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Article

Identification of TCP13 as an Upstream Regulator of ATHB12 during Leaf Development

1
Department of Biological Science and Research Institute of Women’s Health, Sookmyung Women’s University, Seoul 04310, Korea
2
College of Pharmacy, Sookmyung Women’s University, Seoul 04310, Korea
3
Bioproduction Department of Molecular Biotechnology, Dong-A University, Busan 49315, Korea
4
Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan
5
Institute for Environmental Science and Technology (IEST), Saitama University, Saitama 338-8570, Japan
*
Author to whom correspondence should be addressed.
Genes 2019, 10(9), 644; https://doi.org/10.3390/genes10090644
Received: 2 August 2019 / Revised: 20 August 2019 / Accepted: 21 August 2019 / Published: 26 August 2019
(This article belongs to the Section Plant Genetics and Genomics)
Leaves grow by distinct phases controlled by gene regulatory networks including many transcription factors. Arabidopsis thaliana homeobox 12 (ATHB12) promotes leaf growth especially during the cell expansion phase. In this study, we identify TCP13, a member of the TCP transcription factor family, as an upstream inhibitor of ATHB12. Yeast one-hybrid screening using a 1.2-kb upstream region of ATHB12 resulted in the isolation of TCP13 as well as other transcription factors. Transgenic plants constitutively expressing TCP13 displays a significant reduction in leaf cell size especially during the cell expansion period, while repression of TCP13 and its paralogs (TCP5 and TCP17) result in enlarged leaf cells, indicating that TCP13 and its paralogs inhibit leaf development, mainly at the cell expansion phase. Its expression pattern during leaf expansion phase is opposite to ATHB12 expression. Consistently, the expression of ATHB12 and its downstream genes decreases when TCP13 was overexpressed, and increases when the expression of TCP13 and its paralogs is repressed. In chromatin immunoprecipitation assays using TCP13-GFP plants, a fragment of the ATHB12 upstream region that contains the consensus sequence for TCP binding is strongly enriched. Taken together, these findings indicate that TCP13 and its paralogs inhibit leaf growth by repressing ATHB12 expression. View Full-Text
Keywords: leaf development; ATHB12; TCP13; cell expansion; upstream regulator leaf development; ATHB12; TCP13; cell expansion; upstream regulator
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MDPI and ACS Style

Hur, Y.-S.; Kim, J.; Kim, S.; Son, O.; Kim, W.-Y.; Kim, G.-T.; Ohme-Takagi, M.; Cheon, C.-I. Identification of TCP13 as an Upstream Regulator of ATHB12 during Leaf Development. Genes 2019, 10, 644. https://doi.org/10.3390/genes10090644

AMA Style

Hur Y-S, Kim J, Kim S, Son O, Kim W-Y, Kim G-T, Ohme-Takagi M, Cheon C-I. Identification of TCP13 as an Upstream Regulator of ATHB12 during Leaf Development. Genes. 2019; 10(9):644. https://doi.org/10.3390/genes10090644

Chicago/Turabian Style

Hur, Yoon-Sun, Jiyoung Kim, Sunghan Kim, Ora Son, Woo-Young Kim, Gyung-Tae Kim, Masaru Ohme-Takagi, and Choong-Ill Cheon. 2019. "Identification of TCP13 as an Upstream Regulator of ATHB12 during Leaf Development" Genes 10, no. 9: 644. https://doi.org/10.3390/genes10090644

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