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Article

Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus

1
School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane 4072, Australia
2
CSIRO, Australian Centre for Disease Preparedness, Health, and Biosecurity Business Unit, Geelong 3219, Australia
3
Department of Animal Science, University of California, Davis, CA 95616, USA
4
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
5
Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane 4072, Australia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Jae Yong Han
Genes 2021, 12(6), 901; https://doi.org/10.3390/genes12060901
Received: 11 May 2021 / Revised: 5 June 2021 / Accepted: 8 June 2021 / Published: 10 June 2021
(This article belongs to the Special Issue Avian Viruses and Host Responses)
Highly pathogenic avian influenza viruses (HPAIVs) in gallinaceous poultry are associated with viral infection of the endothelium, the induction of a ‘cytokine storm, and severe disease. In contrast, in Pekin ducks, HPAIVs are rarely endothelial tropic, and a cytokine storm is not observed. To date, understanding these species-dependent differences in pathogenesis has been hampered by the absence of a pure culture of duck and chicken endothelial cells. Here, we use our recently established in vitro cultures of duck and chicken aortic endothelial cells to investigate species-dependent differences in the response of endothelial cells to HPAIV H5N1 infection. We demonstrate that chicken and duck endothelial cells display a different transcriptional response to HPAI H5N1 infection in vitro—with chickens displaying a more pro-inflammatory response to infection. As similar observations were recorded following in vitro stimulation with the viral mimetic polyI:C, these findings were not specific to an HPAIV H5N1 infection. However, similar species-dependent differences in the transcriptional response to polyI:C were not observed in avian fibroblasts. Taken together, these data demonstrate that chicken and duck endothelial cells display a different response to HPAIV H5N1 infection, and this may help account for the species-dependent differences observed in inflammation in vivo. View Full-Text
Keywords: avian influenza; endothelial cells; chicken; duck; cytokine storm; H5N1 avian influenza; endothelial cells; chicken; duck; cytokine storm; H5N1
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MDPI and ACS Style

Tong, Z.W.M.; Karawita, A.C.; Kern, C.; Zhou, H.; Sinclair, J.E.; Yan, L.; Chew, K.Y.; Lowther, S.; Trinidad, L.; Challagulla, A.; Schat, K.A.; Baker, M.L.; Short, K.R. Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus. Genes 2021, 12, 901. https://doi.org/10.3390/genes12060901

AMA Style

Tong ZWM, Karawita AC, Kern C, Zhou H, Sinclair JE, Yan L, Chew KY, Lowther S, Trinidad L, Challagulla A, Schat KA, Baker ML, Short KR. Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus. Genes. 2021; 12(6):901. https://doi.org/10.3390/genes12060901

Chicago/Turabian Style

Tong, Zhen W.M.; Karawita, Anjana C.; Kern, Colin; Zhou, Huaijun; Sinclair, Jane E.; Yan, Limin; Chew, Keng Y.; Lowther, Sue; Trinidad, Lee; Challagulla, Arjun; Schat, Karel A.; Baker, Michelle L.; Short, Kirsty R. 2021. "Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus" Genes 12, no. 6: 901. https://doi.org/10.3390/genes12060901

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