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Article

Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells

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Department of Zoology, Genetics and Physical Anthropology, Universidade de Santiago de Compostela, Campus de Lugo, 27002 Lugo, Spain
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Genomic Medicine Group, Center for Research in Molecular Medicine and Chronic Diseases (CiMUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain
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Liquid Biopsy Analysis Unit, Oncomet, Health Research Institute of Santiago de Compostela (IDIS), 15706 Santiago de Compostela, Spain
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Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), 29029 Madrid, Spain
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Translational Laboratory, Medical Oncology Department, Complexo Hospitalario Universitario de Santiago de Compostela/SERGAS, 15706 Santiago de Compostela, Spain
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Preclinical Animal Models Group, Health Research Institute of Santiago de Compostela (IDIS), 15706 Santiago de Compostela, Spain
*
Authors to whom correspondence should be addressed.
Genes 2021, 12(1), 113; https://doi.org/10.3390/genes12010113
Received: 26 November 2020 / Revised: 9 January 2021 / Accepted: 13 January 2021 / Published: 19 January 2021
(This article belongs to the Special Issue Zebrafish Animal Models)
Published studies show that most of the human cancer xenograft studies in zebrafish embryos have used incubation temperatures in the range of 32–34 °C for 3–6 days post-injection, trying to find a compromise temperature between the zebrafish embryos (28 °C) and the human injected cells (37 °C). While this experimental setup is widely used, a question remains: is possible to overcome the drawbacks caused by a suboptimal temperature for the injected cells? To clarify the effect of temperature and injected cells on the host, in this study, we analyzed the development and health of the last in response to different temperatures in the presence or absence of injected human cancer cells. Comparing different incubation temperatures (28, 34 and 36 °C), we determined morphological abnormalities and developmental effects in injected and non-injected embryos at different time points. Besides this, the expression of selected genes was determined by qPCR to determine temperature affected metabolic processes in the embryos. The results indicate that an incubation temperature of 36 °C during a period of 48 h is suitable for xenotransplantation without morphological or metabolic changes that could be affecting the host or the injected cells, allowing them to proliferate near their optimal temperature. View Full-Text
Keywords: zebrafish; development; xenotransplantation; incubation-temperature; gene-expression zebrafish; development; xenotransplantation; incubation-temperature; gene-expression
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MDPI and ACS Style

Cabezas-Sainz, P.; Coppel, C.; Pensado-López, A.; Fernandez, P.; Muinelo-Romay, L.; López-López, R.; Rubiolo, J.A.; Sánchez, L. Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells. Genes 2021, 12, 113. https://doi.org/10.3390/genes12010113

AMA Style

Cabezas-Sainz P, Coppel C, Pensado-López A, Fernandez P, Muinelo-Romay L, López-López R, Rubiolo JA, Sánchez L. Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells. Genes. 2021; 12(1):113. https://doi.org/10.3390/genes12010113

Chicago/Turabian Style

Cabezas-Sainz, Pablo, Carlos Coppel, Alba Pensado-López, Pedro Fernandez, Laura Muinelo-Romay, Rafael López-López, Juan A. Rubiolo, and Laura Sánchez. 2021. "Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells" Genes 12, no. 1: 113. https://doi.org/10.3390/genes12010113

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