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Open AccessArticle

Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence

1
International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Hyderabad 502324, India
2
Department of Genetics, Faculty of Sciences, Osmania University, Hyderabad, Telangana 500007, India
3
International Crop Research Institute for the Semi-Arid Tropics, Niamey BP12404, Niger
4
Department of Biotechnology, Vignan’s Foundation for Science, Technology & Research, Vadlamudi, Guntur, Andhra Pradesh 522213, India
*
Author to whom correspondence should be addressed.
Genes 2020, 11(9), 1026; https://doi.org/10.3390/genes11091026
Received: 14 July 2020 / Revised: 17 August 2020 / Accepted: 20 August 2020 / Published: 1 September 2020
(This article belongs to the Special Issue Genetic Improvement of Cereals and Grain Legumes)
This study was conducted to dissect the genetic basis and to explore the candidate genes underlying one of the important genomic regions on an SBI-10 long arm (L), governing the complex stay-green trait contributing to post-flowering drought-tolerance in sorghum. A fine-mapping population was developed from an introgression line cross—RSG04008-6 (stay-green) × J2614-11 (moderately senescent). The fine-mapping population with 1894 F2 was genotyped with eight SSRs and a set of 152 recombinants was identified, advanced to the F4 generation, field evaluated with three replications over 2 seasons, and genotyped with the GBS approach. A high-resolution linkage map was developed for SBI-10L using 260 genotyping by sequencing—Single Nucleotide Polymorphism (GBS–SNPs). Using the best linear unpredicted means (BLUPs) of the percent green leaf area (%GL) traits and the GBS-based SNPs, we identified seven quantitative trait loci (QTL) clusters and single gene, mostly involved in drought-tolerance, for each QTL cluster, viz., AP2/ERF transcription factor family (Sobic.010G202700), NBS-LRR protein (Sobic.010G205600), ankyrin-repeat protein (Sobic.010G205800), senescence-associated protein (Sobic.010G270300), WD40 (Sobic.010G205900), CPK1 adapter protein (Sobic.010G264400), LEA2 protein (Sobic.010G259200) and an expressed protein (Sobic.010G201100). The target genomic region was thus delimited from 15 Mb to 8 genes co-localized with QTL clusters, and validated using quantitative real-time (qRT)–PCR. View Full-Text
Keywords: sorghum chromosome 10 long arm (SBI-10L), fine-mapping; stay-green; candidate genes; percent green leaf area; delayed senescence sorghum chromosome 10 long arm (SBI-10L), fine-mapping; stay-green; candidate genes; percent green leaf area; delayed senescence
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    Doi: doi.org/10.21421/D2/TO4JWO , doi.org/10.21421/D2/45YOFR
    Link: https://doi.org/10.21421/D2/TO4JWO , https://doi.org/10.21421/D2/45YOFR
    Description: The datasets generated for this study can be found in the repository Dataverse: the DOI link for the deposited SNP data in the Dataverse repository: Caption: Fine mapping for shoot fly resistance and stay-green QTL's in Sorghum https://doi.org/10.21421/D2/TO4JWO and SSR data Caption: Evaluation of QTLs for Shoot Fly (Atherigona soccata) Resistance using 1894 F2 population based on SSR marker data DOI link: https://doi.org/10.21421/D2/45YOFR.
MDPI and ACS Style

Kiranmayee, K.N.S.U.; Hash, C.T.; Sivasubramani, S.; Ramu, P.; Amindala, B.P.; Rathore, A.; Kishor, P.B.K.; Gupta, R.; Deshpande, S.P. Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence. Genes 2020, 11, 1026. https://doi.org/10.3390/genes11091026

AMA Style

Kiranmayee KNSU, Hash CT, Sivasubramani S, Ramu P, Amindala BP, Rathore A, Kishor PBK, Gupta R, Deshpande SP. Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence. Genes. 2020; 11(9):1026. https://doi.org/10.3390/genes11091026

Chicago/Turabian Style

Kiranmayee, K. N.S.U.; Hash, C. T.; Sivasubramani, S.; Ramu, P.; Amindala, Bhanu P.; Rathore, Abhishek; Kishor, P. B.K.; Gupta, Rajeev; Deshpande, Santosh P. 2020. "Fine-Mapping of Sorghum Stay-Green QTL on Chromosome10 Revealed Genes Associated with Delayed Senescence" Genes 11, no. 9: 1026. https://doi.org/10.3390/genes11091026

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