Proteomics Analysis to Explore the Resistance Genes of Silkworm to Bombyx mori Nuclear Polyhedrosis Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Silkworm Varieties and Virus Strains
2.2. Virus Inoculation and Sampling
2.3. Detection of BmNPV Proliferation in the Hemolymph
2.4. Protein Extraction and 2-DE
2.5. RNA Extraction and qRT-PCR
3. Results
3.1. Midgut Protein of Su Was Degraded Greatly after BmNPV Inoculation
3.2. BmNPV Proliferated Rapidly in the Hemolymph of Su after Infection
3.3. Expression of Hemolymph Proteins in Su and SuN after BmNPV Inoculation
3.4. Transcriptional Level Analysis of Genes Corresponding to DEPs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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DEPs | Genes | Chromosome Position | Gene Annotation in Silkworm Genome Database |
---|---|---|---|
S1 | KWMTBOMO13811 | Chr23: 10000380–10005184 (+) | hemolymph juvenile hormone binding protein precursor [Bombyx mori] |
S2 | KWMTBOMO03739 | Chr7: 371497–372518 (−) | Uncharacterized protein [Bombyx mori] |
S3 | KWMTBOMO03745 | Chr7: 422709–423596 (+) | Uncharacterized protein [Bombyx mori] |
SN1 | KWMTBOMO08187 | Chr14: 1161961–1192896 (+) | Venom acid phosphatase Acph-1-like [Papilio xuthus] |
SN2 | KWMTBOMO06255 | Chr11: 3382416–3398048 (+) | Probable polyamine oxidase 5 [Bombyx mori] |
SN3 | KWMTBOMO13992 | Chr23: 15551943–15556861 (−) | sex-specific storage-protein 1 precursor [Bombyx mori] |
SN4 | KWMTBOMO09392 | Chr16: 360567–373088 (+) | Pro-phenol oxidase [Bombyx mandarina] |
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Ouyang, G.; Qian, H.; Sun, J.; Yang, R.; Gui, T.; Wang, W.; Liu, Q.; Chen, A. Proteomics Analysis to Explore the Resistance Genes of Silkworm to Bombyx mori Nuclear Polyhedrosis Virus. Genes 2024, 15, 59. https://doi.org/10.3390/genes15010059
Ouyang G, Qian H, Sun J, Yang R, Gui T, Wang W, Liu Q, Chen A. Proteomics Analysis to Explore the Resistance Genes of Silkworm to Bombyx mori Nuclear Polyhedrosis Virus. Genes. 2024; 15(1):59. https://doi.org/10.3390/genes15010059
Chicago/Turabian StyleOuyang, Gui, Heying Qian, Juan Sun, Runhuan Yang, Tao Gui, Wenbing Wang, Qiang Liu, and Anli Chen. 2024. "Proteomics Analysis to Explore the Resistance Genes of Silkworm to Bombyx mori Nuclear Polyhedrosis Virus" Genes 15, no. 1: 59. https://doi.org/10.3390/genes15010059
APA StyleOuyang, G., Qian, H., Sun, J., Yang, R., Gui, T., Wang, W., Liu, Q., & Chen, A. (2024). Proteomics Analysis to Explore the Resistance Genes of Silkworm to Bombyx mori Nuclear Polyhedrosis Virus. Genes, 15(1), 59. https://doi.org/10.3390/genes15010059