Next Article in Journal
Thyroid Cancer: The Quest for Genetic Susceptibility Involving DNA Repair Genes
Previous Article in Journal
Untangling the Genetics of Human Longevity—A Challenging Quest
Open AccessArticle

Identification of a Novel Melon Transcription Factor CmNAC60 as a Potential Regulator of Leaf Senescence

1
Key Laboratory for Tobacco Gene Resources, Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao 266101, China
2
College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
*
Author to whom correspondence should be addressed.
Genes 2019, 10(8), 584; https://doi.org/10.3390/genes10080584
Received: 7 June 2019 / Revised: 25 July 2019 / Accepted: 30 July 2019 / Published: 31 July 2019
(This article belongs to the Section Plant Genetics and Genomics)
  |  
PDF [5027 KB, uploaded 13 August 2019]
  |  

Abstract

NAC transcription factors (TFs) play important roles in plants’ responses to abiotic stresses and developmental processes, including leaf senescence. Oriental melon (Cucumis melo var. makuwa Makino) is an important vegetable crop in China and eastern Asia countries. However, little is known about the functions of the melon NAC family members. In this study, a phylogenetic tree was constructed to show that CmNAC60 and the senescence regulator AtNAP were in the same cluster, which implied that CmNAC60 might be a NAC related to leaf senescence. The expression analysis of CmNAC60 in different melon organs showed that the expression of CmNAC60 was highest in the male flowers and lowest in the hypocotyl. In addition, the expression level of CmNAC60 in the senescing leaves was significantly higher than in the non-senescing leaves. Similarly, the expression level of CmNAC60 in the dark-treated leaves was significantly higher than in the untreated leaves. Furthermore, the subcellular localization and transcriptional activation assays indicated that CmNAC60 was a nucleus localized NAC transcription factor with a C-terminal transactivation domain. An analysis of the tissue specific expression showed that the promoter of CmNAC60 may contain cis-acting regulatory elements responsive to leaf senescence. CmNAC60 overexpressing lines of Arabidopsis showed a precocious senescence compared with the wild type (WT). Collectively, our results showed that CmNAC60 was associated with leaf senescence, and could be potentially utilized in molecular breeding to improve melon yield or to extend the postharvest shelf life by delaying leaf senescence. View Full-Text
Keywords: NAC transcription factor; leaf senescence; Oriental melon; CmNAC60 NAC transcription factor; leaf senescence; Oriental melon; CmNAC60
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Cao, S.; Zhang, Z.; Wang, C.; Li, X.; Guo, C.; Yang, L.; Guo, Y. Identification of a Novel Melon Transcription Factor CmNAC60 as a Potential Regulator of Leaf Senescence. Genes 2019, 10, 584.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top