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Volume 12
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10.3390/genes12101640
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Article

Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes

by
Zhipeng Li
1,*,†,
Xinhui Song
1,†,
Shan Yin
2,
Jiageng Yan
1,
Peiru Lv
2,
Huiquan Shan
1,
Kuiqing Cui
1,
Hongbo Liu
2,* and
Qingyou Liu
1
1
Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Univesity, Nanning 530005, China
2
Henan Chuangyuan Biotechnology Co. Ltd.; Zhengzhou 451100, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to the manuscript.
Academic Editors: Kun Zhang and Xueming Zhao
Genes 2021, 12(10), 1640; https://doi.org/10.3390/genes12101640
Received: 23 September 2021 / Revised: 13 October 2021 / Accepted: 16 October 2021 / Published: 19 October 2021
(This article belongs to the Special Issue Early Embryonic Development of Animals)
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Abstract

Donkeys are an important domesticated animal, providing labor, meat, milk, and medicinal materials for humans. However, the donkey population is continuously declining and even at risk of extinction. The application of modern animal production technology, such as oocyte in vitro maturation, is a promising method to improve the donkey population. In this study, we explore the gene expression patterns of donkey germinal vesicle (GV) and in vitro matured metaphase II (MII) oocytes using single cell RNA-seq of the candidate genes along with the regulatory mechanisms that affect donkey oocyte maturation. We identified a total of 24,164 oocyte genes of which 9073 were significant differentially expressed in the GV and MII oocytes. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that these genes were associated with the meiotic cell cycle, mitochondrion activity, and N-glycan biosynthesis, which might be the key genes and regulatory mechanisms affecting the maturation of donkey oocytes. Our study provides considerable understanding regarding the maturation of donkey oocytes and serves as a theoretical basis for improving the development of donkey oocytes, which could ultimately benefit the expansion of the donkey population and conservation of biodiversity and genetic resources. View Full-Text
Keywords: single-cell RNA-seq; donkey; oocytes; in vitro maturation; gene expression single-cell RNA-seq; donkey; oocytes; in vitro maturation; gene expression
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MDPI and ACS Style

Li, Z.; Song, X.; Yin, S.; Yan, J.; Lv, P.; Shan, H.; Cui, K.; Liu, H.; Liu, Q. Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes. Genes 2021, 12, 1640. https://doi.org/10.3390/genes12101640

AMA Style

Li Z, Song X, Yin S, Yan J, Lv P, Shan H, Cui K, Liu H, Liu Q. Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes. Genes. 2021; 12(10):1640. https://doi.org/10.3390/genes12101640

Chicago/Turabian Style

Li, Zhipeng, Xinhui Song, Shan Yin, Jiageng Yan, Peiru Lv, Huiquan Shan, Kuiqing Cui, Hongbo Liu, and Qingyou Liu. 2021. "Single-Cell RNA-Seq Revealed the Gene Expression Pattern during the In Vitro Maturation of Donkey Oocytes" Genes 12, no. 10: 1640. https://doi.org/10.3390/genes12101640

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