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Open AccessArticle

Expression Analysis of XTH in Stem Swelling of Stem Mustard and Selection of Reference Genes

1
College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China
2
Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, China
*
Author to whom correspondence should be addressed.
Genes 2020, 11(1), 113; https://doi.org/10.3390/genes11010113
Received: 28 November 2019 / Revised: 8 January 2020 / Accepted: 15 January 2020 / Published: 20 January 2020
(This article belongs to the Section Plant Genetics and Genomics)
Accurate analysis of gene expression requires selection of appropriate reference genes. In this study, we report analysis of eight candidate reference genes (ACTIN, UBQ, EF-1α, UBC, IF-4α, TUB, PP2A, and HIS), which were screened from the genome and transcriptome data in Brassica juncea. Four statistical analysis softwares geNorm, NormFinder, BestKeeper, and RefFinder were used to test the reliability and stability of gene expression of the reference genes. To further validate the stability of reference genes, the expression levels of two CYCD3 genes (BjuB045330 and BjuA003219) were studied. In addition, all genes in the xyloglucan endotransglucosylase/hydrolase (XTH) family were identified in B. juncea and their patterns at different periods of stem enlargement were analyzed. Results indicated that UBC and TUB genes showed stable levels of expression and are recommended for future research. In addition, XTH genes were involved in regulation of stem enlargement expression. These results provide new insights for future research aiming at exploring important functional genes, their expression patterns and regulatory mechanisms for mustard development. View Full-Text
Keywords: stem mustard; reference gene; qPCR; Brassica juncea; XTH genes stem mustard; reference gene; qPCR; Brassica juncea; XTH genes
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Li, M.; Xie, F.; He, Q.; Li, J.; Liu, J.; Sun, B.; Luo, Y.; Zhang, Y.; Chen, Q.; Zhang, F.; Gong, R.; Wang, Y.; Wang, X.; Tang, H. Expression Analysis of XTH in Stem Swelling of Stem Mustard and Selection of Reference Genes. Genes 2020, 11, 113.

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