Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant (Solanum melongena L.)
AbstractWhole-genome sequences of four EMS (ethyl methanesulfonate)-induced eggplant mutants were analyzed to identify genome-wide mutations. In total, 173.01 GB of paired-end reads were obtained for four EMS-induced mutants and (WT) wild type and 1,076,010 SNPs (single nucleotide polymorphisms) and 183,421 indels were identified. The most common mutation type was C/G to T/A transitions followed by A/T to G/C transitions. The mean densities were one SNP per 1.3 to 2.6 Mb. The effect of mutations on gene function was annotated and only 7.2% were determined to be deleterious. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed 10 and 11 genes, which were nonsynonymous mutation or frameshift deletion in 48-5 and L6-5 involved in the anthocyanin biosynthesis or flavone and flavonol biosynthesis. QRT-PCR results showed that only the Sme2.5_06210.1_g00004.1, which was annotated as UFGT (Flavonoid galactosidase transferase), expression significantly decreased in the L6-5 mutant compared with the WT. Also, the Sme2.5_06210.1_g00004.1 expression was lower in the colorless eggplant compared with colorful eggplant in the natural eggplant cultivar. These results suggest that Sme2.5_06210.1_g00004.1 may play a key role in eggplant anthocyanin synthesis. View Full-Text
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Description: Table S1: The primers used for RT-PCT.; Table S2: Pathway assignment based on high-impact mutation gene in the four EMS-induced mutants.; Table S3: The gene ID involved in the anthocyanin synthesis. Figure S1: The nucleotide and protein sequence alignment of Sme2.5_06210.1_g00004.1 and Sme2.5_00228.1_g00013.1. (A), The nucleotide sequence alignment. (B), protein sequence alignment.; Figure S2: The Sme2.5_06210.1_g00004.1 expression in four natural eggplant population. WS, the eggplant was white skin, PS, the eggplant was purple skin, PBS, the eggplant was purple black skFin, GS, the eggplant was green skin.
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Xiao, X.-O.; Lin, W.; Li, K.; Feng, X.; Jin, H.; Zou, H. Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant (Solanum melongena L.). Genes 2019, 10, 595.
Xiao X-O, Lin W, Li K, Feng X, Jin H, Zou H. Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant (Solanum melongena L.). Genes. 2019; 10(8):595.Chicago/Turabian Style
Xiao, Xi-ou; Lin, Wenqiu; Li, Ke; Feng, Xuefeng; Jin, Hui; Zou, Huafeng. 2019. "Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant (Solanum melongena L.)." Genes 10, no. 8: 595.
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