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Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells

Department of Environmental & Radiological Health Sciences, Colorado State University, 1618 Campus Delivery, Fort Collins, CO 80523, USA
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Genes 2020, 11(6), 646; https://doi.org/10.3390/genes11060646
Received: 5 May 2020 / Revised: 4 June 2020 / Accepted: 8 June 2020 / Published: 11 June 2020
(This article belongs to the Special Issue DNA and Chromosomal Lesions in Tumorigenesis)
Phototherapy using narrowband ultraviolet-B (NB-UVB) has been shown to be more effective than conventional broadband UVB (BB-UVB) in treating a variety of skin diseases. To assess the difference in carcinogenic potential between NB-UVB and BB-UVB, we investigated the cytotoxicity via colony formation assay, genotoxicity via sister chromatid exchange (SCE) assay, mutagenicity via hypoxanthine phosphoribosyltransferase (HPRT) mutation assay, as well as cyclobutane pyrimidine dimer (CPD) formation and reactive oxygen species (ROS) generation in Chinese hamster ovary (CHO) and their NER mutant cells. The radiation dose required to reduce survival to 10% (D10 value) demonstrated BB-UVB was 10 times more cytotoxic than NB-UVB, and revealed that NB-UVB also induces DNA damage repaired by nucleotide excision repair. We also found that BB-UVB more efficiently induced SCEs and HPRT mutations per absorbed energy dosage (J/m2) than NB-UVB. However, SCE and HPRT mutation frequencies were observed to rise in noncytotoxic dosages of NB-UVB exposure. BB-UVB and NB-UVB both produced a significant increase in CPD formation and ROS formation (p < 0.05); however, higher dosages were required for NB-UVB. These results suggest that NB-UVB is less cytotoxic and genotoxic than BB-UVB, but can still produce genotoxic effects even at noncytotoxic doses. View Full-Text
Keywords: narrowband UVB; broadband UVB; cytotoxicity; HPRT; SCE; DNA damage narrowband UVB; broadband UVB; cytotoxicity; HPRT; SCE; DNA damage
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Buglewicz, D.J.; Mussallem, J.T.; Haskins, A.H.; Su, C.; Maeda, J.; Kato, T.A. Cytotoxicity and Mutagenicity of Narrowband UVB to Mammalian Cells. Genes 2020, 11, 646.

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