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Open AccessArticle

Transcriptome Analysis Reveals Inhibitory Effects of Lentogenic Newcastle Disease Virus on Cell Survival and Immune Function in Spleen of Commercial Layer Chicks

1
Department of Animal Science, Iowa State University, Ames, IA 50011, USA
2
Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
3
One Health Institute, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
4
Department of Animal Science, University of California, Davis, CA 95616, USA
*
Author to whom correspondence should be addressed.
Genes 2020, 11(9), 1003; https://doi.org/10.3390/genes11091003
Received: 3 August 2020 / Revised: 19 August 2020 / Accepted: 25 August 2020 / Published: 26 August 2020
(This article belongs to the Special Issue Avian Viruses and Host Responses)
As a major infectious disease in chickens, Newcastle disease virus (NDV) causes considerable economic losses in the poultry industry, especially in developing countries where there is limited access to effective vaccination. Therefore, enhancing resistance to the virus in commercial chickens through breeding is a promising way to promote poultry production. In this study, we investigated gene expression changes at 2 and 6 days post inoculation (dpi) at day 21 with a lentogenic NDV in a commercial egg-laying chicken hybrid using RNA sequencing analysis. By comparing NDV-challenged and non-challenged groups, 526 differentially expressed genes (DEGs) (false discovery rate (FDR) < 0.05) were identified at 2 dpi, and only 36 at 6 dpi. For the DEGs at 2 dpi, Ingenuity Pathway Analysis predicted inhibition of multiple signaling pathways in response to NDV that regulate immune cell development and activity, neurogenesis, and angiogenesis. Up-regulation of interferon induced protein with tetratricopeptide repeats 5 (IFIT5) in response to NDV was consistent between the current and most previous studies. Sprouty RTK signaling antagonist 1 (SPRY1), a DEG in the current study, is in a significant quantitative trait locus associated with virus load at 6 dpi in the same population. These identified pathways and DEGs provide potential targets to further study breeding strategy to enhance NDV resistance in chickens. View Full-Text
Keywords: chicken; Newcastle disease; spleen; immune response; gene expression; RNA-seq chicken; Newcastle disease; spleen; immune response; gene expression; RNA-seq
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MDPI and ACS Style

Zhang, J.; Kaiser, M.G.; Gallardo, R.A.; Kelly, T.R.; Dekkers, J.C.M.; Zhou, H.; Lamont, S.J. Transcriptome Analysis Reveals Inhibitory Effects of Lentogenic Newcastle Disease Virus on Cell Survival and Immune Function in Spleen of Commercial Layer Chicks. Genes 2020, 11, 1003. https://doi.org/10.3390/genes11091003

AMA Style

Zhang J, Kaiser MG, Gallardo RA, Kelly TR, Dekkers JCM, Zhou H, Lamont SJ. Transcriptome Analysis Reveals Inhibitory Effects of Lentogenic Newcastle Disease Virus on Cell Survival and Immune Function in Spleen of Commercial Layer Chicks. Genes. 2020; 11(9):1003. https://doi.org/10.3390/genes11091003

Chicago/Turabian Style

Zhang, Jibin; Kaiser, Michael G.; Gallardo, Rodrigo A.; Kelly, Terra R.; Dekkers, Jack C.M.; Zhou, Huaijun; Lamont, Susan J. 2020. "Transcriptome Analysis Reveals Inhibitory Effects of Lentogenic Newcastle Disease Virus on Cell Survival and Immune Function in Spleen of Commercial Layer Chicks" Genes 11, no. 9: 1003. https://doi.org/10.3390/genes11091003

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