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Transcriptome Profiling of Haloxylon persicum (Bunge ex Boiss and Buhse) an Endangered Plant Species under PEG-Induced Drought Stress

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Department of Integrative Agriculture, College of Food and Agriculture, United Arab Emirates University, P.O. Box. Al-Ain 15551, UAE
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Khalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, P.O. Box. Al Ain 15551, UAE
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Institute of Computer Science, University of Tartu, 50409 Tartu, Estonia
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Celixa, Bangalore, Karnataka 560020, India
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School of Biological Sciences, Universiti Sains Malaysia (USM), Minden Heights, Georgetown, Penang 11800, Malaysia
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Department of Biology, College of Science, United Arab Emirates University, P.O. Box. Al Ain 15551, UAE
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Authors to whom correspondence should be addressed.
Co-first author.
Genes 2020, 11(6), 640; https://doi.org/10.3390/genes11060640
Received: 2 May 2020 / Revised: 3 June 2020 / Accepted: 5 June 2020 / Published: 10 June 2020
(This article belongs to the Section Plant Genetics and Genomics)
Haloxylon persicum is an endangered western Asiatic desert plant species, which survives under extreme environmental conditions. In this study, we focused on transcriptome analysis of H. persicum to understand the molecular mechanisms associated with drought tolerance. Two different periods of polyethylene glycol (PEG)-induced drought stress (48 h and 72 h) were imposed on H. persicum under in vitro conditions, which resulted in 18 million reads, subsequently assembled by de novo method with more than 8000 transcripts in each treatment. The N50 values were 1437, 1467, and 1524 for the control sample, 48 h samples, and 72 h samples, respectively. The gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis resulted in enrichment of mitogen-activated protein kinase (MAPK) and plant hormone signal transduction pathways under PEG-induced drought conditions. The differential gene expression analysis (DGEs) revealed significant changes in the expression pattern between the control and the treated samples. The KEGG analysis resulted in mapping transcripts with 138 different pathways reported in plants. The differential expression of drought-responsive transcription factors depicts the possible signaling cascades involved in drought tolerance. The present study provides greater insight into the fundamental transcriptome reprogramming of desert plants under drought. View Full-Text
Keywords: Haloxylon persicum; white saxaul; drought resistant; de novo transcriptome Haloxylon persicum; white saxaul; drought resistant; de novo transcriptome
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Thayale Purayil, F.; Rajashekar, B.; S. Kurup, S.; Cheruth, A.J.; Subramaniam, S.; Hassan Tawfik, N.; M.A. Amiri, K. Transcriptome Profiling of Haloxylon persicum (Bunge ex Boiss and Buhse) an Endangered Plant Species under PEG-Induced Drought Stress. Genes 2020, 11, 640.

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