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Genes 2017, 8(12), 374; https://doi.org/10.3390/genes8120374

Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata

1
Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu City, Tokyo 183-0054, Japan
2
Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-Ku, Kyoto 603-8555, Japan
3
Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan
*
Author to whom correspondence should be addressed.
Received: 28 October 2017 / Revised: 21 November 2017 / Accepted: 30 November 2017 / Published: 8 December 2017
(This article belongs to the Special Issue Genetics and Genomics of the Rhizobium-Legume Symbiosis)
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Abstract

The establishment of a root nodule symbiosis between a leguminous plant and a rhizobium requires complex molecular interactions between the two partners. Compatible interactions lead to the formation of nitrogen-fixing nodules, however, some legumes exhibit incompatibility with specific rhizobial strains and restrict nodulation by the strains. Bradyrhizobium elkanii USDA61 is incompatible with mung bean (Vigna radiata cv. KPS1) and soybean cultivars carrying the Rj4 allele. Here, we explored genetic loci in USDA61 that determine incompatibility with V. radiata KPS1. We identified five novel B. elkanii genes that contribute to this incompatibility. Four of these genes also control incompatibility with soybean cultivars carrying the Rj4 allele, suggesting that a common mechanism underlies nodulation restriction in both legumes. The fifth gene encodes a hypothetical protein that contains a tts box in its promoter region. The tts box is conserved in genes encoding the type III secretion system (T3SS), which is known for its delivery of virulence effectors by pathogenic bacteria. These findings revealed both common and unique genes that are involved in the incompatibility of B. elkanii with mung bean and soybean. Of particular interest is the novel T3SS-related gene, which causes incompatibility specifically with mung bean cv. KPS1. View Full-Text
Keywords: symbiosis; nodulation; Bradyrhizobium elkanii; Vigna radiata; Rj4 soybean symbiosis; nodulation; Bradyrhizobium elkanii; Vigna radiata; Rj4 soybean
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Nguyen, H.P.; Miwa, H.; Kaneko, T.; Sato, S.; Okazaki, S. Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata. Genes 2017, 8, 374.

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