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Article

Prognostic Significance of RAS Mutations and P53 Expression in Cutaneous Squamous Cell Carcinomas

1
Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal
2
Institute of Molecular Pathology and Immunology of the University of Porto (Ipatimup), 4200-135 Porto, Portugal
3
Medical Faculty, University of Porto, 4200-450 Porto, Portugal
4
Department of Dermatology, Centro Hospitalar Vila Nova de Gaia, 4434-502 Vila Nova de Gaia, Portugal
5
Department of Pathology, Centro Hospitalar São João, 4200-450 Porto, Portugal
6
Department of Pathology, Centro Hospitalar Vila Nova de Gaia, 4434-502 Vila Nova de Gaia, Portugal
*
Author to whom correspondence should be addressed.
Genes 2020, 11(7), 751; https://doi.org/10.3390/genes11070751
Received: 21 May 2020 / Revised: 24 June 2020 / Accepted: 30 June 2020 / Published: 6 July 2020
(This article belongs to the Special Issue Molecular Biomarkers in Solid Tumors)
TP53 is considered the most commonly-altered gene in cutaneous squamous cell carcinoma (cSCC). Conversely, RAS mutations have been reported in a low percentage of cSCC. The objective of our study was to evaluate the frequency of p53 expression and RAS mutations in cSCC and correlate them with clinicopathological features and patient outcome. We performed immunohistochemistry for p53 and genetic profiling for RAS mutations in a retrospective series of cSCC. The predictive value of p53 expression, RAS mutations, and clinicopathological parameters was assessed using logistic regression models. The overall frequency of RAS mutations was 9.3% (15/162), and 82.1% of the cases (133/162) had p53 overexpression. RAS mutations rate was 3.2% (1/31) of in situ cSCCs and 10.7% (14/131) of invasive cSCCs. RAS mutations were more frequently associated with an infiltrative than an expansive pattern of invasion (p = 0.046). p53 overexpression was a predictor of recurrence in the univariate analysis. Our results indicate that RAS mutations associate with features of local aggressiveness. Larger studies with more recurrent and metastatic cSCCs are necessary to further address the prognostic significance of p53 overexpression in patients’ risk stratification. View Full-Text
Keywords: RAS; p53; mutation; expression; prognostic biomarker; prognosis; biomarker; cutaneous squamous cell carcinoma; recurrence; metastases; outcome RAS; p53; mutation; expression; prognostic biomarker; prognosis; biomarker; cutaneous squamous cell carcinoma; recurrence; metastases; outcome
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MDPI and ACS Style

Campos, M.A.; Macedo, S.; Fernandes, M.S.; Pestana, A.; Pardal, J.; Batista, R.; Vinagre, J.; Sanches, A.; Baptista, A.; Lopes, J.M.; Soares, P. Prognostic Significance of RAS Mutations and P53 Expression in Cutaneous Squamous Cell Carcinomas. Genes 2020, 11, 751. https://doi.org/10.3390/genes11070751

AMA Style

Campos MA, Macedo S, Fernandes MS, Pestana A, Pardal J, Batista R, Vinagre J, Sanches A, Baptista A, Lopes JM, Soares P. Prognostic Significance of RAS Mutations and P53 Expression in Cutaneous Squamous Cell Carcinomas. Genes. 2020; 11(7):751. https://doi.org/10.3390/genes11070751

Chicago/Turabian Style

Campos, Manuel A., Sofia Macedo, Margarida S. Fernandes, Ana Pestana, Joana Pardal, Rui Batista, João Vinagre, Agostinho Sanches, Armando Baptista, José M. Lopes, and Paula Soares. 2020. "Prognostic Significance of RAS Mutations and P53 Expression in Cutaneous Squamous Cell Carcinomas" Genes 11, no. 7: 751. https://doi.org/10.3390/genes11070751

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