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Article

Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection

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Department of Agronomy Food Natural Resources Animals Environment, Campus of Agripolis, University of Padova, 35020 Legnaro, Italy
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Gruppo Padana Ortofloricoltura S.S., Via Olimpia 41, 31038 Treviso, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Yong-Gu Cho and Kwon-Kyoo Kang
Genes 2021, 12(11), 1656; https://doi.org/10.3390/genes12111656
Received: 13 September 2021 / Revised: 16 October 2021 / Accepted: 18 October 2021 / Published: 21 October 2021
(This article belongs to the Special Issue Genetic Research and Plant Breeding)
Lavender species are widely distributed in their wild forms around the Mediterranean Basin and they are also cultivated worldwide as improved and registered clonal varieties. The economic interest of the species belonging to the Lavandula genus is determined by their use as ornamental plants and important source of essential oils that are destinated to the production of cosmetics, pharmaceuticals and foodstuffs. Because of the increasing number of cases of illegal commercialization of selected varieties, the protection of plant breeders’ rights has become of main relevance for the recognition of breeding companies’ royalties. With this aim, genomic tools based on molecular markers have been demonstrated to be very reliable and transferable among laboratories, and also much more informative than morphological descriptors. With the rising of the next-generation sequencing (NGS) technologies, several genotyping-by-sequencing approaches are now available. This study deals with a deep characterization of 15 varietal clones, belonging to two distinct Lavandula species, by means of restriction-site associated DNA sequencing (RAD-Seq). We demonstrated that this technology screens single nucleotide variants that enable to assess the genetic identity of individual accessions, to reconstruct genetic relationships among related breeding lines, to group them into genetically distinguishable main subclusters, and to assign their molecular lineages to distinct ancestors. Moreover, a number of polymorphic sites were identified within genes putatively involved in biosynthetic pathways related to both tissue pigmentation and terpene production, useful for breeding and/or protecting newly registered varieties. Overall, the results highlighted the presence of pure ancestries and interspecific hybrids for the analyzed Lavandula species, and demonstrated that RAD-Seq analysis is very informative and highly reliable for characterizing Lavandula clones and managing plant variety protection. View Full-Text
Keywords: Lavandula; NGS; genotyping by RAD sequencing; flavonoids; terpenes; chloroplast DNA barcoding; ancestry reconstruction; interspecific crosses; plant breeder’s rights Lavandula; NGS; genotyping by RAD sequencing; flavonoids; terpenes; chloroplast DNA barcoding; ancestry reconstruction; interspecific crosses; plant breeder’s rights
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MDPI and ACS Style

Scariolo, F.; Palumbo, F.; Vannozzi, A.; Sacilotto, G.B.; Gazzola, M.; Barcaccia, G. Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection. Genes 2021, 12, 1656. https://doi.org/10.3390/genes12111656

AMA Style

Scariolo F, Palumbo F, Vannozzi A, Sacilotto GB, Gazzola M, Barcaccia G. Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection. Genes. 2021; 12(11):1656. https://doi.org/10.3390/genes12111656

Chicago/Turabian Style

Scariolo, Francesco, Fabio Palumbo, Alessandro Vannozzi, Gio B. Sacilotto, Marco Gazzola, and Gianni Barcaccia. 2021. "Genotyping Analysis by RAD-Seq Reads Is Useful to Assess the Genetic Identity and Relationships of Breeding Lines in Lavender Species Aimed at Managing Plant Variety Protection" Genes 12, no. 11: 1656. https://doi.org/10.3390/genes12111656

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