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De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (Trichosurus vulpecula) in New Zealand: Transcriptome Diversity after Decades of Population Control

1
Center for Ecological Genomics and Wildlife Conservation, University of Johannesburg, Auckland Park 2006, South Africa
2
Department of Pest-management and Conservation, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand
*
Author to whom correspondence should be addressed.
Genes 2020, 11(4), 436; https://doi.org/10.3390/genes11040436
Received: 27 February 2020 / Revised: 9 April 2020 / Accepted: 10 April 2020 / Published: 17 April 2020
(This article belongs to the Section Animal Genetics and Genomics)
The common brushtail possum (Trichosurus vulpecula), introduced from Australia in the mid-nineteenth century, is an invasive species in New Zealand where it is widespread and forms the largest self-sustained reservoir of bovine tuberculosis (Mycobacterium bovis) among wild populations. Conservation and agricultural authorities regularly apply a series of population control measures to suppress brushtail possum populations. The evolutionary consequence of more than half a century of intensive population control operations on the species’ genomic diversity and population structure is hindered by a paucity of available genomic resources. This study is the first to characterise the functional content and diversity of brushtail possum liver and brain cerebral cortex transcriptomes. Raw sequences from hepatic cells and cerebral cortex were assembled into 58,001 and 64,735 transcripts respectively. Functional annotation and polymorphism assignment of the assembled transcripts demonstrated a considerable level of variation in the core metabolic pathways that represent potential targets for selection pressure exerted by chemical toxicants. This study suggests that the brushtail possum population in New Zealand harbours considerable variation in metabolic pathways that could potentially promote the development of tolerance against chemical toxicants. View Full-Text
Keywords: De novo transcriptome assembly; common brushtail possum; liver; brain cerebral cortex; drug and xenobiotic metabolism; chemical toxicants De novo transcriptome assembly; common brushtail possum; liver; brain cerebral cortex; drug and xenobiotic metabolism; chemical toxicants
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MDPI and ACS Style

Emami-Khoyi, A.; Parbhu, S.P.; Ross, J.G.; Murphy, E.C.; Bothwell, J.; Monsanto, D.M.; Vuuren, B.J.v.; Teske, P.R.; Paterson, A.M. De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (Trichosurus vulpecula) in New Zealand: Transcriptome Diversity after Decades of Population Control. Genes 2020, 11, 436. https://doi.org/10.3390/genes11040436

AMA Style

Emami-Khoyi A, Parbhu SP, Ross JG, Murphy EC, Bothwell J, Monsanto DM, Vuuren BJv, Teske PR, Paterson AM. De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (Trichosurus vulpecula) in New Zealand: Transcriptome Diversity after Decades of Population Control. Genes. 2020; 11(4):436. https://doi.org/10.3390/genes11040436

Chicago/Turabian Style

Emami-Khoyi, Arsalan, Shilpa Pradeep Parbhu, James G. Ross, Elaine C. Murphy, Jennifer Bothwell, Daniela M. Monsanto, Bettine Jansen van Vuuren, Peter R. Teske, and Adrian M. Paterson. 2020. "De Novo Transcriptome Assembly and Annotation of Liver and Brain Tissues of Common Brushtail Possums (Trichosurus vulpecula) in New Zealand: Transcriptome Diversity after Decades of Population Control" Genes 11, no. 4: 436. https://doi.org/10.3390/genes11040436

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