Cell-Cycle–Dependent Chromatin Dynamics at Replication Origins
Abstract
:1. Introduction
2. Materials and Methods
2.1. Yeast Culture and Cell-Cycle Time Courses
2.2. Chromatin Digestion with MNase and Sequencing Library Preparation
2.3. Flow Cytometry
2.4. Sequencing Data Processing and Analysis
2.5. Quantification of Nucleosome Occupancy
2.6. Quantification of Small Fragment Occupancy
2.7. Quantification of Nucleosome Disorganization by Shannon Entropy
3. Results
3.1. Chromatin Occupancy Profiling of Replication Origins Throughout the Cell Cycle
3.2. Cell-Cycle–Dependent Changes in Replication Initiation Factor Occupancy at Replication Origins
3.3. Cell-Cycle–Regulated Nucleosome Occupancy Dynamics Around Replication Origins
3.4. Replication-Coupled Nucleosome Disorganization
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, Y.; Hartemink, A.J.; MacAlpine, D.M. Cell-Cycle–Dependent Chromatin Dynamics at Replication Origins. Genes 2021, 12, 1998. https://doi.org/10.3390/genes12121998
Li Y, Hartemink AJ, MacAlpine DM. Cell-Cycle–Dependent Chromatin Dynamics at Replication Origins. Genes. 2021; 12(12):1998. https://doi.org/10.3390/genes12121998
Chicago/Turabian StyleLi, Yulong, Alexander J. Hartemink, and David M. MacAlpine. 2021. "Cell-Cycle–Dependent Chromatin Dynamics at Replication Origins" Genes 12, no. 12: 1998. https://doi.org/10.3390/genes12121998