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Article

The Persistence of Staphylococcus aureus in Pressure Ulcers: A Colonising Role

1
Virulence Bactérienne et Infections Chroniques, INSERM U1047, Université de Montpellier, Service de Microbiologie et Hygiène Hospitalière, CHU Nîmes, 30908 Nîmes, France
2
IRD, Microbes, Evolution, Phylogeny and Infection (MEPHI), Aix-Marseille-Université, IHU Méditerranée Infection, 13005 Marseille, France
3
Centre Mutualiste Neurologique Propara, 34090 Montpellier, France
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Virulence Bactérienne et Infections Chroniques, INSERM U1047, Université de Montpellier, 30908 Nîmes, France
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Virulence Bactérienne et Infections Chroniques, INSERM U1047, Université de Montpellier, Service de Maladies Infectieuses et Tropicales, CHU Nîmes, 30908 Nîmes, France
*
Author to whom correspondence should be addressed.
Academic Editor: Patrice Francois
Genes 2021, 12(12), 1883; https://doi.org/10.3390/genes12121883 (registering DOI)
Received: 24 October 2021 / Revised: 19 November 2021 / Accepted: 22 November 2021 / Published: 25 November 2021
(This article belongs to the Special Issue Genetics, Genomics and Pathogenesis of Staphylococcus aureus)
Decubitus pressure ulcers (PU) are a major complication of immobilised patients. Staphylococcus aureus is one of the most frequently detected microorganisms in PU samples; however, its persistence and role in the evolution of these wounds is unknown. In this study, we analysed S. aureus strains isolated from PU biopsies at inclusion and day 28. Eleven S. aureus (21.1%) were detected in 52 patients at inclusion. Only six PUs (11.5%) continued to harbour this bacterium at day 28. Using a whole genome sequencing approach (Miseq®, Illumina), we confirmed that these six S. aureus samples isolated at D28 were the same strain as that isolated at inclusion, with less than 83 bp difference. Phenotypical studies evaluating the growth profiles (Infinite M Mano, Tecan®) and biofilm formation (Biofilm Ring Test®) did not detect any significant difference in the fitness of the pairs of S. aureus. However, using the Caenorhabditis elegans killing assay, a clear decrease of virulence was observed between strains isolated at D28 compared with those isolated at inclusion, regardless of the clinical evolution of the PU. Moreover, all strains at inclusion were less virulent than a control S. aureus strain, i.e., NSA739. An analysis of polymicrobial communities of PU (by metabarcoding approach), in which S. aureus persisted, demonstrated no impact of Staphylococcus genus on PU evolution. Our study suggested that S. aureus presented a colonising profile on PU with no influence on wound evolution. View Full-Text
Keywords: chronic wound; colonisation; decubitus pressure ulcers; persistence; Staphylococcus aureus chronic wound; colonisation; decubitus pressure ulcers; persistence; Staphylococcus aureus
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MDPI and ACS Style

Fayolle, M.; Morsli, M.; Gelis, A.; Chateauraynaud, M.; Yahiaoui-Martinez, A.; Sotto, A.; Lavigne, J.-P.; Dunyach-Remy, C. The Persistence of Staphylococcus aureus in Pressure Ulcers: A Colonising Role. Genes 2021, 12, 1883. https://doi.org/10.3390/genes12121883

AMA Style

Fayolle M, Morsli M, Gelis A, Chateauraynaud M, Yahiaoui-Martinez A, Sotto A, Lavigne J-P, Dunyach-Remy C. The Persistence of Staphylococcus aureus in Pressure Ulcers: A Colonising Role. Genes. 2021; 12(12):1883. https://doi.org/10.3390/genes12121883

Chicago/Turabian Style

Fayolle, Martin, Madjid Morsli, Anthony Gelis, Marion Chateauraynaud, Alex Yahiaoui-Martinez, Albert Sotto, Jean-Philippe Lavigne, and Catherine Dunyach-Remy. 2021. "The Persistence of Staphylococcus aureus in Pressure Ulcers: A Colonising Role" Genes 12, no. 12: 1883. https://doi.org/10.3390/genes12121883

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