B Chromosome Transcriptional Inactivation in the Spermatogenesis of the Grasshopper Eyprepocnemis plorans
Abstract
:1. Introduction
2. Materials and Methods
2.1. Spreading and Squashing of Seminiferous Tubules
2.2. Immunofluorescence Microscopy
2.3. Image Acquisition and Processing
2.4. RAD51 Foci Quantification
2.5. Fluorescence Quantification
2.6. Electron Microscopy
3. Results
3.1. Meiotic Behavior of the B1 Chromosome
3.2. Cohesin Subunit SMC3 Immunolabeling as a Marker for Chromosome Pairing and Synapsis Throughout Prophase I Stages
3.3. B1 Bivalent Is Chiasmatic
3.4. Transcriptional Activity of B1 Chromosomes Does Not Depend on Their Univalent or Bivalent Condition
3.5. Transcriptional Inactivation of B1 Chromosome Initiates in Spermatogonia
4. Discussion
4.1. B1 Chromosomes Remain Inactive During Prophase I Regardless of Whether They Complete Synapsis or Not
4.2. X and B1 Chromosomes Enter Meiosis in a Pre-Inactivated State
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Santos, J.L.; Parra, M.T.; Arévalo, S.; Guajardo-Grence, A.; Page, J.; Suja, J.Á.; García de la Vega, C.; Viera, A. B Chromosome Transcriptional Inactivation in the Spermatogenesis of the Grasshopper Eyprepocnemis plorans. Genes 2024, 15, 1512. https://doi.org/10.3390/genes15121512
Santos JL, Parra MT, Arévalo S, Guajardo-Grence A, Page J, Suja JÁ, García de la Vega C, Viera A. B Chromosome Transcriptional Inactivation in the Spermatogenesis of the Grasshopper Eyprepocnemis plorans. Genes. 2024; 15(12):1512. https://doi.org/10.3390/genes15121512
Chicago/Turabian StyleSantos, Juan Luis, María Teresa Parra, Sara Arévalo, Andrea Guajardo-Grence, Jesús Page, José Ángel Suja, Carlos García de la Vega, and Alberto Viera. 2024. "B Chromosome Transcriptional Inactivation in the Spermatogenesis of the Grasshopper Eyprepocnemis plorans" Genes 15, no. 12: 1512. https://doi.org/10.3390/genes15121512
APA StyleSantos, J. L., Parra, M. T., Arévalo, S., Guajardo-Grence, A., Page, J., Suja, J. Á., García de la Vega, C., & Viera, A. (2024). B Chromosome Transcriptional Inactivation in the Spermatogenesis of the Grasshopper Eyprepocnemis plorans. Genes, 15(12), 1512. https://doi.org/10.3390/genes15121512