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The BrGI Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage

1
Department of Agricultural Biotechnology, National Institute of Agricultural Science, Rural Development Administration, Jeonju 54874, Korea
2
Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Korea
3
Department of Horticulture, College of Agriculture & Life Sciences, Jeonbuk National University, Jeonju 54896, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Paola Vittorioso
Genes 2021, 12(11), 1664; https://doi.org/10.3390/genes12111664 (registering DOI)
Received: 20 September 2021 / Revised: 20 October 2021 / Accepted: 21 October 2021 / Published: 22 October 2021
(This article belongs to the Section Plant Genetics and Genomics)
Circadian clocks integrate environmental cues with endogenous signals to coordinate physiological outputs. Clock genes in plants are involved in many physiological and developmental processes, such as photosynthesis, stomata opening, stem elongation, light signaling, and floral induction. Many Brassicaceae family plants, including Chinese cabbage (Brassica rapa ssp. pekinensis), produce a unique glucosinolate (GSL) secondary metabolite, which enhances plant protection, facilitates the design of functional foods, and has potential medical applications (e.g., as antidiabetic and anticancer agents). The levels of GSLs change diurnally, suggesting a connection to the circadian clock system. We investigated whether circadian clock genes affect the biosynthesis of GSLs in Brassica rapa using RNAi-mediated suppressed transgenic Brassica rapa GIGENTEA homolog (BrGI knockdown; hereafter GK1) Chinese cabbage. GIGANTEA plays an important role in the plant circadian clock system and is related to various developmental and metabolic processes. Using a validated GK1 transgenic line, we performed RNA sequencing and high-performance liquid chromatography analyses. The transcript levels of many GSL pathway genes were significantly altered in GK1 transgenic plants. In addition, GSL contents were substantially reduced in GK1 transgenic plants. We report that the BrGI circadian clock gene is required for the biosynthesis of GSLs in Chinese cabbage plants. View Full-Text
Keywords: Chinese cabbage; circadian clock; glucosinolate; metabolite; RNAi Chinese cabbage; circadian clock; glucosinolate; metabolite; RNAi
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MDPI and ACS Style

Kim, N.S.; Kim, S.J.; Jo, J.S.; Lee, J.G.; Lee, S.I.; Kim, D.H.; Kim, J.A. The BrGI Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage. Genes 2021, 12, 1664. https://doi.org/10.3390/genes12111664

AMA Style

Kim NS, Kim SJ, Jo JS, Lee JG, Lee SI, Kim DH, Kim JA. The BrGI Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage. Genes. 2021; 12(11):1664. https://doi.org/10.3390/genes12111664

Chicago/Turabian Style

Kim, Nan S., Su J. Kim, Jung S. Jo, Jun G. Lee, Soo I. Lee, Dong H. Kim, and Jin A. Kim 2021. "The BrGI Circadian Clock Gene Is Involved in the Regulation of Glucosinolates in Chinese Cabbage" Genes 12, no. 11: 1664. https://doi.org/10.3390/genes12111664

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