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Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4

1, 2 and 1,*
1
College of Animal Sciences, Jilin University, Changchun 130062, China
2
Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(8), 615; https://doi.org/10.3390/genes10080615
Received: 16 May 2019 / Revised: 9 August 2019 / Accepted: 9 August 2019 / Published: 13 August 2019
(This article belongs to the Special Issue Domestication Genetics)
PDF [1260 KB, uploaded 13 August 2019]

Abstract

Retinol binding protein 4 (RBP4), mainly secreted by the liver and adipocytes, is a transporter of vitamin A. RBP4 has been shown to be involved in several pathophysiological processes, such as obesity, insulin resistance, and cardiovascular risk. Reports have indicated the high expression levels of RBP4 in cystic follicles. However, the role of RBP4 in mammalian follicular granulosa cells (GCs) remains largely unknown. To illustrate the molecular pathways associated with the effects of RBP4 on GCs, we used high-throughput sequencing to detect differential gene expression in GCs overexpressing RBP4. A total of 113 differentially expressed genes (DEGs) were identified in RBP4-overexpressing GCs, and they included 71 upregulated and 42 downregulated genes. The differential expressions of the top 10 DEGs were further confirmed by real-time quantitative polymerase chain reaction. Pathway analysis indicated that the DEGs are mostly involved in oxidative phosphorylation, Parkinson’s disease, non-alcoholic fatty liver disease, Huntington’s disease, cardiac muscle contraction, Alzheimer’s disease, fatty acid biosynthesis, AMP-activated protein kinase signaling pathway, and insulin signaling pathway. Genes in these pathways should be useful for future studies on GCs. Altogether, the results of our study establish a framework for understanding the potential functions of RBP4 in porcine GCs.
Keywords: granulosa cells (GCs); high-throughput sequencing; pLVX-IRES-ZsGreen vector; retinol binding protein 4 (RBP4) granulosa cells (GCs); high-throughput sequencing; pLVX-IRES-ZsGreen vector; retinol binding protein 4 (RBP4)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Zhao, Y.; Li, C.; Zhou, X. Transcriptomic Analysis of Porcine Granulosa Cells Overexpressing Retinol Binding Protein 4. Genes 2019, 10, 615.

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