Long Non-Coding RNA SNHG4 Expression in Women with Endometriosis: A Pilot Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients
2.2. RNA Isolation
2.3. Spectrophotometric Analysis of Purity and RNA Concentration
2.4. RT-PCR Methods
2.5. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Patients (n = 100) | Control (n = 100) | ||
---|---|---|---|
Age (Range) 21–53 Years Age (Mean) 34.89 ± 7.49 | Age (Range) 26–67 Years Age (Mean) 47.93 ± 6.01 | ||
BMI (kg/m2) | The number (%) | BMI (kg/m2) | The number (%) |
<25 | 65 (65%) | <25 | 55 (55%) |
25 ≤ BMI < 30 | 25 (25%) | 25 ≤ BMI < 30 | 24 (24%) |
≥30 | 10 (10%) | ≥30 | 21 (21%) |
Parity | The number (%) | Parity | The number (%) |
yes | 45 (45%) | yes | 50 (50%) |
−1 | 20 (20%) | −1 | 25 (25%) |
−2 and more | 24 (24%) | −2 and more | 20 (20%) |
no | 11 (11%) | no | 5 (5%) |
Spontaneous abortions | The number (%) | Spontaneous abortions | The number (%) |
yes (spontaneous) | 6 (6%) | yes (spontaneous) | 10 (10%) |
no | 94 (94%) | no | 90 (90%) |
Clinical stage | The number (%) | ||
I | 24 (24%) | ||
II | 27 (27%) | ||
III | 18 (18%) | ||
IV | 31 (31%) |
SNHG4 | Test Material | Median | Percentile 25 | Percentile 75 | pa |
---|---|---|---|---|---|
endometriosis | 203.92 | 170.48 | 1011.22 | <0.050 | |
control | 201.878 | 168.74 | 768.64 |
Mann-Whitney Test | ||
---|---|---|
p = 0.78 | ||
Parity = 0 | Parity > 0 | |
number of preparations | 38 | 62 |
average ± SD | 214.3± 186.0 | 206.1 ± 154.3 |
Mann-Whitney Test | ||
---|---|---|
p = 0.031 | ||
rASRM 1–2 | rASRM 3–4 | |
number of preparations | 100 | 100 |
min. | 126.67 | 128.10 |
max | 670.11 | 2180.12 |
average ± SD | 398.3 ± 186.0 | 1154.1 ± 154.3 |
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Szaflik, T.; Romanowicz, H.; Szyłło, K.; Smolarz, B. Long Non-Coding RNA SNHG4 Expression in Women with Endometriosis: A Pilot Study. Genes 2023, 14, 152. https://doi.org/10.3390/genes14010152
Szaflik T, Romanowicz H, Szyłło K, Smolarz B. Long Non-Coding RNA SNHG4 Expression in Women with Endometriosis: A Pilot Study. Genes. 2023; 14(1):152. https://doi.org/10.3390/genes14010152
Chicago/Turabian StyleSzaflik, Tomasz, Hanna Romanowicz, Krzysztof Szyłło, and Beata Smolarz. 2023. "Long Non-Coding RNA SNHG4 Expression in Women with Endometriosis: A Pilot Study" Genes 14, no. 1: 152. https://doi.org/10.3390/genes14010152
APA StyleSzaflik, T., Romanowicz, H., Szyłło, K., & Smolarz, B. (2023). Long Non-Coding RNA SNHG4 Expression in Women with Endometriosis: A Pilot Study. Genes, 14(1), 152. https://doi.org/10.3390/genes14010152