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Volume 10
Issue 6
10.3390/genes10060477
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Article

Genome-Wide Analysis of Long Non-Coding RNA Profiles in Canine Oral Melanomas

by
Christophe Hitte
1,*,
Céline Le Béguec
1,
Edouard Cadieu
1,
Valentin Wucher
2,
Aline Primot
1,
Anaïs Prouteau
1,
Nadine Botherel
1,
Benoît Hédan
1,
Kerstin Lindblad-Toh
3,4,
Catherine André
1 and
Thomas Derrien
1,*
1
University of Rennes, CNRS, IGDR—UMR 6290, F-35000 Rennes, France
2
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, 08003 Barcelona, Spain
3
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
4
Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Box 582, SE-751 24 Uppsala, Sweden
*
Authors to whom correspondence should be addressed.
Genes 2019, 10(6), 477; https://doi.org/10.3390/genes10060477
Received: 29 April 2019 / Revised: 17 June 2019 / Accepted: 19 June 2019 / Published: 23 June 2019
(This article belongs to the Special Issue Canine Genetics)
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Abstract

Mucosal melanomas (MM) are rare aggressive cancers in humans, and one of the most common forms of oral cancers in dogs. Similar biological and histological features are shared between MM in both species, making dogs a powerful model for comparative oncology studies of melanomas. Although exome sequencing recently identified recurrent coding mutations in canine MM, little is known about changes in non-coding gene expression, and more particularly, in canine long non-coding RNAs (lncRNAs), which are commonly dysregulated in human cancers. Here, we sampled a large cohort (n = 52) of canine normal/tumor oral MM from three predisposed breeds (poodles, Labrador retrievers, and golden retrievers), and used deep transcriptome sequencing to identify more than 400 differentially expressed (DE) lncRNAs. We further prioritized candidate lncRNAs by comparative genomic analysis to pinpoint 26 dog–human conserved DE lncRNAs, including SOX21-AS, ZEB2-AS, and CASC15 lncRNAs. Using unsupervised co-expression network analysis with coding genes, we inferred the potential functions of the DE lncRNAs, suggesting associations with cancer-related genes, cell cycle, and carbohydrate metabolism Gene Ontology (GO) terms. Finally, we exploited our multi-breed design to identify DE lncRNAs within breeds. This study provides a unique transcriptomic resource for studying oral melanoma in dogs, and highlights lncRNAs that may potentially be diagnostic or therapeutic targets for human and veterinary medicine. View Full-Text
Keywords: mucosal melanoma; dogs; transcriptome sequencing; long non-coding RNAs (lncRNAs) mucosal melanoma; dogs; transcriptome sequencing; long non-coding RNAs (lncRNAs)
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MDPI and ACS Style

Hitte, C.; Le Béguec, C.; Cadieu, E.; Wucher, V.; Primot, A.; Prouteau, A.; Botherel, N.; Hédan, B.; Lindblad-Toh, K.; André, C.; Derrien, T. Genome-Wide Analysis of Long Non-Coding RNA Profiles in Canine Oral Melanomas. Genes 2019, 10, 477. https://doi.org/10.3390/genes10060477

AMA Style

Hitte C, Le Béguec C, Cadieu E, Wucher V, Primot A, Prouteau A, Botherel N, Hédan B, Lindblad-Toh K, André C, Derrien T. Genome-Wide Analysis of Long Non-Coding RNA Profiles in Canine Oral Melanomas. Genes. 2019; 10(6):477. https://doi.org/10.3390/genes10060477

Chicago/Turabian Style

Hitte, Christophe, Céline Le Béguec, Edouard Cadieu, Valentin Wucher, Aline Primot, Anaïs Prouteau, Nadine Botherel, Benoît Hédan, Kerstin Lindblad-Toh, Catherine André, and Thomas Derrien. 2019. "Genome-Wide Analysis of Long Non-Coding RNA Profiles in Canine Oral Melanomas" Genes 10, no. 6: 477. https://doi.org/10.3390/genes10060477

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