Identification of a Rye Spring Mutant Derived from a Winter Rye Variety by High-Altitude Environment Screening Using RNA Sequencing Technology
Abstract
:1. Introduction
2. Materials and Methods
2.1. Test Site Profile
2.2. Experimental Materials
2.3. Field Conditions
2.4. Content and Method of Determination
3. Results
3.1. The Manipulation of Sowing Dates Significantly Influenced the Forage Yield of Rye
3.2. The Identification of Winter–Spring Type Indicated That Ganyin No1 Rye Is a Spring Type
3.3. Differential Gene Expression Analysis
3.4. The GO Annotation and Enrichment Analysis of Differentially Expressed Genes
3.5. Differential Gene Screening Identified Genes Associated with the Vernalization Process
3.6. Differential Gene Screening to Genes Associated with the Photoperiodic
3.7. The KEGG Annotation and Enrichment Analysis of Differentially Expressed Genes
3.8. KEGG Enrichment Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Planting Season | Species Name | Growth Pattern | Seedling-Heading Period/d | Heading Rate/% |
---|---|---|---|---|
18 February | Wintergrazer-70 | 3 | 69 | 1.3 |
Ganyin No1 | 3 | 42 | 63.4 | |
25 February | Wintergrazer-70 | 3 | 67 | 0.9 |
Ganyin No1 | 3 | 42 | 61.8 | |
4 March | Wintergrazer-70 | 3 | 68 | 0.8 |
Ganyin No1 | 3 | 41 | 62.7 | |
11 March | Wintergrazer-70 | 3 | 68 | 1.1 |
Ganyin No1 | 3 | 42 | 63.2 | |
5 March | Wintergrazer-70 | 3 | 67 | 1 |
Ganyin No1 | 3 | 41 | 63.8 | |
19 March | Wintergrazer-70 | 3 | ||
Ganyin No1 | 3 | 40 | 63.1 | |
23 March | Wintergrazer-70 | 3 | ||
Ganyin No1 | 3 | 40 | 61.3 | |
27 March | Wintergrazer-70 | 3 | ||
Ganyin No1 | 3 | 40 | 62.9 |
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Wang, Y.; Liu, Y.; Yu, C.; Chen, S.; Li, Y.; Wei, L.; Wu, J.; Yang, J. Identification of a Rye Spring Mutant Derived from a Winter Rye Variety by High-Altitude Environment Screening Using RNA Sequencing Technology. Genes 2024, 15, 572. https://doi.org/10.3390/genes15050572
Wang Y, Liu Y, Yu C, Chen S, Li Y, Wei L, Wu J, Yang J. Identification of a Rye Spring Mutant Derived from a Winter Rye Variety by High-Altitude Environment Screening Using RNA Sequencing Technology. Genes. 2024; 15(5):572. https://doi.org/10.3390/genes15050572
Chicago/Turabian StyleWang, Yanying, Yixuan Liu, Chengqun Yu, Shizhan Chen, Yankun Li, Lina Wei, Junxi Wu, and Jianping Yang. 2024. "Identification of a Rye Spring Mutant Derived from a Winter Rye Variety by High-Altitude Environment Screening Using RNA Sequencing Technology" Genes 15, no. 5: 572. https://doi.org/10.3390/genes15050572