Genome-Wide Association Studies Revealed Several Candidate Genes of Meat Productivity in Saryarka Fat-Tailed Coarse-Wool Sheep Breed
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Animals and Phenotypic Data Collection
2.2. DNA Extraction and Quality Control
2.3. SNP Genotyping and Quality Control
2.4. Genome-Wide Association Studies and Candidate Gene Annotation
3. Results
3.1. Phenotypic Data
3.2. SNP Quality Control Results
3.3. Genome-Wide Association Analysis and Gene Annotation Results
3.3.1. Live Weight
3.3.2. Rump Height
3.3.3. Bicoastal Diameter
3.3.4. Body Depth
3.3.5. Body Length
3.3.6. Rump Width
3.3.7. Heart Girth
3.3.8. Cannon Bone Circumference
4. Discussion
4.1. Live Weight
4.2. Bicoastal Diameter
4.3. Rump Width and Cannon Bone Circumference
4.4. Heart Girth
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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№ | Physical Characteristics | Description |
---|---|---|
1 | Live weight | The weight of an animal after completion of rearing and feeding |
2 | Wither height | Determined by the measurement of the highest point of the withers in a straight line from the ground |
3 | Rump height | Determined by the measurement of the highest point of the sacrum |
4 | Bicoastal diameter | Determined by the measurement of the widest point vertically, tangent to the rear angle of the scapula |
5 | Body depth | Determined by the measurement of the vertical distance from the highest point of the withers behind the shoulder blades to the lower surface of the chest |
6 | Body length | Determined by the measurement of the distance from the extreme anterior point of the scapulohumeral joint to the inner protrusion of the ischial tuberosity |
7 | Rump width | Measured as the distance between the most posterior point of the pin bones |
8 | Heart girth | Determined by the measurement around the circumference, passing tangent to the back angle of the shoulder blade |
9 | Cannon bone circumference | Determined by the measurement of the girth at the thinnest point of the metacarpal bone |
№ | Physical Characteristics | Sample Size | Mean | Min. Value | Max. Value |
---|---|---|---|---|---|
1 | LW, kg | 100 | 50.601 ± 3.924 | 42.8 | 58.1 |
2 | WH, cm | 100 | 71.205 ± 1.432 | 67.0 | 73.8 |
3 | RH, cm | 100 | 71.816 ± 1.778 | 65.0 | 74.2 |
4 | BCD, cm | 100 | 21.95 ± 1.579 | 18.0 | 24.0 |
5 | BD, cm | 100 | 30.69 ± 1.846 | 25.0 | 35.0 |
6 | BL, cm | 100 | 82.96 ± 3.843 | 72.0 | 90.0 |
7 | RW, cm | 100 | 22.55 ± 2.143 | 19.0 | 27.0 |
8 | HG, cm | 100 | 94.57 ± 4.115 | 86.0 | 102.0 |
9 | CBG, cm | 100 | 9.354 ± 0.893 | 8.0 | 11.0 |
№ | Phenotypic Characteristics | Chr | SNP in Gene | Candidate Genes | Related SNPs | Position, bp | Substitution | p-Value | Association Level |
---|---|---|---|---|---|---|---|---|---|
1 | LW | 4 | No | - | s38034.1 | 76916480 | T<C | 0.00007048 | Suggestive |
6 | No | - | OAR6_96649415.1 | 88113526 | A<G | 0.00007065 | Suggestive | ||
5 | No | - | OAR5_95756483.1 | 87568490 | G<A | 0.00007584 | Suggestive | ||
3 | Yes | IGFBP6 | s20793.1 | 133214823 | C<T | 0.001312 | Suggestive | ||
2 | WH | No significant and suggestive SNPs were found | |||||||
3 | RH | 1 | No | - | OAR1_155107985.1 | 143433100 | T<C | 0.0001638 | Suggestive |
17 | No | - | OAR17_39581977.1 | 36612240 | C<T | 0.0002826 | Suggestive | ||
4 | BCD | 15 | No | - | s59091.1 | 64420672 | G<T | 0.0001018 | Suggestive |
4 | Yes | ST7 | OAR4_54217431.1 | 51203290 | C<T | 0.0004799 | Suggestive | ||
5 | BD | 9 | No | - | s55235.1 | 42238463 | G<A | 0.000018 | Suggestive |
6 | BL | 5 | No | - | OAR5_114683952_X.1 | 105265869 | C<A | 0.0000171 | Suggestive |
2 | No | - | OAR2_40125657.1 | 38578195 | T<C | 0.00002798 | Suggestive | ||
3 | No | - | s06635.1 | 37310135 | C<T | 0.00004734 | Suggestive | ||
7 | RW | 22 | - | - | s39884.1 | 27144608 | A<G | 0.00000000115 | Significant |
9 | - | - | OAR9_71374559.1 | 67241520 | G<A | 0.0000000254 | Significant | ||
4 | - | - | OAR4_65928409.1 | 62315843 | G<A | 0.000000277 | Significant | ||
2 | - | - | OAR2_199679972.1 | 188291939 | A<G | 0.00000318 | Significant | ||
6 | No | - | OAR6_20647551.1 | 17680070 | G<A | 0.00000952 | Significant | ||
6 | Yes | SCD5 | s25229.1 | 97139005 | A<G | 0.0004653 | Suggestive | ||
20 | Yes | DTNBP1 | s01175.1 | 40381688 | T<C | 0.002427 | Suggestive | ||
8 | HG | 5 | No | - | s55928.1 | 45763719 | C<T | 0.000000000993 | Significant |
7 | - | - | OAR7_11868747.1 | 11579221 | A<G | 0.00000000106 | Significant | ||
2 | Yes | KYNU | OAR2_175574781.1 | 165981432 | A<G | 0.0000000011 | Significant | ||
2 | No | - | OAR2_165244995.1 | 155873954 | A<G | 0.0000000266 | Significant | ||
12 | No | - | OAR12_37292970.1 | 33220261 | A<G | 0.0000000277 | Significant | ||
21 | No | - | OAR21_33775912_X.1 | 30148681 | G<T | 0.000000256 | Significant | ||
18 | - | - | OAR18_11476519.1 | 11480063 | G<A | 0.00000288 | Significant | ||
2 | - | - | OAR2_48251746.1 | 45537012 | C<T | 0.00000678 | Significant | ||
9 | CBG | 8 | - | - | OAR8_42165866.1 | 39156805 | C<T | 0.000007901 | Significant |
8 | No | - | OAR8_25841996.1 | 23361179 | T<G | 0.00002434 | Suggestive | ||
1 | Yes | FGF12 | OAR1_209022621.1 | 193436737 | T<C | 0.0001194 | Suggestive | ||
17 | No | - | s49020.1 | 65960779 | A<C | 0.0001891 | Suggestive | ||
14 | Yes | FTO | s15415.1 | 21730916 | A<C | 0.003363 | Suggestive |
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Dossybayev, K.; Amandykova, M.; Orakbayeva, A.; Adylkanova, S.; Kozhakhmet, A.; Yergali, K.; Kulboldin, T.; Kulataev, B.; Torekhanov, A. Genome-Wide Association Studies Revealed Several Candidate Genes of Meat Productivity in Saryarka Fat-Tailed Coarse-Wool Sheep Breed. Genes 2024, 15, 1549. https://doi.org/10.3390/genes15121549
Dossybayev K, Amandykova M, Orakbayeva A, Adylkanova S, Kozhakhmet A, Yergali K, Kulboldin T, Kulataev B, Torekhanov A. Genome-Wide Association Studies Revealed Several Candidate Genes of Meat Productivity in Saryarka Fat-Tailed Coarse-Wool Sheep Breed. Genes. 2024; 15(12):1549. https://doi.org/10.3390/genes15121549
Chicago/Turabian StyleDossybayev, Kairat, Makpal Amandykova, Ainur Orakbayeva, Sholpan Adylkanova, Altynay Kozhakhmet, Kanagat Yergali, Temirlan Kulboldin, Beibit Kulataev, and Aibyn Torekhanov. 2024. "Genome-Wide Association Studies Revealed Several Candidate Genes of Meat Productivity in Saryarka Fat-Tailed Coarse-Wool Sheep Breed" Genes 15, no. 12: 1549. https://doi.org/10.3390/genes15121549
APA StyleDossybayev, K., Amandykova, M., Orakbayeva, A., Adylkanova, S., Kozhakhmet, A., Yergali, K., Kulboldin, T., Kulataev, B., & Torekhanov, A. (2024). Genome-Wide Association Studies Revealed Several Candidate Genes of Meat Productivity in Saryarka Fat-Tailed Coarse-Wool Sheep Breed. Genes, 15(12), 1549. https://doi.org/10.3390/genes15121549