Next Article in Journal
Multiplex CRISPR Mutagenesis of the Serine/Arginine-Rich (SR) Gene Family in Rice
Previous Article in Journal
Developmental Regulation of the Expression of Amaryllidaceae Alkaloid Biosynthetic Genes in Narcissus papyraceus
Open AccessArticle

Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant (Solanum melongena L.)

by 1,2,*,†, 1,2, 1,2, 1,2, 1,2 and 1,2
1
South Subtropical Crop Research Institute Chinese Academy of Tropical Agricultural Sciences, Zhanjiang City 524091, China
2
Zhanjiang City Key Laboratory for Tropical Crops Genetic Improvement, Guangdong Province, Zhanjiang City 524091, China
*
Author to whom correspondence should be addressed.
Present Address: Room 507, Huxiu Road No.1, Mazhang District, Zhanjiang 52491, Guangdong Province, China.
Genes 2019, 10(8), 595; https://doi.org/10.3390/genes10080595
Received: 14 June 2019 / Revised: 19 July 2019 / Accepted: 1 August 2019 / Published: 7 August 2019
(This article belongs to the Section Plant Genetics and Genomics)
Whole-genome sequences of four EMS (ethyl methanesulfonate)-induced eggplant mutants were analyzed to identify genome-wide mutations. In total, 173.01 GB of paired-end reads were obtained for four EMS-induced mutants and (WT) wild type and 1,076,010 SNPs (single nucleotide polymorphisms) and 183,421 indels were identified. The most common mutation type was C/G to T/A transitions followed by A/T to G/C transitions. The mean densities were one SNP per 1.3 to 2.6 Mb. The effect of mutations on gene function was annotated and only 7.2% were determined to be deleterious. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed 10 and 11 genes, which were nonsynonymous mutation or frameshift deletion in 48-5 and L6-5 involved in the anthocyanin biosynthesis or flavone and flavonol biosynthesis. QRT-PCR results showed that only the Sme2.5_06210.1_g00004.1, which was annotated as UFGT (Flavonoid galactosidase transferase), expression significantly decreased in the L6-5 mutant compared with the WT. Also, the Sme2.5_06210.1_g00004.1 expression was lower in the colorless eggplant compared with colorful eggplant in the natural eggplant cultivar. These results suggest that Sme2.5_06210.1_g00004.1 may play a key role in eggplant anthocyanin synthesis. View Full-Text
Keywords: eggplant; ethyl methanesulfonate mutation; fruit color; gene function; whole-genome re-sequencing eggplant; ethyl methanesulfonate mutation; fruit color; gene function; whole-genome re-sequencing
Show Figures

Figure 1

MDPI and ACS Style

Xiao, X.-O.; Lin, W.; Li, K.; Feng, X.; Jin, H.; Zou, H. Genome-Wide Analysis of Artificial Mutations Induced by Ethyl Methanesulfonate in the Eggplant (Solanum melongena L.). Genes 2019, 10, 595.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
  • Supplementary File 1:

    RAR-Document (RAR, 5665 KB)

  • Externally hosted supplementary file 1
    Doi: https://susy.mdpi.com/user/manuscripts/resubmit/b76437f105f45b07042b3ee2bca1683d#resubmit-manuscript-section
    Link: https://susy.mdpi.com/user/manuscripts/resubmit/b76437f105f45b07042b3ee2bca1683d#resubmit-manuscript-section
    Description: Table S1: The primers used for RT-PCT.; Table S2: Pathway assignment based on high-impact mutation gene in the four EMS-induced mutants.; Table S3: The gene ID involved in the anthocyanin synthesis. Figure S1: The nucleotide and protein sequence alignment of Sme2.5_06210.1_g00004.1 and Sme2.5_00228.1_g00013.1. (A), The nucleotide sequence alignment. (B), protein sequence alignment.; Figure S2: The Sme2.5_06210.1_g00004.1 expression in four natural eggplant population. WS, the eggplant was white skin, PS, the eggplant was purple skin, PBS, the eggplant was purple black skFin, GS, the eggplant was green skin.
Back to TopTop