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Article

Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation

1
Department of Biology, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA 19104, USA
2
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
3
Veterinary Biosciences, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville 3010, Australia
4
Computational Biology Group, Max-Planck-Institute of Biochemistry, 82152 Martinsried, Germany
*
Author to whom correspondence should be addressed.
Genes 2021, 12(1), 58; https://doi.org/10.3390/genes12010058
Received: 21 November 2020 / Revised: 28 December 2020 / Accepted: 29 December 2020 / Published: 31 December 2020
(This article belongs to the Special Issue Molecular Biology of Extremophiles)
Motility regulation plays a key role in prokaryotic responses to environmental stimuli. Here, we used a motility screen and selection to isolate hypermotile Haloferax volcanii mutants from a transposon insertion library. Whole genome sequencing revealed that hypermotile mutants were predominantly affected in two genes that encode HVO_1357 and HVO_2248. Alterations of these genes comprised not only transposon insertions but also secondary genome alterations. HVO_1357 contains a domain that was previously identified in the regulation of bacteriorhodopsin transcription, as well as other domains frequently found in two-component regulatory systems. The genes adjacent to hvo_1357 encode a sensor box histidine kinase and a response regulator, key players of a two-component regulatory system. None of the homologues of HVO_2248 have been characterized, nor does it contain any of the assigned InterPro domains. However, in a significant number of Haloferax species, the adjacent gene codes for a chemotaxis receptor/transducer. Our results provide a foundation for characterizing the root causes underlying Hfx. volcanii hypermotility. View Full-Text
Keywords: Haloferax volcanii; archaea; swimming motility; archaella; chemotaxis; hypermotility selection; transposon mutagenesis; two-component regulatory system; extremophiles Haloferax volcanii; archaea; swimming motility; archaella; chemotaxis; hypermotility selection; transposon mutagenesis; two-component regulatory system; extremophiles
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MDPI and ACS Style

Collins, M.; Afolayan, S.; Igiraneza, A.B.; Schiller, H.; Krespan, E.; Beiting, D.P.; Dyall-Smith, M.; Pfeiffer, F.; Pohlschroder, M. Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation. Genes 2021, 12, 58. https://doi.org/10.3390/genes12010058

AMA Style

Collins M, Afolayan S, Igiraneza AB, Schiller H, Krespan E, Beiting DP, Dyall-Smith M, Pfeiffer F, Pohlschroder M. Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation. Genes. 2021; 12(1):58. https://doi.org/10.3390/genes12010058

Chicago/Turabian Style

Collins, Michiyah, Simisola Afolayan, Aime B. Igiraneza, Heather Schiller, Elise Krespan, Daniel P. Beiting, Mike Dyall-Smith, Friedhelm Pfeiffer, and Mechthild Pohlschroder. 2021. "Mutations Affecting HVO_1357 or HVO_2248 Cause Hypermotility in Haloferax volcanii, Suggesting Roles in Motility Regulation" Genes 12, no. 1: 58. https://doi.org/10.3390/genes12010058

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