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Volume 13
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10.3390/genes13071133
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Article

Identification of Novel Circular RNAs of the Human Protein Arginine Methyltransferase 1 (PRMT1) Gene, Expressed in Breast Cancer Cells

by
Maria Papatsirou
1,
Marios A. Diamantopoulos
1,
Katerina Katsaraki
1,
Dimitris Kletsas
2,
Christos K. Kontos
1,* and
Andreas Scorilas
1
1
Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, Greece
2
Laboratory of Cell Proliferation & Ageing, Institute of Biosciences and Applications, National Centre for Scientific Research “Demokritos”, 15341 Aghia Paraskevi, Greece
*
Author to whom correspondence should be addressed.
Genes 2022, 13(7), 1133; https://doi.org/10.3390/genes13071133
Submission received: 25 May 2022 / Revised: 16 June 2022 / Accepted: 21 June 2022 / Published: 24 June 2022
(This article belongs to the Special Issue Alternative Splicing in Human Physiology and Disease)
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Abstract

Circular RNAs (circRNAs) constitute a type of RNA formed through back-splicing. In breast cancer, circRNAs are implicated in tumor onset and progression. Although histone methylation by PRMT1 is largely involved in breast cancer development and metastasis, the effect of circular transcripts deriving from this gene has not been examined. In this study, total RNA was extracted from four breast cancer cell lines and reversely transcribed using random hexamer primers. Next, first- and second-round PCRs were performed using gene-specific divergent primers. Sanger sequencing followed for the determination of the sequence of each novel PRMT1 circRNA. Lastly, bioinformatics analysis was conducted to predict the functions of the novel circRNAs. In total, nine novel circRNAs were identified, comprising both complete and truncated exons of the PRMT1 gene. Interestingly, we demonstrated that the back-splice junctions consist of novel splice sites of the PRMT1 exons. Moreover, the circRNA expression pattern differed among these four breast cancer cell lines. All the novel circRNAs are predicted to act as miRNA and/or protein sponges, while five circRNAs also possess an open reading frame. In summary, we described the complete sequence of nine novel circRNAs of the PRMT1 gene, comprising distinct back-splice junctions and probably having different molecular properties.
Keywords: circRNAs; PRMT1; sequencing; breast cancer; alternative splicing; back-splice junction; RNA-binding proteins; miRNAs; bioinformatics; RNA modifications circRNAs; PRMT1; sequencing; breast cancer; alternative splicing; back-splice junction; RNA-binding proteins; miRNAs; bioinformatics; RNA modifications

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MDPI and ACS Style

Papatsirou, M.; Diamantopoulos, M.A.; Katsaraki, K.; Kletsas, D.; Kontos, C.K.; Scorilas, A. Identification of Novel Circular RNAs of the Human Protein Arginine Methyltransferase 1 (PRMT1) Gene, Expressed in Breast Cancer Cells. Genes 2022, 13, 1133. https://doi.org/10.3390/genes13071133

AMA Style

Papatsirou M, Diamantopoulos MA, Katsaraki K, Kletsas D, Kontos CK, Scorilas A. Identification of Novel Circular RNAs of the Human Protein Arginine Methyltransferase 1 (PRMT1) Gene, Expressed in Breast Cancer Cells. Genes. 2022; 13(7):1133. https://doi.org/10.3390/genes13071133

Chicago/Turabian Style

Papatsirou, Maria, Marios A. Diamantopoulos, Katerina Katsaraki, Dimitris Kletsas, Christos K. Kontos, and Andreas Scorilas. 2022. "Identification of Novel Circular RNAs of the Human Protein Arginine Methyltransferase 1 (PRMT1) Gene, Expressed in Breast Cancer Cells" Genes 13, no. 7: 1133. https://doi.org/10.3390/genes13071133

APA Style

Papatsirou, M., Diamantopoulos, M. A., Katsaraki, K., Kletsas, D., Kontos, C. K., & Scorilas, A. (2022). Identification of Novel Circular RNAs of the Human Protein Arginine Methyltransferase 1 (PRMT1) Gene, Expressed in Breast Cancer Cells. Genes, 13(7), 1133. https://doi.org/10.3390/genes13071133

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