Single Nucleotide Polymorphisms of ALDH18A1 and MAT2A Genes and Their Genetic Associations with Milk Production Traits of Chinese Holstein Cows
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Selection, Pedigree, and Phenotypic Data Collation
2.2. Genomic DNA Extraction from Frozen Semen and Blood Samples
2.3. Polymorphism Detection of Candidate Genes
2.4. Linkage Disequilibrium (LD) Estimation
2.5. Association Analysis of Single Marker/Haplotype and Milk Production Traits
2.6. Biological Function Prediction
3. Results
3.1. SNPs Identification
3.2. Single Marker Association Analysis
3.3. Haplotype Association Analysis
3.4. Functional Variation Prediction Caused by SNPs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | SNP Name | RS ID | Position (UMD3.1) | Gene Region | Genotype | Genotypic Frequency | Allele | Allelic Frequency | Hardy–Weinberg Equilibrium |
---|---|---|---|---|---|---|---|---|---|
ALDH18A1 | 26:g.17130318C>A | rs109124430 | Chr26: | 5′ flanking region | AA | 0.6840 | A | 0.8274 | T |
17130318 | AC | 0.2868 | C | 0.1726 | |||||
CC | 0.0292 | ||||||||
26:g.17118244G>A | rs133295794 | Chr26: | Exon (synonymous) | AA | 0.0195 | A | 0.1239 | T | |
17118244 | AG | 0.2089 | G | 0.8761 | |||||
GG | 0.7716 | ||||||||
26:g.17102977T>C | rs136218403 | Chr26: | Intron | CC | 0.0216 | C | 0.1261 | T | |
17102977 | CT | 0.2089 | T | 0.8739 | |||||
TT | 0.7695 | ||||||||
26:g.17100534G>T | rs110706194 | Chr26: | Exon (synonymous) | GG | 0.8680 | G | 0.9324 | T | |
17100534 | GT | 0.1288 | T | 0.0676 | |||||
TT | 0.0032 | ||||||||
26:g.17089560G>A | rs458180458 | Chr26: | Exon (synonymous) | AA | 0.0119 | A | 0.0947 | T | |
17089560 | AG | 0.1656 | G | 0.9053 | |||||
GG | 0.8225 | ||||||||
26:g.17088978A>G | rs41255559 | Chr26: | 3′UTR (untranslated region) | AA | 0.0606 | A | 0.2262 | T | |
17088978 | AG | 0.3312 | G | 0.7738 | |||||
GG | 0.6082 | ||||||||
26:g.17088098G>C | rs208352883 | Chr26: | 3′ flanking region | CC | 0.3506 | C | 0.5812 | T | |
17088098 | CG | 0.4610 | G | 0.4188 | |||||
GG | 0.1883 | ||||||||
26:g.17086802T>A | rs109201383 | Chr26: | 3′ flanking region | AA | 0.3712 | A | 0.6012 | T | |
17086802 | AT | 0.4600 | T | 0.3988 | |||||
TT | 0.1688 | ||||||||
MAT2A | 11:g.49472723G>C | rs109079969 | Chr26: | 5′ flanking region | CC | 0.6396 | C | 0.8003 | T |
49472723 | CG | 0.3214 | G | 0.1997 | |||||
GG | 0.0390 | ||||||||
11:g.49465032C>T | rs110124316 | Chr26: | 3′ flanking region | CC | 0.4913 | C | 0.6813 | F | |
49465032 | CT | 0.3798 | T | 0.3187 | |||||
TT | 0.1288 |
Genes | SNPs | Lactation | Genotype (No.) | Milk Yield (kg) | Fat Yield (kg) | Fat Percentage (%) | Protein Yield (kg) | Protein Percentage (%) |
---|---|---|---|---|---|---|---|---|
ALDH18A1 | 26:g.17130318C>A | 1 | AA (632) | 10,312 ± 59.78 | 339.4 ± 2.66 | 3.31 ± 0.02 | 305.9 ± 1.94 | 2.98 ± 0.02 |
AC (265) | 10,145 ± 69.33 | 338.84 ± 3.01 | 3.37 ± 0.03 | 302.59 ± 2.19 | 3.00 ± 0.02 | |||
CC (27) | 10,211 ± 149.06 a | 339.89 ± 6.11 | 3.36 ± 0.06 | 305.07 ± 4.45 | 3.00 ± 0.04 | |||
8.76 | 0.07 | 6.86 | 3.92 | 2.15 | ||||
p value | 0.0128 | 0.8714 | 0.0329 | 0.1405 | 0.3414 | |||
Corrected p | 0.1024 | 6.9712 | 0.2632 | 1.124 | 2.7312 | |||
2 | AA (437) | 10,850 ± 61.29 | 388.69 ± 2.73 Aa | 3.59 ± 0.03 | 322 ± 1.99 Aa | 2.97 ± 0.02 | ||
AC (181) | 10,694 ± 76.68 | 383.71 ± 3.31 Aa | 3.59 ± 0.03 | 315.51 ± 2.41 ABb | 2.95 ± 0.02 | |||
CC (17) | 10,331 ± 194.55 | 349.48 ± 7.96 Bb | 3.40 ± 0.08 | 299.99 ± 5.80 Bb | 2.91 ± 0.05 | |||
10.81 | 26.7 | 6.09 | 22.03 | 3.14 | ||||
p value | 0.0047 | <0.0001 | 0.0485 | <0.0001 | 0.2088 | |||
Corrected p | 0.0376 | <0.0001 | 0.388 | <0.0001 | 1.6704 | |||
26:g.17118244G>A | 1 | AA (18) | 10,232 ± 179.35 | 344.82 ± 7.31 | 3.39 ± 0.07 | 304.58 ± 5.33 | 2.99 ± 0.04 | |
AG (193) | 10,292 ± 75.23 | 342.37 ± 3.24 | 3.36 ± 0.03 | 306.49 ± 2.36 | 3.00 ± 0.02 | |||
GG (713) | 10,251 ± 58.52 | 338.26 ± 2.62 | 3.32 ± 0.02 | 304.47 ± 1.90 | 2.98 ± 0.02 | |||
0.46 | 3.36 | 2.64 | 1.24 | 0.76 | ||||
p value | 0.796 | 0.187 | 0.2675 | 0.5381 | 0.6851 | |||
Corrected p | 6.368 | 1.496 | 2.14 | 4.3048 | 5.4808 | |||
2 | AA (12) | 10,527 ± 227.41 ab | 362.45 ± 9.26 a | 3.46 ± 0.09 | 307.38 ± 6.75 | 2.93 ± 0.05 | ||
AG (133) | 10,997 ± 84.80 a | 391.85 ± 3.63 b | 3.57 ± 0.03 | 325.40 ± 2.64 | 2.96 ± 0.02 | |||
GG (490) | 10,751 ± 60.15 b | 385.76 ± 2.69 ab | 3.59 ± 0.02 | 318.67 ± 1.96 | 2.97 ± 0.02 | |||
11.76 | 11.5 | 2.25 | 12.73 | 0.71 | ||||
p value | 0.003 | 0.0034 | 0.3248 | 0.0019 | 0.7024 | |||
Corrected p | 0.024 | 0.0272 | 2.5984 | 0.0152 | 5.6192 | |||
26:g.17102977T>C | 1 | CC (20) | 10,240 ± 171.14 | 343.5 ± 6.98 | 3.37 ± 0.07 | 305.74 ± 5.09 | 3.00 ± 0.04 | |
CT (193) | 10,278 ± 75.15 | 342.12 ± 3.23 | 3.36 ± 0.03 | 306 ± 2.36 | 3.00 ± 0.02 | |||
TT (711) | 10,255 ± 58.58 | 338.33 ± 2.62 | 3.32 ± 0.02 | 304.55 ± 1.91 | 2.98 ± 0.02 | |||
0.16 | 2.73 | 2.72 | 0.67 | 0.8 | ||||
p value | 0.9253 | 0.2564 | 0.2577 | 0.7137 | 0.6691 | |||
Corrected p | 7.4024 | 2.0512 | 2.0616 | 5.7096 | 5.3528 | |||
2 | CC (12) | 10,524 ± 227.4 | 362.32 ± 9.26 | 3.46 ± 0.09 | 307.29 ± 6.75 | 2.93 ± 0.05 | ||
CT (134) | 10,978 ± 84.37 | 390.97 ± 3.61 | 3.57 ± 0.03 | 324.86 ± 2.63 | 2.96 ± 0.02 | |||
TT (489) | 10,755 ± 60.20 | 385.96 ± 2.69 B | 3.59 ± 0.02 | 318.78 ± 1.96 | 2.97 ± 0.02 | |||
10.11 | 10.31 | 2.45 | 11.22 | 0.71 | ||||
p value | 0.0067 | 0.0061 | 0.2946 | 0.0039 | 0.7021 | |||
Corrected p | 0.0536 | 0.0488 | 2.3568 | 0.0312 | 5.6168 | |||
26:g.17100534G>T | 1 | GG (802) | 10,306 ± 58.10 Aa | 340.22 ± 2.60 | 3.33 ± 0.02 | 305.91 ± 1.89 | 2.98 ± 0.02 | |
GT (119) | 9977.38 ± 87.89 Bb | 333.57 ± 3.72 | 3.38 ± 0.04 | 298.81 ± 2.71 | 3.01 ± 0.02 | |||
TT (3) | 10,230 ± 421.67 ab | 337.7 ± 17.04 | 3.33 ± 0.17 | 305.44 ± 12.43 | 3.00 ± 0.10 | |||
17.74 | 4.41 | 2.48 | 9.48 | 2.19 | ||||
p value | 0.0002 | 0.1109 | 0.2903 | 0.009 | 0.3343 | |||
Corrected p | 0.0016 | 0.8872 | 2.3224 | 0.072 | 2.6744 | |||
2 | GG (550) | 10,878 ± 59.18 Aa | 389.39 ± 2.66 Aa | 3.58 ± 0.02 | 322.33 ± 1.93 Aa | 2.97 ± 0.02 | ||
GT (82) | 10,222 ± 104.36 Bb | 366.03 ± 4.39 Bb | 3.58 ± 0.04 | 301.48 ± 3.20 Bb | 2.95 ± 0.03 | |||
TT (3) | 10,129 ± 433.12 ab | 361.25 ± 17.55 ab | 3.59 ± 0.17 | 302.88 ± 12.802 ab | 2.97 ± 0.10 | |||
44.84 | 34.64 | 0 | 51.09 | 0.58 | ||||
p value | <0.0001 | <0.0001 | 0.9985 | <0.0001 | 0.7473 | |||
Corrected p | <0.0001 | <0.0001 | 7.988 | <0.0001 | 5.9784 | |||
26:g.17089560G>A | 1 | AA (11) | 10,748 ± 231.33 | 354.55 ± 9.42 | 3.30 ± 0.09 | 321.51 ± 6.87 | 2.98 ± 0.06 | |
AG (153) | 10,285 ± 84.85 | 339.32 ± 3.63 | 3.33 ± 0.03 | 304.86 ± 2.65 | 2.98 ± 0.02 | |||
GG (760) | 10,253 ± 57.75 | 339.19 ± 2.58 | 3.33 ± 0.02 | 304.83 ± 1.88 | 2.99 ± 0.02 | |||
4.77 | 2.9 | 0.15 | 6.47 | 0.22 | ||||
p value | 0.0928 | 0.2354 | 0.9278 | 0.0398 | 0.8947 | |||
Corrected p | 0.7424 | 1.8832 | 7.4224 | 0.3184 | 7.1576 | |||
2 | AA (8) | 10,490 ± 295.13 | 378.43 ± 12.00 | 3.61 ± 0.12 | 319.19 ± 8.7487 | 3.03 ± 0.07 | ||
AG (103) | 10,640 ± 95.67 | 378.74 ± 4.06 | 3.57 ± 0.04 | 315.39 ± 2.9595 | 2.97 ± 0.02 | |||
GG (524) | 10,827 ± 59.57 | 387.96 ± 2.67 | 3.59 ± 0.02 | 320.55 ± 1.944 | 2.96 ± 0.02 | |||
5.16 | 6.55 | 0.28 | 3.73 | 0.97 | ||||
p value | 0.0766 | 0.0387 | 0.8696 | 0.1559 | 0.6172 | |||
Corrected p | 0.6128 | 0.3096 | 6.9568 | 1.2472 | 4.9376 | |||
26:g.17088978A>G | 1 | AA (56) | 10,161 ± 117.76 | 341.96 ± 4.89 | 3.39 ± 0.05 | 305.17 ± 3.57 | 3.01 ± 0.03 | |
AG (306) | 10,250 ± 67.89 | 338.46 ± 2.96 | 3.33 ± 0.03 | 305.17 ± 2.16 | 2.99 ± 0.02 | |||
GG (562) | 10,273 ± 60.16 | 339.35 ± 2.67 | 3.33 ± 0.02 | 304.71 ± 1.95 | 2.98 ± 0.02 | |||
1.02 | 0.65 | 1.71 | 0.09 | 1.44 | ||||
p value | 0.6 | 0.7229 | 0.4256 | 0.9564 | 0.487 | |||
Corrected p | 4.8 | 5.7832 | 3.4048 | 7.6512 | 3.896 | |||
2 | AA (42) | 10,538 ± 139.32 AB | 362.98 ± 5.77 Aa | 3.42 ± 0.06 Aa | 309.2 ± 4.21 Aa | 2.93 ± 0.03 | ||
AG (206) | 10,581 ± 74.38 A | 383.18 ± 3.22 Bb | 3.63 ± 0.03 Bb | 314.45 ± 2.35 Aa | 2.97 ± 0.02 | |||
GG (387) | 10,927 ± 63.06 B | 390.75 ± 2.80 Bb | 3.58 ± 0.03 ab | 323.48 ± 2.04 Bb | 2.97 ± 0.02 | |||
27.58 | 25.8 | 14.13 | 25.1 | 1.74 | ||||
p value | <0.0001 | <0.0001 | 0.0009 | <0.0001 | 0.4186 | |||
Corrected p | <0.0001 | <0.0001 | 0.0072 | <0.0001 | 3.3488 | |||
26:g.17088098G>C | 1 | CC (324) | 10,333 ± 66.66 | 341.06 ± 2.93 | 3.32 ± 0.03 | 305.57 ± 2.13 | 2.97 ± 0.02 | |
CG (426) | 10,215 ± 62.67 | 337.21 ± 2.76 | 3.33 ± 0.03 | 304.24 ± 2.01 | 2.99 ± 0.02 | |||
GG (174) | 10,225 ± 78.95 | 341.27 ± 3.38 | 3.37 ± 0.03 | 305.25 ± 2.46 | 3.00 ± 0.02 | |||
4.57 | 3.89 | 2.16 | 0.73 | 3.49 | ||||
p value | 0.1025 | 0.1436 | 0.3401 | 0.696 | 0.175 | |||
Corrected p | 0.82 | 1.1488 | 2.7208 | 5.568 | 1.4 | |||
2 | CC (233) | 10,970 ± 71.62 Aa | 396.06 ± 3.13 Aa | 3.61 ± 0.03 | 324.66 ± 2.28 Aa | 2.97 ± 0.02 | ||
CG (277) | 10,708 ± 68.62 ABb | 383.33 ± 3.01 Bb | 3.59 ± 0.03 | 317.99 ± 2.19 ABb | 2.97 ± 0.02 | |||
GG (125) | 10,641 ± 87.94 Bb | 374.74 ± 3.74 Bb | 3.52 ± 0.04 | 314.05 ± 2.73 Bb | 2.95 ± 0.02 | |||
17.34 | 34.84 | 6.37 | 16.71 | 1.12 | ||||
p value | 0.0002 | <0.0001 | 0.0423 | 0.0003 | 0.5723 | |||
Corrected p | 0.0016 | <0.0001 | 0.3384 | 0.0024 | 4.5784 | |||
26:g.17086802T>A | 1 | AA (343) | 10,321 ± 65.81 | 341.52 ± 2.89 | 3.33 ± 0.03 | 305.33 ± 2.11 | 2.97 ± 0.02 | |
AT (425) | 10,217 ± 62.63 | 336.33 ± 2.76 | 3.32 ± 0.03 | 304.26 ± 2.01 | 2.99 ± 0.02 | |||
TT (156) | 10,234 ± 81.80 | 343.07 ± 3.49 | 3.38 ± 0.03 | 305.75 ± 2.54 | 3.00 ± 0.02 | |||
3.65 | 8.43 | 4.55 | 0.73 | 3.27 | ||||
p value | 0.1615 | 0.0151 | 0.1034 | 0.6944 | 0.1954 | |||
Corrected p | 1.292 | 0.1208 | 0.8272 | 5.5552 | 1.5632 | |||
2 | AA (247) | 11,016 ± 70.14 Aa | 396.72 ± 3.07 Aa | 3.60 ± 0.03 | 325.69 ± 2.23 Aa | 2.96 ± 0.02 | ||
AT (272) | 10,666 ± 69.12 Bb | 382.36 ± 3.03 Bb | 3.59 ± 0.03 | 316.75 ± 2.20 Bb | 2.97 ± 0.02 | |||
TT (116) | 10,580 ± 90.67 Bb | 372.28 ± 3.85 Bb | 3.52 ± 0.04 | 312.89 ± 2.80 Bb | 2.96 ± 0.02 | |||
31.11 | 45.41 | 5.8 | 26.55 | 0.65 | ||||
p value | <0.0001 | <0.0001 | 0.0559 | <0.0001 | 0.7242 | |||
Corrected p | <0.0001 | <0.0001 | 0.4472 | <0.0001 | 5.7936 | |||
MAT2A | 11:g.49472723G>C | 1 | CC (591) | 10,307 ± 60.23 a | 339.22 ± 2.68 | 3.31 ± 0.02 | 304.92 ± 1.95 | 2.97 ± 0.02 a |
CG (297) | 10,150 ± 67.98 b | 338.26 ± 2.96 | 3.36 ± 0.03 | 304.07 ± 2.15 | 3.01 ± 0.02 b | |||
GG (36) | 10,376 ± 137.72 ab | 348.4 ± 5.68 | 3.39 ± 0.06 | 311.23 ± 4.14 | 3.01 ± 0.03 ab | |||
9.05 | 3.54 | 6.34 | 3.33 | 10.16 | ||||
p value | 0.0111 | 0.1707 | 0.0426 | 0.1899 | 0.0064 | |||
Corrected p | 0.0222 | 0.3414 | 0.0852 | 0.3798 | 0.0128 | |||
2 | CC (410) | 10,829 ± 62.7056 | 387.57 ± 2.79 | 3.58 ± 0.03 | 320.29 ± 2.03 | 2.96 ± 0.02 | ||
CG (201) | 10,707 ± 74.7301 | 384.71 ± 3.24 | 3.59 ± 0.03 | 318.45 ± 2.36 | 2.97 ± 0.02 | |||
GG (24) | 11,006 ± 168.94 | 383.24 ± 6.93 | 3.48 ± 0.07 | 322.02 ± 5.05 | 2.92 ± 0.04 | |||
4.96 | 1.22 | 2.7 | 1.05 | 2.13 | ||||
p value | 0.085 | 0.5426 | 0.2596 | 0.5909 | 0.3455 | |||
Corrected p | 0.17 | 1.0852 | 0.5192 | 1.1818 | 0.691 | |||
11:g.49465032C>T | 1 | CC (454) | 10,313 ± 62.47 a | 340.38 ± 2.76 | 3.33 ± 0.03 | 306.92 ± 2.01 a | 2.99 ± 0.02 | |
CT (351) | 10,167 ± 64.95 b | 336.71 ± 2.85 | 3.34 ± 0.03 | 302.05 ± 2.07 b | 2.98 ± 0.02 | |||
TT (119) | 10,327 ± 86.35 ab | 342.48 ± 3.66 | 3.33 ± 0.04 | 305.58 ± 2.67 ab | 2.97 ± 0.02 | |||
8.8 | 4.57 | 0.14 | 9.83 | 1.07 | ||||
p value | 0.0126 | 0.1022 | 0.9317 | 0.0075 | 0.5869 | |||
Corrected p | 0.0252 | 0.2044 | 1.8634 | 0.015 | 1.1738 | |||
2 | CC (316) | 10,914 ± 66.32 A | 387.23 ± 2.92 ab | 3.55 ± 0.03 | 323.86 ± 2.13 Aa | 2.97 ± 0.02 | ||
CT (235) | 10,763 ± 71.46 AB | 389.58 ± 3.11 a | 3.62 ± 0.03 | 316.58 ± 2.27 Bb | 2.95 ± 0.02 | |||
TT (84) | 10,496 ± 99.15 B | 376.99 ± 4.17 b | 3.60 ± 0.04 | 314.32 ± 3.04 Bb | 3.00 ± 0.03 | |||
18.81 | 9.26 | 6.05 | 18.36 | 5.1 | ||||
p value | <0.0001 | 0.0102 | 0.0495 | 0.0001 | 0.0791 | |||
Corrected p | <0.0001 | 0.0204 | 0.099 | 0.0002 | 0.1582 |
Block | Lactation | Haplotype Combination | Milk Yield (kg) | Fat Yield (kg) | Fat Percentage (%) | Protein Yield (kg) | Protein Percentage (%) |
---|---|---|---|---|---|---|---|
Block1 | 1 | H1H1 (322) | 10,425 ± 67.38 a | 344.75 ± 2.98 | 3.32 ± 0.03 | 308.87 ± 2.17 ab | 2.97 ± 0.02 |
H1H2 (174) | 10,260 ± 77.58 ab | 336.27 ± 3.36 | 3.30 ± 0.032 | 305.59 ± 2.45 ab | 2.99 ± 0.02 | ||
H1H3 (92) | 10,466 ± 96.58 a | 343.07 ± 4.10 | 3.29 ± 0.04 | 311.62 ± 2.99 ab | 2.99 ± 0.03 | ||
H1H4 (68) | 10,422 ± 105.67 ab | 347.38 ± 4.43 | 3.34 ± 0.04 | 313.52 ± 3.23 a | 3.02 ± 0.03 | ||
H1H5 (71) | 10,097 ± 105.9 b | 334.61 ± 4.46 | 3.34 ± 0.04 | 301.61 ± 3.25 b | 3.00 ± 0.03 | ||
15.59 | 14.34 | 2.01 | 14.25 | 4.59 | |||
p value | 0.0039 | 0.0067 | 0.7333 | 0.007 | 0.3327 | ||
Corrected p | 0.0078 | 0.0134 | 1.4666 | 0.014 | 0.6654 | ||
2 | H1H1 (231) | 11,060 ± 71.76 | 398.4 ± 3.15 | 3.60 ± 0.03 | 328.22 ± 2.29 | 2.97 ± 0.02 | |
H1H2 (108) | 10,964 ± 90.72 | 388.92 ± 3.86 | 3.55 ± 0.04 | 327.25 ± 2.81 | 2.98 ± 0.02 | ||
H1H3 (60) | 10,704 ± 115.33 | 381.95 ± 4.83 | 3.59 ± 0.05 | 319.31 ± 3.52 | 2.99 ± 0.03 | ||
H1H4 (44) | 10,447 ± 132.22 | 379.36 ± 5.51 | 3.62 ± 0.05 | 310.99 ± 4.02 | 2.98 ± 0.03 | ||
H1H5 (50) | 10,320 ± 128.01 | 373.52 ± 5.33 | 3.62 ± 0.05 | 307.29 ± 3.89 | 2.98 ± 0.03 | ||
50.87 | 35.23 | 2.6 | 47.84 | 0.53 | |||
p value | <0.0001 | <0.0001 | 0.769 | <0.0001 | 0.9707 | ||
Corrected p | <0.0001 | <0.0001 | 1.538 | <0.0001 | 1.9414 | ||
Block2 | 1 | H1H1 (711) | 10,351 ± 58.33 | 343.72 ± 2.61 | 3.34 ± 0.02 | 307.27 ± 1.90 | 2.97 ± 0.02 |
H1H2 (191) | 10,342 ± 75.13 | 343.81 ± 3.24 | 3.35 ± 0.03 | 306.99 ± 2.36 | 2.98 ± 0.02 | ||
0.03 | 0 | 0.39 | 0.02 | 0.08 | |||
p value | 0.8736 | 0.9716 | 0.5314 | 0.879 | 0.7734 | ||
Corrected p | 1.7472 | 1.9432 | 1.0628 | 1.758 | 1.5468 | ||
2 | H1H1 (489) | 10,804 ± 61.05 Aa | 386.12 ± 2.73 a | 3.5771 ± 0.03 | 321.12 ± 1.99 Aa | 2.97 ± 0.02 | |
H1H2 (133) | 11,083 ± 83.35 Bb | 393.98 ± 3.55 b | 3.5669 ± 0.03388 | 328.79 ± 2.59 Bb | 2.96 ± 0.02 | ||
12.98 | 6.29 | 0.11 | 11.25 | 0.11 | |||
p value | 0.0003 | 0.0125 | 0.7416 | 0.0009 | 0.7456 | ||
Corrected p | 0.0006 | 0.025 | 1.4832 | 0.0018 | 1.4912 |
Gene | SNPs | Allele | Transcription Factor | Relative Score (≥0.80; Jasper) | Predicted Binding Site Sequence (Genomatix) |
---|---|---|---|---|---|
ALDH18A1 | 26:g.17130318C>A | C | HMBOX1 | 0.92 | ACTAGTTTAG |
A | |||||
MAT2A | 11:g.49472723G>C | G | |||
C | MAZ | 0.84 | CGCGGCTCCCC |
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Ye, W.; Xu, L.; Li, Y.; Liu, L.; Ma, Z.; Sun, D.; Han, B. Single Nucleotide Polymorphisms of ALDH18A1 and MAT2A Genes and Their Genetic Associations with Milk Production Traits of Chinese Holstein Cows. Genes 2022, 13, 1437. https://doi.org/10.3390/genes13081437
Ye W, Xu L, Li Y, Liu L, Ma Z, Sun D, Han B. Single Nucleotide Polymorphisms of ALDH18A1 and MAT2A Genes and Their Genetic Associations with Milk Production Traits of Chinese Holstein Cows. Genes. 2022; 13(8):1437. https://doi.org/10.3390/genes13081437
Chicago/Turabian StyleYe, Wen, Lingna Xu, Yanhua Li, Lin Liu, Zhu Ma, Dongxiao Sun, and Bo Han. 2022. "Single Nucleotide Polymorphisms of ALDH18A1 and MAT2A Genes and Their Genetic Associations with Milk Production Traits of Chinese Holstein Cows" Genes 13, no. 8: 1437. https://doi.org/10.3390/genes13081437
APA StyleYe, W., Xu, L., Li, Y., Liu, L., Ma, Z., Sun, D., & Han, B. (2022). Single Nucleotide Polymorphisms of ALDH18A1 and MAT2A Genes and Their Genetic Associations with Milk Production Traits of Chinese Holstein Cows. Genes, 13(8), 1437. https://doi.org/10.3390/genes13081437