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Volume 13
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10.3390/genes13020192
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Article

The SLC27A1 Gene and Its Enriched PPAR Pathway Are Involved in the Regulation of Flavor Compound Hexanal Content in Chinese Native Chickens

by
Yuxi Jin
1,†,
Xiaoya Yuan
2,
Wenjuan Zhao
3,
Hua Li
3,
Guiping Zhao
2,* and
Jianfeng Liu
1,*
1
National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
2
State Key Laboratory of Animal Nutrition, Chinese Academy of Agricultural Science, Beijing 100193, China
3
Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, Foshan University, Foshan 528225, China
*
Authors to whom correspondence should be addressed.
†
Yuxi Jin is the first author.
Genes 2022, 13(2), 192; https://doi.org/10.3390/genes13020192
Submission received: 16 December 2021 / Revised: 17 January 2022 / Accepted: 19 January 2022 / Published: 22 January 2022
(This article belongs to the Special Issue Advances in Poultry Genetics and Genomics)
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Abstract

The role of hexanal in flavor as an indicator of the degree of oxidation of meat products is undeniable. However, the genes and pathways of hexanal formation have not been characterized in detail. In this study, we performed differential gene expression analysis and weighted gene co-expression network analysis (WGCNA) on groups of Tiannong partridge chickens with different relative hexanal content in order to find the genes involved in the formation of hexanal and the specific pathways of hexanal formation. Then we confirmed the relationship of these candidate genes with hexanal using Jingxing Yellow chicken and Wenchang chicken. In this study, WGCNA revealed a module of co-expressed genes that were highly associated with the volatile organic compound hexanal. We also compared transcriptome gene expression data of samples from chicken groups with high and low relative contents of hexanal and identified a total of 651 differentially expressed genes (DEGs). Among them, 356 genes were up regulated, and 295 genes were downregulated. The different biological functions associated with the DEGs, hub genes and hexanal were identified by functional analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations. Among all the hub genes in the significant module identified by WGCNA, more were enriched in the PPAR signaling pathway, the proteasome pathway, etc. Additionally, we found that DEGs and hub genes, including SLC27A1, ACOX3, NR4A1, VEGFA, JUN, EGR1, CACNB1, GADD45A and DUSP1, were co-enriched in the peroxisome proliferator-activated receptor (PPAR) signaling pathway, p53 signaling pathway and mitogen-activated protein kinases (MAPK) signaling pathway, etc. Transcriptome results of the Jingxing Yellow chicken population showed that the SLC27A1 gene was significantly associated with hexanal and enriched in the PPAR pathway. Our study provides a comprehensive insight into the key genes related to hexanal content, and can be further explored by functional and molecular studies.
Keywords: hexanal; RNA-seq; yellow-feathered broiler; WGCNA; SLC27A1 hexanal; RNA-seq; yellow-feathered broiler; WGCNA; SLC27A1

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MDPI and ACS Style

Jin, Y.; Yuan, X.; Zhao, W.; Li, H.; Zhao, G.; Liu, J. The SLC27A1 Gene and Its Enriched PPAR Pathway Are Involved in the Regulation of Flavor Compound Hexanal Content in Chinese Native Chickens. Genes 2022, 13, 192. https://doi.org/10.3390/genes13020192

AMA Style

Jin Y, Yuan X, Zhao W, Li H, Zhao G, Liu J. The SLC27A1 Gene and Its Enriched PPAR Pathway Are Involved in the Regulation of Flavor Compound Hexanal Content in Chinese Native Chickens. Genes. 2022; 13(2):192. https://doi.org/10.3390/genes13020192

Chicago/Turabian Style

Jin, Yuxi, Xiaoya Yuan, Wenjuan Zhao, Hua Li, Guiping Zhao, and Jianfeng Liu. 2022. "The SLC27A1 Gene and Its Enriched PPAR Pathway Are Involved in the Regulation of Flavor Compound Hexanal Content in Chinese Native Chickens" Genes 13, no. 2: 192. https://doi.org/10.3390/genes13020192

APA Style

Jin, Y., Yuan, X., Zhao, W., Li, H., Zhao, G., & Liu, J. (2022). The SLC27A1 Gene and Its Enriched PPAR Pathway Are Involved in the Regulation of Flavor Compound Hexanal Content in Chinese Native Chickens. Genes, 13(2), 192. https://doi.org/10.3390/genes13020192

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