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Article

Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis

1
Graduate School of Environmental Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan
2
Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, Hokkaido 060-0811, Japan
3
Department of Crop Sciences, University of Illinois, Urbana-Champaign, Urbana, IL 61801, USA
4
Global Center for the Research of Food, Land and Water Resources, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Paloma Mas
Genes 2021, 12(2), 288; https://doi.org/10.3390/genes12020288
Received: 7 January 2021 / Revised: 10 February 2021 / Accepted: 15 February 2021 / Published: 19 February 2021
(This article belongs to the Special Issue Molecular Mechanisms of Circadian Clock Function in Plants)
The optimal flowering time for bioenergy crop Miscanthus is essential for environmental adaptability and biomass accumulation. However, little is known about how genes controlling flowering in other grasses contribute to flowering regulation in Miscanthus. Here, we report on the sequence characterization and gene expression of Miscanthus sinensisGhd8, a transcription factor encoding a HAP3/NF-YB DNA-binding domain, which has been identified as a major quantitative trait locus in rice, with pleiotropic effects on grain yield, heading date and plant height. In M. sinensis, we identified two homoeologous loci, MsiGhd8A located on chromosome 13 and MsiGhd8B on chromosome 7, with one on each of this paleo-allotetraploid species’ subgenomes. A total of 46 alleles and 28 predicted protein sequence types were identified in 12 wild-collected accessions. Several variants of MsiGhd8 showed a geographic and latitudinal distribution. Quantitative real-time PCR revealed that MsiGhd8 expressed under both long days and short days, and MsiGhd8B showed a significantly higher expression than MsiGhd8A. The comparison between flowering time and gene expression indicated that MsiGhd8B affected flowering time in response to day length for some accessions. This study provides insight into the conserved function of Ghd8 in the Poaceae, and is an important initial step in elucidating the flowering regulatory network of Miscanthus. View Full-Text
Keywords: Miscanthus sinensis; Ghd8; gene expression; flowering time; sequence diversity; geographic distribution Miscanthus sinensis; Ghd8; gene expression; flowering time; sequence diversity; geographic distribution
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MDPI and ACS Style

Guo, Z.; Xu, M.; Nagano, H.; Clark, L.V.; Sacks, E.J.; Yamada, T. Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis. Genes 2021, 12, 288. https://doi.org/10.3390/genes12020288

AMA Style

Guo Z, Xu M, Nagano H, Clark LV, Sacks EJ, Yamada T. Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis. Genes. 2021; 12(2):288. https://doi.org/10.3390/genes12020288

Chicago/Turabian Style

Guo, Zhihui, Meilan Xu, Hironori Nagano, Lindsay V. Clark, Erik J. Sacks, and Toshihiko Yamada. 2021. "Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis" Genes 12, no. 2: 288. https://doi.org/10.3390/genes12020288

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