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Volume 11
Issue 3
10.3390/genes11030321
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Article

Comparative Transcriptomics Reveals Clues for Differences in Pathogenicity between Hysterothylacium aduncum, Anisakis simplex sensu stricto and Anisakis pegreffii

by
Serena Cavallero
1,
Fabrizio Lombardo
1,
Marco Salvemini
2,
Antonella Pizzarelli
1,
Cinzia Cantacessi
3 and
Stefano D’Amelio
1,*
1
Department of Public Health and Infectious Diseases, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
2
Department of Biology, University of Naples Federico II, Corso Umberto I, 40, 80138 Naples, Italy
3
Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge CB3 0ES, UK
*
Author to whom correspondence should be addressed.
Genes 2020, 11(3), 321; https://doi.org/10.3390/genes11030321
Submission received: 3 March 2020 / Accepted: 16 March 2020 / Published: 18 March 2020
(This article belongs to the Special Issue Novel Omics Studies on Anisakid Nematodes)
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Abstract

Ascaridoid nematodes are widespread in marine fishes. Despite their major socioeconomic importance, mechanisms associated to the fish-borne zoonotic disease anisakiasis are still obscure. RNA-Seq and de-novo assembly were herein applied to RNA extracted from larvae and dissected pharynx of Hysterothylacium aduncum (HA), a non-pathogenic nematode. Assembled transcripts in HA were annotated and compared to the transcriptomes of the zoonotic species Anisakis simplex sensu stricto (AS) and Anisakis pegreffii (AP). Approximately 60,000,000 single-end reads were generated for HA, AS and AP. Transcripts in HA encoded for 30,254 putative peptides while AS and AP encoded for 20,574 and 20,840 putative peptides, respectively. Differential gene expression analyses yielded 471, 612 and 526 transcripts up regulated in the pharynx of HA, AS and AP. The transcriptomes of larvae and pharynx of HA were enriched in transcripts encoding collagen, peptidases, ribosomal proteins and in heat-shock motifs. Transcripts encoding proteolytic enzymes, anesthetics, inhibitors of primary hemostasis and virulence factors, anticoagulants and immunomodulatory peptides were up-regulated in AS and AP pharynx. This study represents the first transcriptomic characterization of a marine parasitic nematode commonly recovered in fish and probably of negligible concern for public health.
Keywords: Hysterothylacium; Anisakis; transcriptome; pharyngeal transcripts; DEGs Hysterothylacium; Anisakis; transcriptome; pharyngeal transcripts; DEGs

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MDPI and ACS Style

Cavallero, S.; Lombardo, F.; Salvemini, M.; Pizzarelli, A.; Cantacessi, C.; D’Amelio, S. Comparative Transcriptomics Reveals Clues for Differences in Pathogenicity between Hysterothylacium aduncum, Anisakis simplex sensu stricto and Anisakis pegreffii. Genes 2020, 11, 321. https://doi.org/10.3390/genes11030321

AMA Style

Cavallero S, Lombardo F, Salvemini M, Pizzarelli A, Cantacessi C, D’Amelio S. Comparative Transcriptomics Reveals Clues for Differences in Pathogenicity between Hysterothylacium aduncum, Anisakis simplex sensu stricto and Anisakis pegreffii. Genes. 2020; 11(3):321. https://doi.org/10.3390/genes11030321

Chicago/Turabian Style

Cavallero, Serena, Fabrizio Lombardo, Marco Salvemini, Antonella Pizzarelli, Cinzia Cantacessi, and Stefano D’Amelio. 2020. "Comparative Transcriptomics Reveals Clues for Differences in Pathogenicity between Hysterothylacium aduncum, Anisakis simplex sensu stricto and Anisakis pegreffii" Genes 11, no. 3: 321. https://doi.org/10.3390/genes11030321

APA Style

Cavallero, S., Lombardo, F., Salvemini, M., Pizzarelli, A., Cantacessi, C., & D’Amelio, S. (2020). Comparative Transcriptomics Reveals Clues for Differences in Pathogenicity between Hysterothylacium aduncum, Anisakis simplex sensu stricto and Anisakis pegreffii. Genes, 11(3), 321. https://doi.org/10.3390/genes11030321

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