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Volume 11
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10.3390/genes11030336
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Article

Prolactin-Responsive Circular RNA circHIPK3 Promotes Proliferation of Mammary Epithelial Cells from Dairy Cow

by
Jia Liu
1,†,
MoLan Zhang
1,†,
DeWei Li
1,
MengLu Li
1,
LingHao Kong
1,
MengWen Cao
1,
YanHong Wang
1,
ChengChuang Song
1,
XingTang Fang
1,*,
Hong Chen
1,
Hu Xu
2 and
ChunLei Zhang
1,*
1
Institute of Cellular and Molecular Biology, School of Life Science, Jiangsu Normal University, Xuzhou 221116, Jiangsu, China
2
Kunming Police Dog Base of the Ministry of Public Security, Kunming 650204, Yunnan, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally.
Genes 2020, 11(3), 336; https://doi.org/10.3390/genes11030336
Submission received: 8 February 2020 / Revised: 18 March 2020 / Accepted: 18 March 2020 / Published: 20 March 2020
(This article belongs to the Section Animal Genetics and Genomics)
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Abstract

The highly expressed circHIPK3 is a circular RNA that has been previously reported to regulate the growth of human cells. In this study, we found an increased expression of circHIPK3 in bovine mammary epithelial cells treated with prolactin (PRL) in high-throughput sequencing data. Thus, we further investigated the effect of circHIPK3 on the proliferation and differentiation of mammary epithelial cells. We used qRT-PCR/Cell Counting Kit-8 (CCK-8) and a Western blotting analysis to evaluate the effects on cell proliferation. We found that circHIPK3 promotes the proliferation of mammary epithelial cells. The STAT5 signaling pathway was previously associated with the prolactin response and when the STAT5 was suppressed, the expression of circHIPK3 decreased. The results suggest that the response to prolactin and the associated STAT5 signaling pathway affect the expression of circHIPK3, which subsequently affects the proliferation of mammary epithelial cells in dairy cows.
Keywords: PRL; circHIPK3; STAT5; splicing factors; lactation regulation PRL; circHIPK3; STAT5; splicing factors; lactation regulation

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MDPI and ACS Style

Liu, J.; Zhang, M.; Li, D.; Li, M.; Kong, L.; Cao, M.; Wang, Y.; Song, C.; Fang, X.; Chen, H.; et al. Prolactin-Responsive Circular RNA circHIPK3 Promotes Proliferation of Mammary Epithelial Cells from Dairy Cow. Genes 2020, 11, 336. https://doi.org/10.3390/genes11030336

AMA Style

Liu J, Zhang M, Li D, Li M, Kong L, Cao M, Wang Y, Song C, Fang X, Chen H, et al. Prolactin-Responsive Circular RNA circHIPK3 Promotes Proliferation of Mammary Epithelial Cells from Dairy Cow. Genes. 2020; 11(3):336. https://doi.org/10.3390/genes11030336

Chicago/Turabian Style

Liu, Jia, MoLan Zhang, DeWei Li, MengLu Li, LingHao Kong, MengWen Cao, YanHong Wang, ChengChuang Song, XingTang Fang, Hong Chen, and et al. 2020. "Prolactin-Responsive Circular RNA circHIPK3 Promotes Proliferation of Mammary Epithelial Cells from Dairy Cow" Genes 11, no. 3: 336. https://doi.org/10.3390/genes11030336

APA Style

Liu, J., Zhang, M., Li, D., Li, M., Kong, L., Cao, M., Wang, Y., Song, C., Fang, X., Chen, H., Xu, H., & Zhang, C. (2020). Prolactin-Responsive Circular RNA circHIPK3 Promotes Proliferation of Mammary Epithelial Cells from Dairy Cow. Genes, 11(3), 336. https://doi.org/10.3390/genes11030336

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