profiles between upright and inverted cuttings of P. yunnanensis. The results showed that the indole-3-acetic acid (IAA) and gibberellic acid (GA3) contents were significantly lower in inverted cuttings than in upright cuttings only in the late growth period (September and October), while the abscisic acid (ABA) level was always similar between the two direction types. The biosynthesis of these hormones was surprisingly unrelated to the inversion of P. yunnanensis during the vegetative growth stage (July and August). Increased levels of peroxidases (PODs) encoded by 13 differentially expressed genes (DEGs) served as lignification promoters that protected plants against oxidative stress. Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis showed that most DEGs (107) were related to carbohydrate metabolism. Furthermore, altered activities of uridine diphosphate (UDP)-sugar pyrophosphorylase (USP, 15 DEGs) for nucleotide sugars, pectin methylesterase (PME, 7 DEGs) for pectin, and POD (13 DEGs) for lignin were important factors in the response of the trees to inversion, and these enzymes are all involved cell wall metabolism. View Full-Text
Keywords: Populus yunnanensis; inversion; cell wall; endogenous hormone; oxidase; transcriptome profiling Populus yunnanensis; inversion; cell wall; endogenous hormone; oxidase; transcriptome profiling
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MDPI and ACS Style

Zhou, A.-P.; Zong, D.; Gan, P.-H.; Zou, X.-L.; Fei, X.; Zhong, Y.-Y.; He, C.-Z. Physiological Analysis and Transcriptome Profiling of Inverted Cuttings of Populus yunnanensis Reveal That Cell Wall Metabolism Plays a Crucial Role in Responding to Inversion. Genes 2018, 9, 572. https://doi.org/10.3390/genes9120572

AMA Style

Zhou A-P, Zong D, Gan P-H, Zou X-L, Fei X, Zhong Y-Y, He C-Z. Physiological Analysis and Transcriptome Profiling of Inverted Cuttings of Populus yunnanensis Reveal That Cell Wall Metabolism Plays a Crucial Role in Responding to Inversion. Genes. 2018; 9(12):572. https://doi.org/10.3390/genes9120572

Chicago/Turabian Style

Zhou, An-Pei, Dan Zong, Pei-Hua Gan, Xin-Lian Zou, Xuan Fei, Yuan-Yuan Zhong, and Cheng-Zhong He. 2018. "Physiological Analysis and Transcriptome Profiling of Inverted Cuttings of Populus yunnanensis Reveal That Cell Wall Metabolism Plays a Crucial Role in Responding to Inversion" Genes 9, no. 12: 572. https://doi.org/10.3390/genes9120572

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