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Volume 13
Issue 3
10.3390/genes13030475
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Article
Peer-Review Record

Molecular Epidemiology of Mycobacterium tuberculosis Complex Strains in Urban and Slum Settings of Nairobi, Kenya

Genes 2022, 13(3), 475; https://doi.org/10.3390/genes13030475
by Glennah Kerubo 1,2,*, Perpetua Ndungu 3, Yassir Adam Shuaib 1,4, Evans Amukoye 5, Gunturu Revathi 6, Susanne Homolka 1, Samuel Kariuki 7,†, Matthias Merker 1,8,9,† and Stefan Niemann 1,8,*,†
Reviewer 2: Anonymous
Reviewer 3: Anonymous
Genes 2022, 13(3), 475; https://doi.org/10.3390/genes13030475
Submission received: 7 February 2022 / Revised: 2 March 2022 / Accepted: 2 March 2022 / Published: 8 March 2022
(This article belongs to the Special Issue Tuberculosis Genomics and Transcriptomics: From Epidemiological to Evolutionary Aspects)

Round 1

Reviewer 1 Report

This paper presents the results of whole genome sequencing of around 400 mycobacterium tuberculosis isolates from Nairobi, Kenya.

The study is interesting and well designed.

Comments

  1. I could not access the supplementary file describing the setting from where the samples were taken.
  2. A description of the demographics and definition of a slum area and urban area would be helpful
  3. More information on the index cases would be useful - this is mentioned as a limitation by the authors
  4. Was DST carried out for the samples and if so was this compared to the genetic predictions?

Author Response

Please see the attachment

Reviewer 2 Report

TB, and MDR TB in paticular, is still a serious problem in Kenya.
Investigations performed worldwide show that WGS for determining mutations associated with MDR TB gives quick results of MBT resistance to key anti-TB drugs, which allows the doctor to prescribe an adequate chemotherapy regimen to the patient at the very beginning of treatment. However, upon receipt of the MTB culture, the genotypic method
should be further duplicated by the phenotypic drug sensitivity testing
method, based on the results of which, if necessary, the treatment regimen
is adjusted. Here the authors used TB-WGS for drug resistance prediction and epidemiological services in Nairobi. 

  1. The authors did not mention any ethical approvement of the study. Did probands sign informed consent?
  2. Also the description lacks information on the type of chemothrapy the patients received.
  3. At wich interval from the start of chemotherapy the probes for MTB cultures were obtained?

Author Response

Please see the attachment

Author Response File: Author Response.docx

Reviewer 3 Report

Dear Editor,

Thank you for the invitation.

After careful reading, the manuscript is very interesting and there are a few comments for further improvement of the manuscript.

Sincerely,

 

Comments for author File: Comments.pdf

Author Response

Please see the attachment

Author Response File: Author Response.docx

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