Next Article in Journal
Age-Related Epigenetic Derangement upon Reprogramming and Differentiation of Cells from the Elderly
Previous Article in Journal
Scaffolding for Repair: Understanding Molecular Functions of the SMC5/6 Complex
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Genes 2018, 9(1), 38; https://doi.org/10.3390/genes9010038

Comparative Transcriptome Analysis Identifies Putative Genes Involved in Steroid Biosynthesis in Euphorbia tirucalli

1
CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
*
Author to whom correspondence should be addressed.
Received: 17 November 2017 / Revised: 9 January 2018 / Accepted: 11 January 2018 / Published: 15 January 2018
(This article belongs to the Section Plant Genetics and Genomics)
Full-Text   |   PDF [2097 KB, uploaded 15 January 2018]   |  

Abstract

Phytochemical analysis of different Euphorbia tirucalli tissues revealed a contrasting tissue-specificity for the biosynthesis of euphol and β-sitosterol, which represent the two pharmaceutically active steroids in E. tirucalli. To uncover the molecular mechanism underlying this tissue-specificity for phytochemicals, a comprehensive E. tirucalli transcriptome derived from its root, stem, leaf and latex was constructed, and a total of 91,619 unigenes were generated with 51.08% being successfully annotated against the non-redundant (Nr) protein database. A comparison of the transcriptome from different tissues discovered members of unigenes in the upstream steps of sterol backbone biosynthesis leading to this tissue-specific sterol biosynthesis. Among them, the putative oxidosqualene cyclase (OSC) encoding genes involved in euphol synthesis were notably identified, and their expressions were significantly up-regulated in the latex. In addition, genome-wide differentially expressed genes (DEGs) in the different E. tirucalli tissues were identified. The cluster analysis of those DEGs showed a unique expression pattern in the latex compared with other tissues. The DEGs identified in this study would enrich the insights of sterol biosynthesis and the regulation mechanism of this latex-specificity. View Full-Text
Keywords: Euphorbia tirucalli; oxidosqualene cyclase; transcriptome; steroid Euphorbia tirucalli; oxidosqualene cyclase; transcriptome; steroid
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Qiao, W.; Li, C.; Mosongo, I.; Liang, Q.; Liu, M.; Wang, X. Comparative Transcriptome Analysis Identifies Putative Genes Involved in Steroid Biosynthesis in Euphorbia tirucalli. Genes 2018, 9, 38.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top