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Open AccessArticle

Proteomics Reveals Distinct Changes Associated with Increased Gamma Radiation Resistance in the Black Yeast Exophiala dermatitidis

1
Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375, USA
2
National Research Council, Postdoctoral Fellowship Program, US Naval Research Laboratory, Washington, DC 20744, USA
3
Virginia Tech Carilion School of Medicine, Roanoke, VA 24016, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Genes 2020, 11(10), 1128; https://doi.org/10.3390/genes11101128
Received: 27 August 2020 / Revised: 16 September 2020 / Accepted: 18 September 2020 / Published: 25 September 2020
The yeast Exophiala dermatitidis exhibits high resistance to γ-radiation in comparison to many other fungi. Several aspects of this phenotype have been characterized, including its dependence on homologous recombination for the repair of radiation-induced DNA damage, and the transcriptomic response invoked by acute γ-radiation exposure in this organism. However, these findings have yet to identify unique γ-radiation exposure survival strategies—many genes that are induced by γ-radiation exposure do not appear to be important for recovery, and the homologous recombination machinery of this organism is not unique compared to more sensitive species. To identify features associated with γ-radiation resistance, here we characterized the proteomes of two E. dermatitidis strains—the wild type and a hyper-resistant strain developed through adaptive laboratory evolution—before and after γ-radiation exposure. The results demonstrate that protein intensities do not change substantially in response to this stress. Rather, the increased resistance exhibited by the evolved strain may be due in part to increased basal levels of single-stranded binding proteins and a large increase in ribosomal content, possibly allowing for a more robust, induced response during recovery. This experiment provides evidence enabling us to focus on DNA replication, protein production, and ribosome levels for further studies into the mechanism of γ-radiation resistance in E. dermatitidis and other fungi. View Full-Text
Keywords: black yeast; melanin; proteomics; radiobiology; translation black yeast; melanin; proteomics; radiobiology; translation
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MDPI and ACS Style

Schultzhaus, Z.S.; Schultzhaus, J.N.; Romsdahl, J.; Chen, A.; Hervey IV, W.J.; Leary, D.H.; Wang, Z. Proteomics Reveals Distinct Changes Associated with Increased Gamma Radiation Resistance in the Black Yeast Exophiala dermatitidis. Genes 2020, 11, 1128. https://doi.org/10.3390/genes11101128

AMA Style

Schultzhaus ZS, Schultzhaus JN, Romsdahl J, Chen A, Hervey IV WJ, Leary DH, Wang Z. Proteomics Reveals Distinct Changes Associated with Increased Gamma Radiation Resistance in the Black Yeast Exophiala dermatitidis. Genes. 2020; 11(10):1128. https://doi.org/10.3390/genes11101128

Chicago/Turabian Style

Schultzhaus, Zachary S.; Schultzhaus, Janna N.; Romsdahl, Jillian; Chen, Amy; Hervey IV, W. J.; Leary, Dagmar H.; Wang, Zheng. 2020. "Proteomics Reveals Distinct Changes Associated with Increased Gamma Radiation Resistance in the Black Yeast Exophiala dermatitidis" Genes 11, no. 10: 1128. https://doi.org/10.3390/genes11101128

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