Comprehensive Genome-Wide Identification and Transcript Profiling of GABA Pathway Gene Family in Apple (Malus domestica)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification and Classification of the Apple GABA Pathway Genes
2.2. Phylogenetic Analysis of the GABA Pathway Proteins
2.3. Chromosomal Distribution and Synteny Analysis
2.4. Sequences Analysis
2.5. GABA Pathway Genes Expression Profiles
3. Results
3.1. Genome-Wide Identification and Characterization of GABA Pathway Gene Family Members in Apple
3.2. Chromosome Distribution and Duplication of Apple GABA Shunt Gene Family
3.3. Evolutionary Relationships of GABA Shunt Genes between Apple and Arabidopsis
3.4. Gene Structure Analysis and Conserved Motif Identification in Apple GABA Pathway Genes
3.5. Expression Profiles of GABA Pathway Genes in Different Tissues Development, Biological and Abiotic Stress
3.6. Cis-Acting Elements in the Promoter of the GABA Pathway Genes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Gene Name Abbreviation | Locus | Chromosome | Start Position | End Position | Strand | Length (aa) | pI | MW (kD) | Subcellular Localization Predicted | Potential Functions |
---|---|---|---|---|---|---|---|---|---|---|---|
Putative glutamate decarboxylase | MdGAD1 | MD06G1235500 | Chr06 | 36665970 | 36669767 | − | 505 | 5.49 | 56.50 | cyto | GABA Biosynthesis |
MdGAD2 | MD09G1276300 | Chr09 | 35359458 | 35366901 | − | 501 | 5.62 | 56.93 | cyto | ||
MdGAD3 | MD09G1277000 | Chr09 | 35381876 | 35388878 | − | 501 | 5.71 | 56.85 | cyto | ||
MdGAD4 | MD14G1242700 | Chr14 | 32108140 | 32110957 | − | 333 | 4.95 | 37.68 | cyto | ||
MdGAD5 | MD16G1010800 | Chr16 | 826461 | 829289 | + | 510 | 5.65 | 56.95 | chlo | ||
Putative GABA transaminase | MdGABA-T1 | MD09G1139200 | Chr09 | 10792690 | 10798780 | − | 521 | 6.95 | 57.59 | chlo | GABA Catabolism |
MdGABA-T2 | MD17G1128700 | Chr17 | 11307951 | 11313609 | − | 514 | 6.95 | 56.69 | chlo | ||
Putative GABA transporters | MdGAT1 | MD02G1244800 | Chr02 | 29475795 | 29480278 | − | 508 | 9.07 | 56.22 | plas | GABA Transport |
MdGAT2 | MD03G1133300 | Chr03 | 13280438 | 13300479 | − | 510 | 9.28 | 56.40 | plas | ||
MdGAT3 | MD06G1103100 | Chr06 | 23991060 | 23995998 | + | 452 | 8.91 | 49.64 | cyto | ||
MdGAT4 | MD07G1072100 | Chr07 | 6824513 | 6827647 | + | 460 | 8.75 | 50.35 | plas | ||
MdGAT5 | MD07G1072200 | Chr07 | 6832373 | 6833039 | + | 174 | 4.79 | 19.42 | cyto | ||
MdGAT6 | MD07G1072300 | Chr07 | 6833186 | 6834458 | + | 299 | 9.22 | 32.55 | chlo | ||
MdGAT7 | MD10G1191600 | Chr10 | 28831972 | 28834442 | + | 457 | 8.97 | 50.79 | plas | ||
MdGAT8 | MD11G1155800 | Chr11 | 14931465 | 14932148 | − | 177 | 9.88 | 19.42 | vacu | ||
MdGAT9 | MD11G1156000 | Chr11 | 14949839 | 14952668 | − | 202 | 7.69 | 22.50 | plas | ||
MdGAT10 | MD14G1122000 | Chr14 | 19672859 | 19676484 | + | 452 | 8.97 | 49.81 | plas | ||
Putative GABA receptors | MdALMT1 | MD00G1017600 | Chr00 | 2696703 | 2698931 | − | 472 | 8.21 | 51.81 | plas | GABA Receptors |
MdALMT2 | MD00G1049200 | Chr00 | 9253069 | 9261444 | + | 485 | 9.28 | 53.69 | plas | ||
MdALMT4 | MD03G1155200 | Chr03 | 17835181 | 17837669 | + | 476 | 8.73 | 52.20 | plas | ||
MdALMT5 | MD03G1155400 | Chr03 | 18018465 | 18020999 | + | 484 | 8.83 | 53.34 | plas | ||
MdALMT14 | MD11G1173000 | Chr11 | 19922876 | 19925870 | + | 485 | 9.39 | 53.75 | plas | ||
MdALMT17 | MD12G1040500 | Chr12 | 4405915 | 4408221 | − | 493 | 8.30 | 53.72 | plas | ||
MdALMT20 | MD14G1039500 | Chr14 | 3629641 | 3631853 | − | 494 | 8.84 | 53.85 | plas |
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Zheng, Q.; Su, S.; Wang, Z.; Wang, Y.; Xu, X. Comprehensive Genome-Wide Identification and Transcript Profiling of GABA Pathway Gene Family in Apple (Malus domestica). Genes 2021, 12, 1973. https://doi.org/10.3390/genes12121973
Zheng Q, Su S, Wang Z, Wang Y, Xu X. Comprehensive Genome-Wide Identification and Transcript Profiling of GABA Pathway Gene Family in Apple (Malus domestica). Genes. 2021; 12(12):1973. https://doi.org/10.3390/genes12121973
Chicago/Turabian StyleZheng, Qingbo, Shenghui Su, Zhe Wang, Yongzhang Wang, and Xiaozhao Xu. 2021. "Comprehensive Genome-Wide Identification and Transcript Profiling of GABA Pathway Gene Family in Apple (Malus domestica)" Genes 12, no. 12: 1973. https://doi.org/10.3390/genes12121973