The Transcriptional Coactivator DEAD/H Box 5 (DDX5) Gene Is a Target of the Transcription Factor E2F1 Deregulated from the Tumor Suppressor pRB
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Plasmids
2.3. Transfection and Reporter Assay
2.4. Infection with Recombinant Adenovirus
2.5. Quantitative Reverse Transcription (qRT)-PCR
2.6. Immunoblot Analysis
2.7. Chromatin Immunoprecipitation (ChIP) Assay
2.8. Statistical Analysis
3. Results
3.1. Deregulated E2F1 and Growth Stimulation Induced DDX5 Gene Expression in Human Normal Fibroblasts
3.2. Over-Expression of E2F1 Activates DDX5 Promoter
3.3. Physiological E2F1 Induced by Growth-Stimulation Does Not Activate DDX5 Promoter
3.4. The DDX5 Gene Is a Target of Deregulated E2F1
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Nakajima, R.; Zhou, Y.; Shirasawa, M.; Fikriyanti, M.; Iwanaga, R.; Bradford, A.P.; Kurayoshi, K.; Araki, K.; Ohtani, K. The Transcriptional Coactivator DEAD/H Box 5 (DDX5) Gene Is a Target of the Transcription Factor E2F1 Deregulated from the Tumor Suppressor pRB. Genes 2025, 16, 929. https://doi.org/10.3390/genes16080929
Nakajima R, Zhou Y, Shirasawa M, Fikriyanti M, Iwanaga R, Bradford AP, Kurayoshi K, Araki K, Ohtani K. The Transcriptional Coactivator DEAD/H Box 5 (DDX5) Gene Is a Target of the Transcription Factor E2F1 Deregulated from the Tumor Suppressor pRB. Genes. 2025; 16(8):929. https://doi.org/10.3390/genes16080929
Chicago/Turabian StyleNakajima, Rinka, Yaxuan Zhou, Mashiro Shirasawa, Mariana Fikriyanti, Ritsuko Iwanaga, Andrew P. Bradford, Kenta Kurayoshi, Keigo Araki, and Kiyoshi Ohtani. 2025. "The Transcriptional Coactivator DEAD/H Box 5 (DDX5) Gene Is a Target of the Transcription Factor E2F1 Deregulated from the Tumor Suppressor pRB" Genes 16, no. 8: 929. https://doi.org/10.3390/genes16080929
APA StyleNakajima, R., Zhou, Y., Shirasawa, M., Fikriyanti, M., Iwanaga, R., Bradford, A. P., Kurayoshi, K., Araki, K., & Ohtani, K. (2025). The Transcriptional Coactivator DEAD/H Box 5 (DDX5) Gene Is a Target of the Transcription Factor E2F1 Deregulated from the Tumor Suppressor pRB. Genes, 16(8), 929. https://doi.org/10.3390/genes16080929