The Alteration of Nasopharyngeal and Oropharyngeal Microbiota in Children with MPP and Non-MPP
AbstractBackground: In recent years, the morbidity of Mycoplasma pneumoniae pneumonia (MPP) has increased significantly in China. A growing number of studies indicate that imbalanced respiratory microbiota is associated with various respiratory diseases. Methods: We enrolled 119 children, including 60 pneumonia patients and 59 healthy children. Nasopharyngeal (NP) and oropharyngeal (OP) sampling was performed for 16S ribosomal RNA (16S rRNA) gene analysis of all children. Sputum and OP swabs were obtained from patients for pathogen detection. Results: Both the NP and OP microbiota of patients differ significantly from that of healthy children. Diseased children harbor lower microbial diversity and a simpler co-occurrence network in NP and OP. In pneumonia patients, NP and OP microbiota showed greater similarities between each other, suggesting transmission of NP microbiota to the OP. Aside from clinically detected pathogens, NP and OP microbiota analysis has also identified possible pathogens in seven cases with unknown infections. Conclusion: NP and OP microbiota in MPP and non-MPP are definitely similar. Respiratory infection generates imbalanced NP microbiota, which has the potential to transmit to OP. Microbiota analysis also promises to compliment the present means of detecting respiratory pathogens. View Full-Text
- Supplementary File 1:
Supplementary (XLSX, 133 KB)
Share & Cite This Article
Lu, Z.; Dai, W.; Liu, Y.; Zhou, Q.; Wang, H.; Li, D.; Yang, Z.; Li, Y.; Xie, G.; Li, S.; Zheng, Y. The Alteration of Nasopharyngeal and Oropharyngeal Microbiota in Children with MPP and Non-MPP. Genes 2017, 8, 380.
Lu Z, Dai W, Liu Y, Zhou Q, Wang H, Li D, Yang Z, Li Y, Xie G, Li S, Zheng Y. The Alteration of Nasopharyngeal and Oropharyngeal Microbiota in Children with MPP and Non-MPP. Genes. 2017; 8(12):380.Chicago/Turabian Style
Lu, Zhiwei; Dai, Wenkui; Liu, Yanhong; Zhou, Qian; Wang, Heping; Li, Dongfang; Yang, Zhenyu; Li, Yinhu; Xie, Gan; Li, Shuaicheng; Zheng, Yuejie. 2017. "The Alteration of Nasopharyngeal and Oropharyngeal Microbiota in Children with MPP and Non-MPP." Genes 8, no. 12: 380.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.