MiRNA-Regulated Pathways for Hypertrophic Cardiomyopathy: Network-Based Approach to Insight into Pathogenesis
Abstract
:1. Introduction
2. Methods
2.1. Workflow
2.2. Tools and Databases
2.3. Availability of Data and Materials
3. Results
3.1. Identification of Key Target Genes and Analysis of Their Overrepresentation in Reactome Gene Sets
3.2. Greedy Search for Pathways Shared by Studied miRNAs
3.3. Analysis of miRNA Interactions with Key Targets Included in TGFβ-Mediated SMAD Signaling Pathways
3.4. Analysis of the Consistency of the Algorithm Used to Select Key Target Genes
4. Discussion
5. Limitations
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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miRNA | Number of Targets | |||
---|---|---|---|---|
Total, N | Expressed in Heart Tissue, N (% of Total) | Included in LCC *, N (% of Total) | Identified as Key Genes of LCC *, N (% of Total) | |
hsa-miR-1-3p | 921 | 504 (55) | 181 (20) | 70 (8) |
hsa-miR-19b-3p | 714 | 425 (60) | 108 (15) | 13 (2) |
hsa-miR-21-5p | 612 | 353 (58) | 99 (16) | 26 (4) |
hsa-miR-29a-3p | 265 | 138 (52) | 28 (11) | 7 (3) |
hsa-miR-93-5p | 1220 | 718 (59) | 284 (23) | 56 (5) |
hsa-miR-133a-3p | 131 | 65 (50) | 7 (5) | 5 (4) |
hsa-miR-155-5p | 904 | 530 (59) | 214 (24) | 29 (3) |
hsa-miR-199a-3p | 114 | 66 (58) | 11 (10) | 6 (5) |
hsa-miR-221-3p | 368 | 207 (56) | 57 (15) | 10 (3) |
hsa-miR-222-3p | 394 | 242 (61) | 42 (11) | 10 (3) |
hsa-miR-451a | 31 | 16 (52) | 6 (19) | 5 (16) |
hsa-miR-497-5p | 461 | 254 (55) | 78 (17) | 13 (3) |
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Osmak, G.; Baulina, N.; Kiselev, I.; Favorova, O. MiRNA-Regulated Pathways for Hypertrophic Cardiomyopathy: Network-Based Approach to Insight into Pathogenesis. Genes 2021, 12, 2016. https://doi.org/10.3390/genes12122016
Osmak G, Baulina N, Kiselev I, Favorova O. MiRNA-Regulated Pathways for Hypertrophic Cardiomyopathy: Network-Based Approach to Insight into Pathogenesis. Genes. 2021; 12(12):2016. https://doi.org/10.3390/genes12122016
Chicago/Turabian StyleOsmak, German, Natalia Baulina, Ivan Kiselev, and Olga Favorova. 2021. "MiRNA-Regulated Pathways for Hypertrophic Cardiomyopathy: Network-Based Approach to Insight into Pathogenesis" Genes 12, no. 12: 2016. https://doi.org/10.3390/genes12122016