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Volume 16
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10.3390/genes16040368
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Article

Responses of the Lipoxygenase Gene Family to Drought Stress in Broomcorn Millet (Panicum miliaceum L.)

by
Lin Cong
1,
Lin Deng
1,
Hongfei Yao
1,
Yaoyuan Zhang
1,
Hongying Li
1,
Haigang Wang
2,
Bin Zhang
1,3,
Yuanhuai Han
1,3,* and
Junjie Wang
1,*
1
College of Agriculture, Shanxi Agricultural University, Taigu 030801, China
2
Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan 030031, China
3
Houji Laboratory in Shanxi Province, Taiyuan 030031, China
*
Authors to whom correspondence should be addressed.
Genes 2025, 16(4), 368; https://doi.org/10.3390/genes16040368
Submission received: 26 February 2025 / Revised: 19 March 2025 / Accepted: 20 March 2025 / Published: 23 March 2025
(This article belongs to the Section Bioinformatics)
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Abstract

Background: Broomcorn millet (Panicum miliaceum L.), a drought-tolerant C4 crop, is crucial for agricultural resilience in arid regions. Lipoxygenases (LOXs), key enzymes in plant stress responses, have not been studied in broomcorn millet. This study aimed to identify LOX genes in broomcorn millet and elucidate their role in drought tolerance. Methods: We employed bioinformatics and physiological analyses to identify LOX genes in broomcorn millet. Expression profiles were assessed in different organs, and drought stress responses were evaluated in tolerant (HSZ, YXDHM) and sensitive (YS10) varieties. Antioxidant enzyme activities (SOD, POD, CAT) and malondialdehyde (MDA) levels were measured. Results: Twelve LOX genes were identified, classified into three subfamilies, and mapped across seven chromosomes. These genes contained stress-responsive cis-elements and showed organ-specific expression, with PmLOX5 exhibiting no detectable expression. Under drought stress, tolerant varieties showed elevated antioxidant activities and reduced MDA accumulation. PmLOX2, a homolog of Arabidopsis AtLOX1/AtLOX5, was significantly induced in tolerant varieties, correlating with enhanced antioxidant capacity and reduced oxidative damage. Conclusions:PmLOX genes, particularly PmLOX2, play a pivotal role in drought tolerance by modulating ROS scavenging and membrane protection. This study provides a foundation for leveraging LOX genes to improve drought resilience in broomcorn millet and related crops.
Keywords: broomcorn millet; LOX gene family; drought stress; gene expression; abiotic stress adaptation broomcorn millet; LOX gene family; drought stress; gene expression; abiotic stress adaptation

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MDPI and ACS Style

Cong, L.; Deng, L.; Yao, H.; Zhang, Y.; Li, H.; Wang, H.; Zhang, B.; Han, Y.; Wang, J. Responses of the Lipoxygenase Gene Family to Drought Stress in Broomcorn Millet (Panicum miliaceum L.). Genes 2025, 16, 368. https://doi.org/10.3390/genes16040368

AMA Style

Cong L, Deng L, Yao H, Zhang Y, Li H, Wang H, Zhang B, Han Y, Wang J. Responses of the Lipoxygenase Gene Family to Drought Stress in Broomcorn Millet (Panicum miliaceum L.). Genes. 2025; 16(4):368. https://doi.org/10.3390/genes16040368

Chicago/Turabian Style

Cong, Lin, Lin Deng, Hongfei Yao, Yaoyuan Zhang, Hongying Li, Haigang Wang, Bin Zhang, Yuanhuai Han, and Junjie Wang. 2025. "Responses of the Lipoxygenase Gene Family to Drought Stress in Broomcorn Millet (Panicum miliaceum L.)" Genes 16, no. 4: 368. https://doi.org/10.3390/genes16040368

APA Style

Cong, L., Deng, L., Yao, H., Zhang, Y., Li, H., Wang, H., Zhang, B., Han, Y., & Wang, J. (2025). Responses of the Lipoxygenase Gene Family to Drought Stress in Broomcorn Millet (Panicum miliaceum L.). Genes, 16(4), 368. https://doi.org/10.3390/genes16040368

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